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| Mining and Application of SSR Molecular Markers Based of the Twelve Key Enzymes of Phenolic Synthesis Pathway in Tea Plant (Camellia sinensis) |
| PENG Jing-Ru1,2,*, TAN Ye-Wei1,2, WEN Li-Xiang1,2, ZHANG Fen1,2, HUANG Shou-Hui1,2, CHEN Jia-Xian1,2, YUAN Dong-Yin1,2, ZHAO Yuan1,2 |
1 Guangxi Subtropical Crops Research Institute, Nanning 530002, China;
2 Guangxi Subtropical Fruits Processing Research Center of Engineering Technology, Nanning 530002, China; |
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Abstract Tea polyphenols are the main secondary metabolites of tea plant (Camellia sinensis). They are closely related to the metabolism, growth and quality of tea plant. The synthesis of tea tree phenolic involves a variety of enzymes. At present, the development and application of SSR molecular marker primers for specific enzymes in the tea plant synthesis pathway are still rare. This study was based on the whole genome sequence of tea plant; 158 pairs of SSR molecular marker primers were developed based on 12 key enzyme genes and their flanking sequences of tea plant phenolic synthesis pathway. Among them, the most SSR primers were obtained from flavonoid 3'-hydroxylase gene, with 29 pairs, and the least was cinnamate 4-hydroxylasegene, with only 1 pair. Among the 158 SSR primers, the most were located in the intergenic region, followed by the intron region, upstream region and exon region, and the least were located in the 5' untranslated region. These primers had 122 repeat units, among which dinucleotide was the most, followed by trinucleotide, tetranucleotide and pentanucleotide, and hexanucleotide was the least. 68 pairs of SSR primers were selected from 158 pairs of primers for primer polymorphism screening. The genetic diversity of 72 tea germplasm resources was studied by using 13 pairs of primers with good polymorphism. A total of 105 banding type were obtained, and the duplicate samples of the same sample can be gathered together. The genetic similarity coefficient (GS) of 72 tea germplasm resources ranged from 0.812 0 to 0.984 9. The results showed that the selected primers could distinguish the germplasm resources well and had good accuracy. This study can provide a basis for core germplasm construction, new variety selection and variety identification based on phenolic substances in tea plant, and also provide a reference method for developing SSR molecular markers for specific genes.
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Received: 21 July 2021
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Corresponding Authors:
*pjru99@163.com
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