Physiological Changes and Related Genes Expression Analysis of Sorghum (Sorghum bicolor) Seedlings Under Low Temperature Stress
SHAO Wen-Jing, ZHANG Jin-Jie, GE Sheng-Nan, WEI Yu-Lei, LI Jia-Xin, WU Geng-Jin, LIU Xin-Yu, HE Lin, WEI Jin-Peng*, XU Jing-Yu*
Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, Heilongjiang Engineering Technology Research Center for Crop Straw Utilization, Heilongjiang Province Cultivating Collaborative Innovation Center for The Beidahuang Modern Agricultural Industry Technology, National Coarse Cereal Engineering Research Center, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Abstract Low temperature is one of the critical environmental stress affecting the growth and development of sorghum (Sorghum bicolor) in the cold region. In order to study the physiological changes of sorghum seedlings leaves and the expression of related genes under low temperature stress, the cold-resistance variety 'P61' and the cold-sensitive variety 'H21' were selected as the test materials, which were subjected to low-temperature treatment at 4 ℃ for 0, 1, 3, 5 and 7 d, respectively. The results showed that the chlorophyll fluorescence parameters (Fv/Fm) and relative chlorophyll content (SPAD) decreased significantly with the extension of the low-temperature treatment time, whereas the decrease was not obvious in the cold-tolerant variety 'P61'; the expression of genes related to photosynthesis were down-regulated with the prolonged stress time, and the expression level was higher in resistant varieties; the activity of superoxide dismutase (SOD) and peroxidase (POD) was elevated after 5~7 d treatment. At 7 d, the SOD activity of 'H21' decreased by 3.3%, the SOD activity of 'P61' increased by 15.2%, and the POD activity of 'H21' and 'P61' increased by 7.4% and 9%, respectively. The content of malondialdehyde (MDA) and proline (Pro) increased. The MDA content of 'P61' and 'H21' increased by 36.5% and 1.7%, respectively, the content of Pro increased by 69% and 230.5%. A set of 8 SbSODs genes were screened from transcriptome database, the number of SbSODs up-regulated at low temperature for 3 d were larger than those treated for 1 d, and the expression of SbSOD5 was up-regulated in the low-temperature treatment of both varieties for 1 d and 3 d; 7 sorghum PODs genes were screened, and the most of the genes (5 out of 7) were up-regulated in both varieties when treated for 1 d, Sobic.009G055100 was up-regulated in both varieties after 1 d and 3 d treatment; a total of 11 SbLOXs were screened, and a number of up-regulated SbLOXs (lipoxygenase) were observed at 1 d time point, and SbLOX9 was up-regulated in both varieties under 1 d and 3 d treatments; 2 sorghum P5CS genes were selected, of which Sobic.003G356000 was significantly up-regulated at different time points in two varieties, and a high expression level (Log2FC>6 ) was detected in 'P61' when treated for 3 d. This study provides a reference for further research on the physiological regulation of plants under low temperature stress and the transcriptional regulation of related genes.
SHAO Wen-Jing,ZHANG Jin-Jie,GE Sheng-Nan, et al. Physiological Changes and Related Genes Expression Analysis of Sorghum (Sorghum bicolor) Seedlings Under Low Temperature Stress[J]. 农业生物技术学报, 2021, 29(5): 857-870.
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