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| Function of Eggplant (Solanum melongena) miR171b in Resistance to Verticillium dahliae Infection |
| CHEN Min, LIN Yuan-Mi, ZHU Wen-Jiao, YANG Qing* |
| College of Life Science, Nanjing Agricultural University, Nanjing 210095, China |
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Abstract microRNA171b (miR171b) is a member of miR171 family and plays an important role in plant growth and development, response to abiotic and biological stresses. To reveal the function of miR171b in eggplant (Solanum melongena) response to Verticillium dahliae, using eggplant cultivar 'Suqi No. 1' as the test material in this study, the pri-miR171b gene was cloned and its expression vector was constructed. The miR171b transgenic eggplant plants were regenerated through Agrobacterium tumefaciens-mediated genetic transformation and analyzed their resistance to Verticillium wilt. The disease resistance analysis showed that the miR171b overexpression line was more resistant to the infection of V. dahliae with disease index of 18.5, which was about 1/3 to 1/2 of the control, while miR171b antisense inhibition lines were more sensible with disease index of 68.4 and 72.6, which were 1.5~1.6 times of the control. The quantitative analysis of pathogen internal transcribed spacer (ITS）showed that the content of V. dahliae in the vascular tissues of miR171b overexpression line was significantly lower than that in the control (P<0.05), and its content in miR171b antisense lines was significantly increased (P<0.05). Antioxidant enzyme activity analysis showed that the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) enzyme in miR171b overexpression line were higher than those in the control, while their activities were lower in the antisense lines than in the control. The above results showed that miR171b was involved in the defense process of eggplant against Verticillium wilt, and played a positive regulatory role. This study provides a theoretical basis for the breeding of eggplant cultivars with the Verticillium wilt resistance.
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Received: 24 August 2021
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Corresponding Authors:
*qyang19@njau.edu.cn
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