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| Cloning and Expression Analysis of SsCBL3 in Sugarcane (Saccharum officinarum) |
| LING Qiu-Ping, ZENG Qiao-Ying, ZHOU Wen-Ling, AO Jun-Hua, HUANG Ying, WU Qi-Hua, SHEN Da-Chun* |
| Institute of Bioengineering, Guangdong Academy of Sciences/Guangdong Modern Agricultural Technology Research and Development Center (Resources and Environment and Agricultural Product Safety), Guangzhou 510316, China |
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Abstract Calcineurin B-Like proteins (CBLs) are a kind of important protein which can involve in decoding calcium signals in plants. They play important role in plant response to various stresses. The cDNA of SsCBL3 (GenBank. MN850491) cloned from sugarcane (Saccharum officinarum) cultivar 'ROC22' under low potassium stress by reverse transcription-PCR (RT-PCR). SsCBL3 contained a complete open reading frame of 678 bp encoding a protein with 225 amino acids. The sequence features of SsCBL3 analyzed by bioinformatics. The molecular weight of SsCBL3 was 25.767 47 KD and the isoelectric point was 4.82, which was an acidic protein. The structure of SsCBL3 protein was analyzed showed that it contained 3 EF-hand functional domains and 1 FPSF motif which interacted with CIPK kinases. Evolutionary analysis further revealed that SsCBL3 has high homology to the CBL3 from gramineous crop, and had the closest relationship with Zea may. The result of qRT-PCR showed that the expression of SsCBL3 was changed under low nitrogen, low phosphorus, low potassium, drought, salt and ABA stresses. Moreover, the different stress times also change expression of SsCBL3. The expression of SsCBL3 strongly induced by drought or salt stresses. The results of qRT-PCR indicated that SsCBL3 gene may play an important regulatory role in sugarcane under stress. The results of research provides a theoretical basis for cultivating sugarcane varieties with stress-resistant.
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Received: 28 June 2020
Published: 01 March 2021
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Corresponding Authors:
*shdach@163.com
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