|
|
|
| Analysis of Gene Expression Profiles of Rice (Oryza sativa) Monogenic Line of IRBL9-W During Its Early Stage of Resistance to Magnaporthe oryzae |
| JIANG Zhao-Yuan1, 2, REN Jin-Ping1, 2, *, LI Li1, 2, LIU Xiao-Mei1, 2, *, ZHU Feng1, 2, WANG Ji-Chun1, 2, SUN Hui1, 2 |
1 Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Changchun 130033, China;
2 Key Laboratory of Integrated Pest Management on Crops in Northeast, Ministry of Agriculture, Gongzhuling136100, China |
|
|
|
|
Abstract The rice (Oryza sativa) monogenic line of 'IRBL9-W' (containing resistance gene (Pi9)) was resistant to most of the Magnaporthe grisea isolates collected in Jilin province, indicating that the isolates contain the avirulence gene (AVR) widely spread in Jilin Providence. To investigate the gene expression profiles of the rice during the early interaction between Pi9 and avirulence gene (AVR-Pi9), the rice monogenic line of 'IRBL9-W' (Pi9) inoculated with Magnaporthe grisea isolate C2 (AVR-Pi9) in this study. The RNA-seq technology was used to analyze the expression profiles of the inoculated and uninoculated rice leaves of 'IRBL9-W' (Pi9) at 36 h after inoculation and a total of 2 311 genes were found to express differently in the two samples. The analyses of GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways showed that 3 of the 4 leucine zipper protein genes were up-regulated, 1 zinc finger protein gene was up-regulated and all the WRKY protein genes were down-regulated among transcriptional regulator genes, 2 chitinase genes were up-regulated among the10 chitinase genes and all the two β-1,3 glucanase genes were down-regulated among the fungal cell wall degrading enzyme genes, 1 pathogenesis-related protein 1 gene (PR1) was up-regulated and the other genes were down-regulated in the plant-pathogen interaction pathway, all of the differentially expressed genes were up-regulated in the flavonoid biosynthesis pathway, and 9 genes were up-regulated and 1 gene was down-regulated in the phenylpropanoid biosynthesis pathway. The differentially expressed gene products in the 3 pathways were involved in getting the reactive oxygen species, the hypersensitive response, and the antioxidant process. The functions of the compounds relating to these gene products were antioxidation, antifungal action, and lignification. This study would provide a theory to further understand the mechanism of rice blast resistance.
|
|
Received: 07 January 2020
|
|
|
|
Corresponding Authors:
* , 15043461118@163.com; xmsuliu@163.com
|
|
|
|
[1] 李彬, 王德正, 邓元宝, 等. 2014. 一个粳稻来源抗稻瘟病基因的鉴定、遗传分析和基因定位[J].作物学报, 40(1): 54-62.
(Li B, Wang D Z, Deng Y B, et al.2014. Identification, genetic analysis and gene m apping of a rice blast resistance gene in japonica rice[J]. Acta Agronomica Sinica, 40(1): 54-62.)
[2] Boisard S, Le Ray A M, Landreau A, et al. 2015. Antifungal and antibacterial metabolites from a French poplar type propolis[J]. Evidence-based Complementary and Alternative Medicine, 2015: 319240-319240.
[3] Boulogne I, Petit P, Ozier-Lafontaine H, et al.2012. Insecticidal and antifungal chemicals produced by plants: A review[J]. Environmental Chemistry Letters, 10(4): 325-347.
[4] Chujo T, Miyamoto K, Shimogawa T, et al.2013. OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate immune responses in rice against rice blast fungus[J]. Plant Molecular Biology, 82(1-2): 23-37.
[5] Dao T T, Linthorst H J, Verpoorte R.2011. Chalcone synthase and its functions in plant resistance[J]. Phytochemistry Reviews, 10(3): 397-412.
[6] Feng H L, Tian L, Chai W M, et al.2014. Isolation and purification of condensed tannins from flamboyant tree and their antioxidant and antityrosinase activities[J]. Applied Biochemistry and Biotechnology, 173(1): 179-192.
[7] Flor H H.1971. Current status of the gene-for-gene concept[J]. Annual Review of Phytopathology, 9(1): 275-296.
[8] Gupta S K, Rai A K, Kanwar S S, et al.2012. Comparative analysis of zinc finger proteins involved in plant disease resistance[J]. PLOS ONE, 7(8): e42578.
[9] Jia C, Zhang J, Yu L, et al.2019. Antifungal activity of coumarin against candida albicans is related to apoptosis[J]. Frontiers in Cellular and Infection Microbiology, 8: 445.
[10] Jin Y S.2019. Recent advances in natural antifungal flavonoids and their derivatives[J]. Bioorganic & Medicinal Chemistry Letters, 29(19): 126589.
[11] Kanzaki, Saitoh, Ito, et al.2003. Cytosolic HSP90 and HSP70 are essential components of INF1-mediated hypersensitive response and non-host resistance to Pseudomonas cichorii in Nicotiana benthamiana[J]. Molecular Plant Pathology, 4(5): 383-391.
[12] Katsumata S, Hamana K, Horie K, et al.2017. Identification of sternbin and naringenin as detoxified metabolites from the rice flavanone phytoalexin sakuranetin by pyricularia oryzae[J]. Chemistry & Biodiversity, 14(2): e1600240.
[13] Li B B, Liu Y G, Wu T, et al.2019. OsBGLU19 and OsBGLU23 regulate disease resistance to bacterial leaf streak in rice[J]. Journal of Integrative Agriculture, 18(6): 1199-1210.
[14] Liu H, Guo Z, Gu F, et al.2017. 4-coumarate-coa ligase-like gene OsAAE3 negatively mediates the rice blast resistance, floret development and lignin biosynthesis[J]. Frontiers in Plant Science, 7(1): 2041.
[15] Liu T, Li Z, Li R, et al.2019. Composition analysis and antioxidant activities of the Rhus typhina L. stem[J]. Journal of Pharmaceutical Analysis, 9(5): 332-338.
[16] Liu W, Liu J, Ning Y, et al.2013. Recent progress in understanding PAMP-and effector-triggered immunity against the rice blast fungus Magnaporthe oryzae[J]. Molecular Plant, 6(3): 605-620.
[17] Lou H, Jing X, Ren D, et al.2012. Eriodictyol protects against H2O2-induced neuron-like PC12 cell death through activation of Nrf2/ARE signaling pathway[J]. Neurochemistry International, 61(2): 251-257.
[18] Marques R, Maia C J, Vaz C, et al.2014. The diverse roles of calcium-binding protein regucalcin in cell biology: From tissue expression and signalling to disease[J]. Cellular and Molecular Life Sciences, 71(1): 93-111.
[19] Mersmann S, Bourdais G, Rietz S, et al.2010. Ethylene signaling regulates accumulation of the fls2 receptor and is required for the oxidative burst contributing to plant immunity[J]. Plant Physiology, 154(1): 391-400.
[20] Mutuku J M, Cui S, Hori C, et al.2019. The structural integrity of lignin is crucial for resistance against <em>Striga hermonthica & lt; /em> Parasitism in Rice[J]. Plant Physiology, 179(4): 1796.
[21] Nalley L, Tsiboe F, Durand-Morat A, et al.2016. Economic and environmental impact of rice blast pathogen (Magnaporthe oryzae) alleviation in the United States[J]. PLOS ONE, 11(12): e0167295.
[22] Neill S, Barros R, Bright J, et al.2008. Nitric oxide, stomatal closure, and abiotic stress[J]. Journal of Experimental Botany, 59(2): 165-176.
[23] Neng H, Xiaorong T, Kuntai L, et al.2017. Changes in protein content and chitinase and β-1, 3-glucanase activities of rice with blast resistance induced by ag-antibiotic 702[J]. Plant Diseases and Pests, 8(4): 33-6.
[24] Noman A, Liu Z, Aqeel M, et al.2017. Basic leucine zipper domain transcription factors: The vanguards in plant immunity[J]. Biotechnology Letters, 39(12): 1779-1791.
[25] Noorbakhsh Z, Taheri P.2016. Nitric oxide: A signaling molecule which activates cell wall-associated defense of tomato against Rhizoctonia solani[J]. European Journal of Plant Pathology, 144(3): 551-568.
[26] OuYang Q, Duan X, Li L, et al.2019. Cinnamaldehyde exerts its antifungal activity by disrupting the cell wall integrity of geotrichum citri-aurantii[J]. Front Microbiol, 10: 55.
[27] Parvin K, Hasanuzzaman M, Bhuyan M, et al.2019. Quercetin mediated salt tolerance in tomato through the enhancement of plant antioxidant defense and glyoxalase systems[J]. Plants, 8(8): 247-267.
[28] Ramirez J, Cartuche L, Morocho V, et al.2013. Antifungal activity of raw extract and flavanons isolated from Piper ecuadorense from Ecuador[J]. Revista Brasileira de Farmacognosia, 23(2): 370-373.
[29] Romualdi C, Bortoluzzi S, D'Alessi F, et al.2003. IDEG6: A web tool for detection of differentially expressed genes in multiple tag sampling experiments[J]. Physiol Genomics, 12: 159-62.
[30] Shreaz S, Bhatia R, Khan N, et al.2013. Influences of cinnamic aldehydes on H(+) extrusion activity and ultrastructure of Candida[J]. Journal of Medical Microbiology, 62(Pt 2): 232-240.
[31] Stange R R, Alessandro R, Greg Mc Collum T, et al.2002. Studies on the phloroglucinol-HCl reactive material produced by squash fruit elicited with pectinase: Isolation using hydrolytic enzymes and release of p-coumaryl aldehyde by water reflux[J]. Physiological and Molecular Plant Pathology, 60(6): 283-291.
[32] Suzuki S, Ma J F, Yamamoto N, et al.2012. Silicon deficiency promotes lignin accumulation in rice[J]. Plant Biotechnology, 29(4): 391-394.
[33] Talbot N J.2003. On the trail of a cereal killer: Exploring the biology of Magnaporthe grisea[J]. Annual Review of Microbiology, 57(1): 177-202.
[34] Terpinc P, AbramoviČ H.2016. Oljna pogača navadnega rička (Camelina sativa (L.) Crantz)-neizkoriščeni vir fenolnih spojin[J]. Acta agriculturae Slovenica, 107(1): 243.
[35] Tomar A, Vasisth S, Khan S I, et al.2017. Galangin ameliorates cisplatin induced nephrotoxicity in vivo by modulation of oxidative stress, apoptosis and inflammation through interplay of MAPK signaling cascade[J]. Phytomedicine, 34(2017): 154-161.
[36] Tonnessen B W, Manosalva P, Lang J M, et al.2015. Rice phenylalanine ammonia-lyase gene OsPAL4 is associated with broad spectrum disease resistance[J]. Plant Molecular Biology, 87(3): 273-286.
[37] Vencio, RZ, Brentani H,Pereira CA, et al.2003. Using credibility intervals instead of hypothesis tests in SAGE analysis[J]. Bioinformatics,19: 461-2464.
[38] Wang B H, Ebbole D J, Wang Z H, et al.2017. The arms race between Magnaporthe oryzae and rice: Diversity and interaction of Avr and R genes[J]. Journal of Integrative Agriculture, 16(12): 2746-2760
[39] Wang S B, Jang J Y, Chae Y H, et al.2015. Kaempferol suppresses collagen-induced platelet activation by inhibiting NADPH oxidase and protecting SHP-2 from oxidative inactivation[J]. Free Radical Biology and Medicine, 83: 41-53.
[40] Wang X G, Wei X Y, Tian Y Q, et al.2010. Antifungal Flavonoids from Ficus sarmentosa var. henryi (King) Corner[J]. Agricultural Sciences in China, 9(5): 690-694.
[41] Wang Y, Feng K, Yang H, et al.2018. Antifungal mechanism of cinnamaldehyde and citral combination against Penicillium expansum based on FT-IR fingerprint, plasma membrane, oxidative stress and volatile profile[J]. RSC Advances, 8(11): 5806-5815.
[42] Wang Y, Sun Y, Wang J, Zhou M, et al.2019. Antifungal activity and action mechanism of the natural product cinnamic acid against Sclerotinia sclerotiorum[J]. Plant Disease, 103(5): 944-950.
[43] Yan J, Yuan S S, Jiang L l, et al.2015. Plant antifungal proteins and their applications in agriculture[J]. Applied Microbiology and Biotechnology, 99(12): 4961-4981.
[44] Zhang R, Lee I K, Piao M J, et al.2011. Butin (7,3′,4′-Trihydroxydihydroflavone) reduces oxidative stress-induced cell death via inhibition of the mitochondria-dependent apoptotic pathway[J]. International Journal of Molecular Sciences, 12(6): 3871-3887. |
|
|
|
