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Functional Analysis of Transcription Factor Gene TaNAC35 in the Interaction Between Wheat (Triticum aestivum) and Puccinia triticina |
ZHAO Chen-Guang1,2,*, ZHANG Na1,2,*, WEN Xiao-Lei3, YANG Wen-Xiang1,2, ZHANG Na1,2,**, LIU Da-Qun1,2,** |
1 College of Plant Protection, Hebei Agricultural University, Baoding 071000, China;
2 Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Baoding 071000, China;
3 Hebei Normal University of Science & Technology, Qinhuangdao 066000, China |
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Abstract NAC (NAM-ATAF1/2-CUC2) proteins are plant specific transcription factors, and play important roles in regulation of growth, development and stress response in plant. In the previous studies, TaNAC35 was cloned from wheat (Triticum aestivum) near iso-geneic line TcLr3ka based on the database of transcriptome RNA-seq from wheat and Puccinia triticina (Pt) interaction, in which the expression of TaNAC35 was significantly different between inoculated and uninoculated wheat. This study isolated the genome DNA (gDNA) sequence of TaNAC35 in TcLr3ka, and compared with CDS sequence by MEGA software. The results showed 4 exons and 3 introns in the gDNA sequence of TaNAC35. The promoter elements were predicted and analyzed, and the results showed that there were various of hormone responsive elements, light responsive elements and transcription factor binding sites. Recombinant plasmid pGR107-TaNAC35-GFP was constructed and transiently transformed into tobacco (Nicotiana tabacum), which revealed that TaNAC35 localized in the nucleus and the cell membrane. qPCR analysis showed TaNCA35 was highly expressed in leaves and might be involved in the response of wheat to Pt, abscisic acid and salicylic acid. Virus-induced gene silencing (VIGS) was employed to silence part of TaNAC35 sequence, the results showed that compared with TaNAC35 non-silenced wheat (control), the growth and development of Pt on TaNAC35 silenced wheat decreased significantly, which could inhibit the infection of the pathogen. The results in this study showed that TaNAC35 played a negative regulatory role in the resistance of wheat to Pt, which provides a reference for further exploration of the role of NAC transcription factors in the interaction between wheat and Pt.
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Received: 10 May 2021
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Corresponding Authors:
**zn0318@126.com;ldq@hebau.edu.cn
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[1] 李建嫄. 2018. 基于转录组分析的小麦叶锈菌效应蛋白的筛选及功能分析[D]. 硕士学位论文, 河北农业大学, 导师: 杨文香, pp. 60-63.
( Li J Y.2018. Screening and functional analysis of effector proteins based on Puccinia triticina transcriptome[D]. Thesis for M.S., Hebei Agricultural University, Supervisor: Yang W X, pp. 60-63.)
[2] 王芳, 孙立娇, 赵晓宇, 等. 2019. 植物NAC转录因子的研究进展[J]. 生物技术通报, 35(4): 88-93.
(Wang F, Sun L J, Zhao X Y, et al.2019. Research progresses on plant NAC transcription factors[J]. Biotechnology Bulletin, 35(4): 88-93.)
[3] 王瑞芳, 胡银松, 高文蕊, 等. 2014. 植物NAC转录因子家族在抗逆响应中的功能[J]. 植物生理学报, 50(10): 1494-1500.
(Wang R F, Hu Y S, Gao W R, et al.2014. Functions of NAC transcription factors family in stress responses in plants[J]. Plant Physiology Journal, 50(10): 1494-1500.)
[4] 张悦. 2020. 小麦叶锈菌效应蛋白Pt2567的特性及功能分析[D]. 硕士学位论文, 河北农业大学, 导师: 杨文香, pp. 17.
(Zhang Y.2020. Characters and functional analysis of effector protein Pt2567 in Puccinia triticina[D]. Thesis for M.S., Hebei Agricultural University, Supervisor: Yang W X, pp. 17.)
[5] Feng H, Duan X, Zhang Q, et al.2014. The target gene of tae-miR164, a novel NAC transcription factor from the NAM subfamily, negatively regulates resistance of wheat to stripe rust[J]. Molecular Plant Pathology, 15(3): 284-296.
[6] Fujita M, Fujita Y, Maruyama K, et al.2004. A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway[J]. Plant Journal, 39(6): 863-876.
[7] Hu H, You J, Fang Y, et al.2010. Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice[J]. Plant Molecular Biology, 72(4-5): 567-568.
[8] Huerta-Espino J, Singh R P, Germán S, et al.2011. Global status of wheat leaf rust caused by Puccinia triticina[J]. Euphytica, 179(1): 143-160.
[9] Kou X H, Zhou J Q, Wu C E, et al.2021. The interplay between ABA/ethylene and NAC TFs in tomato fruit ripening: A review[J]. Plant Molecular Biology, 106(3):223-238.
[10] Li J Y, Wang X D, Zhang L R, et al.2017. A wheat NBS-LRR gene TaRGA19 participates in Lr19-mediated resistance to Puccinia triticina[J]. Plant Physiology and Biochemistry, 119: 1-8.
[11] Liu Q, Yan S J, Huang W J, et al.2018. NAC transcription factor ONAC066 positively regulates disease resistance by suppressing the ABA signaling pathway in rice[J]. Plant Molecular Biology, 98(4-5): 289-302.
[12] Livak K J, Schmittgen T D.2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 25(4): 402-408.
[13] Mei F M, Chen B, Li F, et al.2021. Overexpression of the wheat NAC transcription factor TaSNAC4-3A gene confers drought tolerance in transgenic Arabidopsis[J]. Plant Physiology and Biochemistry, 160(3): 37-50.
[14] Mergby D, Hanin M, Saidi M N.2021. The durum wheat NAC transcription factor TtNAC2A enhances drought stress tolerance in Arabidopsis[J]. Environmental and Experimental Botany. DOI: 10.1016/j.envexpbot.2021.104439.
[15] Mitsuda N, Ohme-Takagi M.2009. Functional analysis of transcription factors in Arabidopsis[J]. Plant and Cell Physiology, 50(7): 1232-1248.
[16] Nakano Y, Yamaguchi M, Endo H, et al.2015. NAC-MYB-based transcriptional regulation of secondary cell wall biosynthesis in land plants[J]. Frontier in Plant Science, 6(5): 288.
[17] Ren Y, Huang Z Q, Jiang H, et al.2021. A heat stress responsive NAC transcription factor heterodimer plays key roles in rice grain filling[J]. Journal of Experimental Botany, 72(8): 2947-2964.
[18] Shan W, Chen J Y, Kuang J F, et al.2016. Banana fruit NAC transcription factor MaNAC5 cooperates with MaWRKYs to enhance the expression of pathogenesis-related genes against Colletotrichum musae[J]. Molecular Plant Pathology, 17(3): 330-338.
[19] Souer E, van Houwelingen A, Kloos D, et al.1996. The no apical meristem gene of Petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries[J]. Cell, 85(2): 159-170.
[20] Sun L J, Zhang H J, Li D Y, et al.2013. Functions of rice NAC transcriptional factors, ONAC122 and ONAC131, in defense responses against Magnaporthe grisea[J]. Plant Molecular Biology, 81(1-2): 41-56.
[21] Thomma B P H J, Penninckx I A M A, Cammue B P A, et al.2001. The complexity of disease signaling in Arabidopsis[J]. Current Opinion in Immunology, 13(1): 63-68.
[22] Wang B, Wei J P, Song N, et al.2018. A novel wheat NAC transcription factor, TaNAC30, negatively regulates resistance of wheat to stripe rust[J]. Journal of Integrative Plant Biology, 60(5): 432-443.
[23] Wang F T, Lin R M, Feng J, et al.2015. TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana[J]. Frontiers in Plant Science, 6(2): 108.
[24] Wang J, Ma Z T, Tang B, et al.2021. Tartary buckwheat (Fagopyrum tataricum) NAC transcription factors FtNAC16 negatively regulates of pod cracking and salinity tolerant in Arabidopsis[J]. International Journal of Molecular Sciences, 22(6): 3197.
[25] Wang Q, Guo C, Li Z Y, et al.2021. Potato NAC transcription factor StNAC053 enhances salt and drought tolerance in transgenic Arabidopsis[J]. International Journal of Molecular Sciences, 22(5): 2568-2568.
[26] Wang Z Y, Xia Y Q, Lin S Y, et al.2018. Osa-miR164a targets OsNAC60 and negatively regulates rice immunity against the blast fungus Magnaporthe oryzae[J]. The Plant Journal, 95(5): 584-597.
[27] Xiao Q L, Wang Y Y, Li H, et al.2021. Transcription factor ZmNAC126 plays an important role in transcriptional regulation of maize starch synthesis-related genes[J]. The Crop Journal, 9(1): 192-203.
[28] Xue G P, Way H M, Richardson T, et al.2011. Overexpression of TaNAC69 leads to enhanced transcript levels of stress up-regulated genes and dehydration tolerance in bread wheat[J]. Molecular Plant, 4(4): 697-712.
[29] Yong Y B, Zhang Y, Lyu Y M.2019. A stress-responsive NAC transcription factor from Tiger lily (LlNAC2) interacts with LlDREB1 and LlZHFD4 and enhances various abiotic stress tolerance in Arabidopsis[J]. International Journal of Molecular Sciences, 20(13): 3225.
[30] Yuan X, Wang H, Cai J T, et al.2019. Rice NAC transcription factor ONAC066 functions as a positive regulator of drought and oxidative stress response[J]. BMC Plant Biology, 19(6): 278.
[31] Zhang N, Yuan S L, Zhao C G, et al.2021. TaNAC35 acts as a negative regulator for leaf rust resistance in a compatible interaction between common wheat and Puccinia triticina[J]. Molecular Genetics and Genomics, 296(6): 279-287.
[32] Zhang X M, Zhang Q, Pei C L, et al.2018. TaNAC2 is a negative regulator in the wheat-stripe rust fungus interaction at the early stage[J]. Physiological and Molecular Plant Pathology, 102(2): 144-153.
[33] Zhou W, Qian C, Li R, et al.2018. TaNAC6s are involved in the basal and broad-spectrum resistance to powdery mildew in wheat[J]. Plant Science, 277(9): 218-228. |
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