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农业生物技术学报  2022, Vol. 30 Issue (2): 222-235    DOI: 10.3969/j.issn.1674-7968.2022.02.002
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Identification and Analysis of Differentially Expressed MYB-related Genes in Maize (Zea mays) Under Drought Stress and Rewatering
ZHANG Peng-Yu1,2, FU Jia-Xu2, QIU Xiao2, WANG Tong-Chao1,2,*, WEI Li2,*
1 The Collaborative Innovation Center of Henan Food Crops, Zhengzhou 450002, China;
2 College of Agriculture, Henan Agricultural University, Zhengzhou 450002, China
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Abstract  MYB-related gene is a subclass of MYB transcription factors, which mainly participates in the processes of secondary metabolism, growth and development, biological and abiotic stress. In order to explore the function of MYB-related transcription factor under drought stress, in the present study, 46 differentially expressed MYB-related genes were identified from the former transcriptome results under drought-rewatering treatment and comprehensive analyses were conducted including phylogenetic tree, motif prediction, gene's structure, cis-acting elements and co-expression analysis. The results showed that 46 genes were unevenly distributed on 8 chromosomes and divided into six sub-groups by phylogenetic analysis. The mainly stress-related cis-acting elements ABRE, MBS, ARE, LTR and hormone response element TCA-element, TGACG-motif, TGA-element were identified in the promoter regions of MYB-related genes. The results of gene expression analysis showed that 9 genes were up-regulated under drought stress, down-regulated after rewatering; 16 genes were down-regulated expression under drought stress while up-regulated expression after rewatering. In addition, 9 core genes responsive to drought stress were identified by co-expression network analysis. The results of qPCR showed that ZmMYBR24, ZmMYBR37, ZmMYBR55 and ZmMYBR89 genes were up-regulated under drought stress, and then down-regulated after rewatering. However, the transcript levels of ZmMYBR41, ZmMYBR78, ZmMYBR87 and ZmMYBR97 exhibited opposite trend. These results provide insight for further functional investigation of MYB-related genes.
Key wordsZea mays      Drought stress      Re-watering      MYB-related transcription factors      Comprehensive analyses     
Received: 18 May 2021     
ZTFLH:  S513  
Corresponding Authors: *nxywtc221@163.com; weili-wtc@126.com   
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ZHANG Peng-Yu
FU Jia-Xu
QIU Xiao
WANG Tong-Chao
WEI Li
Cite this article:   
ZHANG Peng-Yu,FU Jia-Xu,QIU Xiao, et al. Identification and Analysis of Differentially Expressed MYB-related Genes in Maize (Zea mays) Under Drought Stress and Rewatering[J]. 农业生物技术学报, 2022, 30(2): 222-235.
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2022.02.002     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2022/V30/I2/222
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