|
|
|
| Control of Wheat Sharp Eyespot by Combination of Bacillus subtilis Z-14 Spore Preparation and Kuras |
| ZHANG Jing-Jing, WANG Min, ZHAO Gang-Yi, BIAN Yin-Bo, LIU Na, ZHANG Dong-Dong, HOU Chun-Yan, HAN Sheng-Fang*, WANG Dong-Mei* |
| State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University, Baoding 071001, China |
|
|
|
|
Abstract In recent years, the occurrence of wheat sharp eyespot has been increasing year by year, seriously affects food security. The prevention and control of wheat (Triticum aestivum) diseases in production mainly relies on chemical pesticides, but chemical control has many problems. In this paper, plate antagonism test, virulence assay and compatibility assay were used to preliminarily analyze the inhibition effect of fermentation broth of Bacillus subtilis Z-14 strain on the mycelial growth of Rhizoctonia cerealis, the virulence of pesticide Kuras on Rhizoctonia cerealis and the compatibility between Kuras and Z-14 strain. On this basis, the spore preparation of strain Z-14 (8.5×107 CFU/mL) combined with pesticide Kuras seed dressing on wheat sharp blight control was studied. The results showed that Kuras had high virulence against Rhizoctonia cerealis, and its average concentration for 50% of maximal effect (EC50) value of inhibiting the mycelial growth of Rhizoctonia cerealis was 0.035 μg/mL; At this concentration, there was no obvious inhibition on the growth of Z-14 bacteria, and it had good compatibility; The inhibition rate of Z-14 fermentation liquid on mycelia growth of wheat sharp eyespot was 86.57%. In greenhouse, the combined seed dressing of Z-14 spore preparation and Kuras had a control effect of 46.12% against sharp blight at the jointing stage of wheat, which was significantly higher than that of the single component. The combined use also promoted growth indicators such as root length, plant height, and stem thickness. Therefore, the use of biological agents to control wheat diseases is effective, it is of great significance for promoting the green development of agriculture.
|
|
Received: 21 April 2022
|
|
|
|
Corresponding Authors:
*hansf123@163.com; dongmeiwang63@126.com
|
|
|
|
[1] 陈玉琪. 2020. 微生物生长曲线中生长量测定方法的比较[J]. 轻工科技, 36(04): 7-8; 12.
(Chen Y Q.2020. Comparison of growth measurement methods in microbial growth curves[J]. Light Industry Science and Technology, 36(04): 7-8; 12. )
[2] 陈志谊, 任海英, 刘永锋, 等. 2002. 戊唑醇和枯草芽孢杆菌协同作用防治蚕豆枯萎病及增效机理初探[J]. 农药学学报, 4(4):40-44.
(Chen Z Y, Ren H Y, Liu Y F, et al.2002. The synergistic effect of tebuconazole and Bacillus subtilis to control fusarium wilt of broad bean and its synergistic mechanism[J]. Chinese Journal of Pesticides, 4(4): 40-44.)
[3] 程洁. 2021. 小麦纹枯病的发生及防治技术[J].安徽农学通报, 27(04): 97; 131 (Cheng J. 2021. Occurrence and control technology of wheat sharp eyespot [J]. Anhui Agricultural Science Bulletin, 27(04): 97; 131.)
[4] 范吉斌, 周奋启, 耿跃, 等. 2021. 不同药剂对小麦纹枯病及杂草的防效研究[J]. 现代农业科技,(22):71-73; 76.73; 76.)
[5] 高祯蔚. 2020. 两种产芽孢生防细菌的活性测定与其混合培养效果分析[D]. 硕士毕业论文, 山东农业大学, 导师: 刘振宁, pp. 17.
(Gao Z W.2020. Determination of the activity of two spore-forming biocontrol bacteria and analysis of their mixed culture effects[D]. Thesis for M.S., Shandong Agricultural University, Supervisor: Liu Z N, pp. 17.)
[6] 吴新平, 史建荣, 刘乃炽, 等. 2004. GB /T 1798. 108—2004, 农药田间药效试验准则(二)第108部分: 杀菌剂防治小麦纹枯病[S]. 北京: 中华人民共和国国家质量监督检验检疫总局.
(Wu X P, Shi J R, Liu N Z, et al.2004. GB /T 17980. 108-2004, Pesticide-Guidelines for the field efficacy trials (Ⅱ)-Part 108: Fungicides against sharp eyespot of wheat[S]. Beijing: General Administration of Quality Supervision, Inspection and Quarantine of the Peoples Republic of China.)
[7] 贺振宁, 李海燕, 苏媛, 等. 2015. 枯草芽孢杆菌B1514可湿性粉剂的研制及其对小麦纹枯病的防效[J]. 河南农业科学, 44(11): 67-72.
( He Z N, Li H Y, Su Y, et al.2015. Development of Bacillus subtilis B1514 wettable powder and its control effect on wheat sharp eyespot[J]. Henan Agricultural Science, 44(11): 67-72.)
[8] 纪兆林, 凌筝, 张清霞, 等. 2008. 地衣芽孢杆菌对苹果轮纹病菌和炭疽病菌的抑制及其对贮藏期苹果轮纹病的防治作用[J]. 果树学报, 25(2): 209-214.
(Ji Z L, Ling Z, Zhang Q X, et al.2008. Inhibition of Bacillus licheniformis on apple ringworm and anthracnose and its preventive effect on apple ringworm during storage[J]. Journal of Fruit Trees, 25(2): 209-214.)
[9] 马淑梅. 2018. 农作物主要病害诊断与防治[M]. 黑龙江大学出版社, 黑龙江, pp. 69-71.
(Ma S M.2018. Diagnosis and Control of Main Crop Diseases[M]. Heilongjiang University Press, Heilongjiang, China, pp. 69-71.)
[10] 慕立义. 1994. 植物化学保护研究方法植保专业用[M]. 北京: 中国农业出版社, pp. 76-81.
(Mu L Y.1994. Research methods of plant chemical protection for professional use in plant protection[M]. China Agricultural Press, Beijing, China, pp. 76-81.)
[11] 田书鑫, 刘南南, 王桂清. 2019. 对峙培养法在生防菌抑制效果研究中的应用[J]. 河南农业科学, 48(8): 1-6.
(Tian S X, Liu N N, Wang G Q.2019. The application of the confrontation culture method in the research of the inhibitory effect of biocontrol bacteria[J]. Henan Agricultural Science, 48(8): 1-6.)
[12] 肖茜, 闫翠梅, 齐永志, 等. 2020. 小麦纹枯病化学和生物防治研究进展[J].农药, 59(09): 630-635; 679.
(Xiao X, Yan C M, Qi Y Z, et al.2020. Research progress on chemical and biological control of wheat sharp eyespot[J]. Pesticides, 59(09): 630-635; 679.)
[13] 谢立, 贺春萍, 梁艳琼, 等. 2020. 枯草芽孢杆菌Czk1与化学杀菌剂复配对橡胶树炭疽病菌的抑菌活性[J]. 南方农业学报, 51(10): 8.
(Xie L, He C P, Liang Y Q, et al.2020. Antibacterial activity of Bacillus subtilis Czk1 combined with chemical fungicides against rubber tree anthracnose[J]. Southern Agricultural Journal, 51(10):8.)
[14] 游景茂. 2010. 芽孢杆菌WJ-1的鉴定及其对水稻纹枯病和油菜菌核病的防治研究[D]. 硕士学位论文, 华中农业大学, 导师: 姜道宏, pp. 25-26.
(You J M.2010. Identification of Bacillus WJ-1 and its control of rice sharp eyespot and rape sclerotinia[D]. Thesis for M.S., Huazhong Agricultural University, Supervisor: Jiang D H, pp. 25-26.)
[15] 张洁, 朱仁胜, 王春芳, 等. 2019. 枯草芽孢杆菌在农业领域的应用研究进展[J].现代农业科技, (13): 163-170.
(Zhang J, Zhu R S, Wang C F, et al. 2019. Application and research progress of Bacillus subtilis in agriculture[J]. Modern Agricultural Science and Technology, (13): 163-170.)
[16] 张同庆. 2019. 几种不同小麦种衣剂防效试验示范[J]. 河南农业, (34): 40-42.
(Zhang T Q. 2019. Experimental demonstration of control effect of several different wheat seed coat agents[J]. Henan Agriculture, (34): 40-42.)
[17] 周亚妮. 2010. 浅谈枯草杆菌的特性及其应用[J]. 黔南民族医专学报, 23(02): 84-88.
(Zhou Y N.2010. Characteristics and application of Bacillus subtilis[J]. Journal of Qiannan Medical College for Nationalities, 23(02): 84-88.)
[18] Boukaew S, Klinmanee C, Prasertsan P.2013. Potential for the integration of biological and chemical control of sheath blight disease caused by Rhizoctonia solani on rice[J]. World Journal of Microbiology & Biotechnology, 29(10): 1885-1893.
[19] Djordje F, Ivica D, Tanja B, et al.2018. Biological control of plant pathogens by Bacillus species[J]. Journal of Biotechnology, 285: 44-55.
[20] Hamada M S, Yin Y, Chen H, et al.2011. The escalating threat of Rhizoctonia cerealis, the causal agent of sharp eyespot in wheat[J]. Pest Management Science, 67(11):1411-1419.
[21] Huang Y, Wong P.1998. Effect of Burkholderia (Pseudomonas) cepacia and soil type on the control of crown rot in wheat[J]. Plant & Soil, 203(1): 103-108.
[22] Kiewnick S, Jacobsen B J, Braun-Kiewnick A, et al.2001. Bergman J.W. integrated control of rhizoctonia crown and root rot of sugar beet with fungicides and antagonistic bacteria[J]. Plant Disease, 85(7): 718-722.
[23] Kucuk C, Kivan M, Kinaci E, et al.2007. Biological efficacy of Trichoderma Harzianum isolate to control some fungal pathogens of wheat (Triticum aestivum) in Turkey[J]. Biologia, 62(3): 283-286.
[24] Li, S D, Chen C J, Di P, et al.2014. Combined application of Bacillus subtilis NJ-18 with fungicides for control of sharp eyespot of wheat[J]. Biological Control: Theory and Application in Pest Management, 70 : 28-34.
[25] Mcmillan V E, Canning G, Moughan J, et al.2018. Exploring the resilience of wheat crops grown in short rotations through minimising the build-up of an important soil-borne fungal pathogen[J]. Scientific Reports, 8(1): 9550.
[26] Storek K M, Chan J, Vij R, et al.2019. Massive antibody discovery used to probe structure-function relationships of the essential outer membrane protein LptD[J]. eLife Sciences, 8: e46258
[27] Zoia A A, Amna S.2019. Combating early blight infection by employing Bacillus subtilis in combination with plant fertilizers[J]. Current Plant Biology, 20(C): 100125.
[28] Zhang X C, Chen X M, Qiao X L, et al.2020. Isolation and yield optimization of lipopeptides from Bacillus subtilis Z-14 active against wheat take-all caused by Gaeumannomyces graminis var. tritici[J]. Journal of Separation Science, 44(4): 931-940. |
|
|
|
