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| Identification of GRAS Gene Family and Its Expression Pattern Analysis Under Abiotic Stress in Vigna radiata |
| ZHANG Wen-Hui*, HE Guang-Xin, WANG Zi-Xin, ZHOU Peng, XU Hong-Yan, ZHANG Qi, DU Ji-Dao |
| Agronomy College, Heilongjiang Bayi Agricultural University, Daqing 163319, China |
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Abstract GRAS (gibberellic acid insensitive (GAI), repressor of GAI (RGA), and scarecrow (SCR)) gene family widely exists in plants and plays important role in response to stress, plant growth and development. Vigna radiata is a traditional cash crop in China, with strong resistance to adversity, and it is one of the rominent crops to improved saline-alkali soil. In this study, 58 GRAS genes in Vigna radiata were identified and bioinformatics and expression analysis under abiotic stress were carried out. The results showed that GRAS genes were unevenly distributed on chromosomes 1~11 of Vigna radiata; By phylogenetic analysis, GRAS genes were divided into 8 subfamilies (PAT1, DELLA, SCL3, LISCL, LS, SCR, SHR and HAM), GRAS proteins of the same subfamily had similar protein motifs; Genetic structure analysis showed that almost all GRAS genes in Vigna radiata had no introns, and it was highly conserved in different species; The promoter region contained abscisic acid, ethylene, methyl jasmonate response elements and stress responseelements; qPCR results showed that GRAS genes in leaves could respond to abiotic stresses, such as salt, alkali, drought and low temperature stress in different degree and the expression of GRAS genes under stress was tissue specific. PAT1 subfamily gene played an important role in response to stress. The results would provide reference for analyse the functions of GRAS family genes in stress resistance in Vigna radiata.
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Received: 04 June 2021
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Corresponding Authors:
*hhsilence@163.com
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