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| Sequence Characterization and Differential Expression Profiles After Starvation and Bacterial Infection of HSP70-4 in Acipenser dabryanus |
| GONG Quan, LI Qing-Zhi, SUN Jia-Hua, LIN Jue*, XIAO Yu, SU Xu-Tao, LIU Guang-Xun, DU Jun |
| Fisheries Institute, Sichuan Academy of Agricultural Sciences, Chengdu 611713, China |
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Abstract Heat shock proteins (HSPs) are highly conserved among different species, which are involved in protein folding, stabilization of biological matrix, assembly of macromolecules, degradation of polypeptides and transcriptional regulation. To study the expression profiles of HSP70-4 in different tissues or under the stress of fasting and bacteria invasion, The sequence of HSP70-4 gene were identified based on full-length transcriptome sequencing of Acipenser dabryanus. The full-length cDNA sequence of HSP70-4 was 4 029 bp, which included 234 bp of the 5'-UTR, 1 245 bp of the 3'-UTR and 2 550 bp of the ORF (GenBank No. MZ285757) that encoded 849 amino acids. Phylogenetic analysis showed that the A. dabryanus HSP70-4 gene clustered mostly close to the A. ruthenus HSP70-4, supported with a significant bootstrap value (100). HSP70-4 was universally expressed in all 11 of the tested tissues including liver, gonad, muscle, brain, eye, skin, head kidney, heart, intestine, spleen, and gill. The expression of HSP70-4 was the highest in the eye and was also detected at relatively high levels in the brain and muscle, and relatively weakly expressed in the liver, head kidney, skin, and intestine. Under starvation stress, the expression of HSP70-4 was upregulated, and significantly upregulated at 14 d post-fasting in intestine and muscle. HSP70-4 in the skin, spleen and head kidney was significantly upregulated (P<0.05) at 12 h post-challenge towards Edwardsiella tarda (1×107 CFU/mL) infection and the upregulation of HSP70-4 in the gill started at 24 h post-challenge. The results showed that short-term bacteria infection resulted in significantly upregulated expression of HSP70-4 gene in the gill, skin, spleen and kidney of A. dabryanus. This study demonstrated that HSP70-4 was involved in the biological process of fighting hunger and infection by pathogenic bacteria in A. dabryanus. This study demonstrated that HSP70-4 was involved in the biological process of fighting hunger and infection by pathogenic bacteria and provided a theoretical basis for the further study on the mechanism of induced differential expression of HSP70-4 gene and the stress resistance mechanism of A. dabryanus.
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Received: 02 January 2021
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Corresponding Authors:
* linjue1030@sina.com
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