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| Identification and Expression Analysis of WRKY Gene Family in Litsea cubeba |
| SU Wen-Juan, CHEN Xia, CAO Rui-Lan, CHEN Shang-Xing, LIU Zhou-Ying, LIU Juan* |
| Jiangxi Provincial key Laboratory of Silviculture/College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China |
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Abstract Litsea cubeba is one of the important woody spice species in southern China and is extremely susceptible to biotic and abiotic stresses during growth process. WRKY transcription factors play important roles in plant growth and development, stress response and other physiological processes. In this study, 41 LcWRKY genes were identified based on bioinformatics analysis, sorted into lines and explored for their expression specificity in different tissues. Phylogenetic development revealed that members of the LcWRKY family were divided into 2 taxa, with the 2nd taxon containing 5 subclasses (Ⅱ-a, Ⅱ-b, Ⅱ-c, Ⅱ-d, and Ⅱ-e). The results of conserved motif and structural domain analysis of the LcWRKY genes confirmed the accuracy of the phylogenetic clustering analysis. Protein interaction network co-expression analysis showed that LcWRKY36, LcWRKY38, and LcWRKY39 were at the centre of the common thread, presumably playing key roles in the regulation of Litsea cubeba in response to disease and low phosphorus stress. Analysis of transcriptome data from different tissues revealed that 41 LcWRKY members differentially expressed in different tissues, with 10 genes, including LcWRKY1, LcWRKY3 and LcWRKY8, showing high expression in roots, with LcWRKY8 being 25-fold more expressed in roots than that in leaves, presumably playing an important role in root development and stress. In addition, the relative expression of LcWRKY29, LcWRKY10, LcWRKY15, LcWRKY22, and LcWRKY1 were higher in fruits, which were presumed to be involved in the biosynthesis of secondary metabolites such as citral in bilberry fruits. The present study provides a basis for further analysis of the functions of WRKY transcription factors in L. cubeba.
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Received: 15 September 2021
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Corresponding Authors:
*liu_juan1122@163.com
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