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| Functional Analysis of Transcription Factor Gene TaNAC35 in the Interaction Between Wheat (Triticum aestivum) and Puccinia triticina |
| ZHAO Chen-Guang1,2,*, ZHANG Na1,2,*, WEN Xiao-Lei3, YANG Wen-Xiang1,2, ZHANG Na1,2,**, LIU Da-Qun1,2,** |
1 College of Plant Protection, Hebei Agricultural University, Baoding 071000, China;
2 Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Baoding 071000, China;
3 Hebei Normal University of Science & Technology, Qinhuangdao 066000, China |
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Abstract NAC (NAM-ATAF1/2-CUC2) proteins are plant specific transcription factors, and play important roles in regulation of growth, development and stress response in plant. In the previous studies, TaNAC35 was cloned from wheat (Triticum aestivum) near iso-geneic line TcLr3ka based on the database of transcriptome RNA-seq from wheat and Puccinia triticina (Pt) interaction, in which the expression of TaNAC35 was significantly different between inoculated and uninoculated wheat. This study isolated the genome DNA (gDNA) sequence of TaNAC35 in TcLr3ka, and compared with CDS sequence by MEGA software. The results showed 4 exons and 3 introns in the gDNA sequence of TaNAC35. The promoter elements were predicted and analyzed, and the results showed that there were various of hormone responsive elements, light responsive elements and transcription factor binding sites. Recombinant plasmid pGR107-TaNAC35-GFP was constructed and transiently transformed into tobacco (Nicotiana tabacum), which revealed that TaNAC35 localized in the nucleus and the cell membrane. qPCR analysis showed TaNCA35 was highly expressed in leaves and might be involved in the response of wheat to Pt, abscisic acid and salicylic acid. Virus-induced gene silencing (VIGS) was employed to silence part of TaNAC35 sequence, the results showed that compared with TaNAC35 non-silenced wheat (control), the growth and development of Pt on TaNAC35 silenced wheat decreased significantly, which could inhibit the infection of the pathogen. The results in this study showed that TaNAC35 played a negative regulatory role in the resistance of wheat to Pt, which provides a reference for further exploration of the role of NAC transcription factors in the interaction between wheat and Pt.
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Received: 10 May 2021
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Corresponding Authors:
**zn0318@126.com;ldq@hebau.edu.cn
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