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| Genome-wide Identification and Expression Analysis of NCED Gene Family in Phyllostachys edulis |
| XU Yin1, HU Qiu-Tao1, HOU Dan1, LU Hai-Wen1, LIN Xin-Chun1,2,* |
1 State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China;
2 Zhejiang Provincial Collaborative Innovation Center for Bamboo Resources and High-efficiency Utilization, Hangzhou, 311300, China |
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Abstract 9-cis-epoxycarotenoid dioxygenase (NCED) is the key rate-limiting enzyme in abscisic acid (ABA) biosynthesis pathway. In this study, bioinformatics method was used to identify 10 genes with complete NCED conserved domain RPE65 from the whole genome of Phyllostachys edulis. The length of the NCED proteins ranged from 240 to 654 aa with molecular mass of 28.03 to 70.04 kD. Phylogenetic analysis showed that the 10 NCED genes could be divided into 2 subgroups, and most NCED genes were closely related to rice (Oryza sativa) NCED genes, except for PH02Gene49300 and PH02Gene41427. The predictive analysis of cis-acting elements showed that NCED gene promoter contained a large number of abiotic stress response elements, indicating that they might play an important role in the stress resistance of Ph. edulis. Transcriptome data analysis showed that the expression of NCED genes in different tissues and developmental stages of Ph. edulis was specific, especially highly expressed in the roots and shoots (P<0.05). The qRT-PCR results showed that NCED genes had different expression patterns under ABA, salicylic acid (SA), drought and salt stress treatments, which suggested that NCEDs might play different roles in the regulation of stress and hormones response. The present work provides a basic data for further study of the function of NCED gene family in Phyllostachys edulis.
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Received: 27 September 2020
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Corresponding Authors:
* lxc@zafu.edu.cn
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