Characteristics and Thermotolerance Regulating Role of Heat Shock Transcription Factor Gene TaHsfA2-12 in Wheat (Triticum aestivum)
YUAN Sai-Nan1,2,*, HU Dong1,*, LIU Chang3, PENG Yi-Feng4, ZHANG Hua-Ning1, ZHANG Yu-Jie1, ZHANG Yuan-Yuan1, YU Xue-Chao1, LI Guo-Liang1,*, GUO Xiu-Lin1,**
1 Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China; 2 College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China; 3 College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; 4 Shijiazhuang Academy of Agriculture and Forestry Science, Shijiazhuang 050000, China
Abstract Plant heat shock transcription factors (Hsfs) plays a central role in thermotolerance, transgenerational thermomemory and many stress responsive processes. In this paper, a new gene TaHsfA2-12 (GenBank No. MK931301) was isolated from young leaves of wheat (Triticum aestivum) treated under heat shock (HS) at 37 ℃ for 1.5 h through homologous clone technique. The CDS length of gene TaHsfA2-12 was 1 134 bp encoding 377 amino acids residues. The amino acid sequence of TaHsfA2-12 contained a DNA-binding domain (DBD), a nuclear localization signal of RRKELAEALLSKKRGR peptide, a nuclear export signal of LLSLGLE peptide and an aromatic, large hydrophobic and acidic amino residues of ESFWKELLSL peptide. The TaHsfA2-12 protein shared 98% identities with the AtHsfA2 from Aegilops tauschii. TaHsfA2-12 protein was dyed with 4',6-diamidino-2-phenylindole (DAPI) and observed in tobacco (Nicotiana tabacum) epidermal cells under Confocal, the result revealed that the TaHsfA2-12 was localized in the nuclei under the normal conditions. qRT-PCR revealed that the gene TaHsfA2-12 constitutively expressed in different tissues of wheat under the normal conditions, such as young and mature root, shoot, leaf and pistil, stamen, sepal and embryos. The TaHsfA2-12 expression levels were lower in majority of tissues and higher in mature seed (P<0.05). TaHsfA2-12 was obviously up-regulated by heat shock (HS) and 20% PEG6000, but down-regulated by H2O2 and salicylic acid (SA), respectively. Phenotypes observation indicated that TaHsfA2-12 not only improved the basal thermotolerance and acquired thermotolerance of transgenic Arabidopsis seedlings, but also could rescue the thermotolerance phenotype defect of AtHsfA2 deletion mutant under HS. Chlorophyll content of different lines were consistent with phenotypes. TaHsfA2-12 could activate a suite of heat shock protein (Hsp) genes expression in transgenic Arabidopsis plants after different HS, suggesting molecular mechanism of regulating thermotolerances of TaHsfA2-12. These results will be benefited to deeply investigate the biological characteristics and functions of wheat Hsfs family members.
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