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| Transcriptome-wide Profiling of the TCP Gene Family in Lagerstroemia indica |
| ZHANG Ye, LIU Jie-Ru, FENG Lu, ZHOU Yang, CAI Ming, CHENG Tang-Ren, WANG Jia, ZHANG Qi-Xiang, PAN Hui-Tang* |
| Beijing Forestry University, College of Landscape Architecture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Beijing 100083, China |
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Abstract TCP (teosinte branched 1, cycloidea, proliferating cell factors) transcription factors are widely involved in plants, which play an important role in regulating plant growth and development, morphogenesis, abiotic stress and other physiological processes as a kind of plant-specific transcription factor. They can regulate the expression of downstream target genes to transform the cell division process, and play a role in a variety of hormone regulatory pathways. Lagerstroemia indica, as an excellent kind of summer flowering woody plant, is commonly used in gardens. Because of its rich germplasm resources and diverse plant architecture, it is a good material for investigating woody plant architecture. In order to clarify the role of the TCP gene family in Lagerstroemia, a total of 44 TCP genes were identified in Lagerstroemia based on transcriptome data. The bioinformatics and gene expression analyses were used to analyze the characters and expression patterns of LfiTCPs. The results showed that the proteins encoded by the LfiTCPs were uniform and stable in physical and chemical properties, and were mostly localized in the nucleus. Phylogenetic analysis showed that the LfiTCPs were divided into 2 branches, and the number and proportion of which were similar to other species. LfiTCPs had a relatively conservative structure, which could maintain the TCP domain intact, and those LfiTCPs in 2 branches had obvious structural differences. Most of the LfiTCPs were similarly expressed in standard and dwarf crapemyrtles,14 of them were initially screened out based on the differential expression results. Among which, LfiTCP12;2 and LfiTCP15;2 showed significant differences between non-dwarf and dwarf progenies. The result of qRT-PCR confirmed that they were highly expressed and lowly expressed in dwarf progenies, respectively. This study provides the information for further research on the plant architecture of Lagerstroemia indica, potentially promoting the molecular breeding process of woody plant architecture.
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Received: 23 June 2020
Published: 01 March 2021
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Corresponding Authors:
*htpan@bjfu.edu.cn
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