番茄钙调蛋白结合转录因子CAMTA家族基因的鉴定及其低温胁迫下表达模式的分析

doi:10.3969/j.issn.1674-7968.2021.05.005

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Abstract Plant calmodulin-binding transcription factor (CAMTA) plays important roles in hormone signal transduction, developmental regulation, and low-temperature stress. In this study, the genome-wide identification of CAMTA genes were carried out in tomato (Solanum lycopersicum), the pivotal information of the family members was comprehensively analyzed by bioinformatics technology, and qRT-PCR was used to analyse the expression pattern of them under low-temperature stress. The results showed that there were 18 CAMTA gene family members were identified in tomato genome, which were distributed on 9 chromosomes and existed in one pair of segmental duplication gene. Based on phylogenetic analysis, the SlCAMTA members were clustered into 6 groups. There are 49 cis-acting elements related to plant hormone, stress, light response and tissue-specific expression were identified in the promoter region. Protein-protein interaction network showed that some of SlCAMTA proteins were closely related to Arabidopsis reported proteins. Most of the SlCAMTAs were expressed in various tissues, and more than half of the CAMTA genes had molecular functions and constituted the basic components of cells. Especially, SlCAMTA10 participated in 11 plant biological processes. Under low-temperature stress, 14 SlCAMTAs were significantly up-regulated expression, and the expression level of SlCAMTA02 reached the highest at 12 h (396.53), which was 396 times as compared with the control. Accordingly, this study provides a reference for further excavation of the gene function in tomato CAMTA family and the response mechanism to low-temperature tolerance.

Key words： Tomato Calmodulin-binding transcription factor (CAMTA) Bioinformatics Low temperature stress Expression analysis

Received: 10 November 2020 Published: 01 May 2021

ZTFLH:

S641.2

Corresponding Authors: *weixh@gsau.edu.cn

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	WANG Bao-Qiang
	ZHU Xiao-Lin
	WEI Xiao-Hong
	JIN Bao-Xia
	WANG Xian
	WANG Wang-Tian

Cite this article:

WANG Bao-Qiang,ZHU Xiao-Lin,WEI Xiao-Hong, et al. Identification of Calmodulin-binding Transcription Factor CAMTA Gene Family and Its Expression Analysis Under Low-temperature Stress in Tomato (Solanum lycopersicum)[J]. 农业生物技术学报, 2021, 29(5): 871-884.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.05.005 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I5/871

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