牦牛<em>PTGES</em>基因克隆及其在发情周期主要生殖器官的表达定位

doi:10.3969/j.issn.1674-7968.2021.10.009

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Abstract Prostaglandin E synthase (PTGES) is the terminal rate-limiting enzyme for prostaglandin E2 (PGE2) synthesis, which catalyzes the conversion of prostaglandin H2 (PGH2) into PGE2. PGE2 can specifically bind to receptors and participate in various physiological processes, including animal reproduction. This study investigated the expression of PTGES in yak (Bos grunniens) under normal physiological conditions and its role in the reproductive process. There were 9 experimental samples: Female yaks' uterus, ovaries and fallopian tubes at different stages of the estrus cycle (follicular phase, luteal phase and pregnancy). The mRNA and protein expression of PTGES were analyzed by gene cloning, qPCR and immunohistochemistry (IHC). The results showed that the predicted proteins encoded by PTGES gene (GenBank No. MW030282) were hydrophilic non-transmembrane proteins through bioinformatics analysis. PTGES gene was expressed in yak fallopian tubes, ovaries and uterus, but there were some difference. The expression of PTGES in the ovary during the luteal phase was significantly higher than that of the follicular phase and pregnancy (P<0.05). The expression of PTGES in the fallopian tube during the follicular phase was significantly higher than that in the luteal phase and pregnancy (P<0.05). The expression of PTGES in the uterus during the follicular phase was significantly higher than that in the luteal phase and pregnancy (P<0.05). It could be known from the results of immunohistochemistry that PTGES was expressed in the luteum cells, oviduct mucosa epithelium, ovarian follicular membrane, ovarian granular layer, ovarian reproductive epithelium, endometrium, uterine glands and serosal membrane. The results of this study was speculated that PTGES might be involved in the regulation of yak reproductive physiological processes. This research provides a part of theoretical support for deeper exploration of PTGES involved in mammalian reproductive physiology regulation, which is helpful to the study of the breeding rules of mammals in the plateau.

Key words： Yak Reproduction Prostaglandin E synthetase (PTGES) Estrus cycle

Received: 08 January 2021

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S857.2+2

Corresponding Authors: * cuiyan369@sina.com

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	ZHANG Hui
	PAN Yang-Yang
	WANG Meng
	WANG Jing-Lei
	ZHANG Yan-Yan
	GAO Li-Qing
	MA Wen-Bin
	HUANG Jia-Xin
	YU Si-Jiu
	CUI Yan

Cite this article:

ZHANG Hui,PAN Yang-Yang,WANG Meng, et al. Cloning of Yak's (Bos grunniens) PTGES Gene and Its Expression and Location in Main Reproductive Organs of Estrus Cycle[J]. 农业生物技术学报, 2021, 29(10): 1949-1957.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.10.009 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I10/1949

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