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| Effect of Silencing GbGSTU7 Gene on the Resistance to Fusarium Wilt of Gossypium barbadense |
| DENG Xiao-Juan*, LU Xiao-Shuang, CHEN Qin, ZHANG Meng-Jie, QU Yan-Ying |
| Xinjiang Agricultural University, Urumqi 830052, China |
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Abstract The GSTU7 gene encodes glutathione transferase. In the previous research, this group found out that GbGSTU7 (GenBank No. GB_A03G0575) gene responds to the process of resistance to Fusarium wilt. In order to verify the GbGSTU7 gene in the resistance of Gossypium barbadense to Fusarium wilt, the Tobacco rattle virus-mediated virus induced gene silencing system was used to silence the GbGSTU7 gene in the disease-resistant material of Gossypium barbadense '06-146', and qPCR technology was used to analyze the expression of GbGSTU7 gene. The physiological race 7 of Fusarium wilt was used to infect the the GbGSTU7 gene silenced plants to study their disease resistance and measure the glutathione peroxidase activity of Gossypium barbadense. The results showed that the silencing efficiency of GbGSTU7 gene was 74%, and the disease index for cotton Fusarium wilt increased 31.4, the soluble protein content increased by 3 times, and the activity of glutathione peroxidase decreased by 2 times, indicating that the GbGSTU7 gene played an important role in the anti-fusarium wilt reaction of Gossypium barbadense. The silencing of GbGSTU7 affected protein transport and cooperated with glutathione peroxidase to resist infection by pathogens. This study provides a reference for the in-depth study of the mechanism of the GbGSTU7 gene involved in the resistance to Fusarium wilt of Gossypium barbadense.
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Received: 30 June 2021
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Corresponding Authors:
*dengxj007@163.com
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