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| Effects of in ovo Injection of Valine on the Growth and Development of Duck (Anas platyrhynchos) Embryo and Hepatic Fat Deposition |
| ZHANG Yu, PANG Yan-Qin, ZHANG Rong, WANG Li, WANG Jia-Xiang, LI Peng, YANG Cai-Xia, DU Zhi-Qiang* |
| College of Animal Science, Yangtze University, Jingzhou 434025, China |
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Abstract Branched chain amino acids (BCAAs) including leucine, isoleucine and valine, affect the production efficiency and product quality of poultry industry. In order to further explore the molecular mechanism of BCAAs imbalance hindering the growth and development of poultry, this study established a duck (Anas platyrhynchos) embryo egg feeding model by embryo egg injection technology, analyzed its phenotype and incubation time. Furthermore, ELISA, paraffin section and qPCR techniques were used to examine the effect of valine on liver fat deposition in duck embryos. The results showed that valine treatment significantly shortened the incubation time, significantly changed the breast weight, male pancreas weight and female gonad (ovary) length of ducklings, as well as the level of insulin like growth factor 1 (IGF-1) in the serum of embryos at different stages of development (P<0.05). The number of lipid droplets in the treatment group increased significantly. The expression level of IGF-1 pathway and adipogenesis related genes also changed significantly (P<0.05) and had sex specificity. To sum up, duck embryo valine treatment can affect its growth and development, promote liver fat synthesis. This study provides a new research perspective and material for the impact of BCAAs imbalance on poultry production.
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Received: 29 December 2022
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Corresponding Authors:
*zhqdu@yangtzeu.edu.cn
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