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| Characteristics and Thermotolerance Regulating Role of Heat Shock Transcription Factor Gene TaHsfA2-12 in Wheat (Triticum aestivum) |
| YUAN Sai-Nan1,2,*, HU Dong1,*, LIU Chang3, PENG Yi-Feng4, ZHANG Hua-Ning1, ZHANG Yu-Jie1, ZHANG Yuan-Yuan1, YU Xue-Chao1, LI Guo-Liang1,*, GUO Xiu-Lin1,** |
1 Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China;
2 College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China;
3 College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;
4 Shijiazhuang Academy of Agriculture and Forestry Science, Shijiazhuang 050000, China |
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Abstract Plant heat shock transcription factors (Hsfs) plays a central role in thermotolerance, transgenerational thermomemory and many stress responsive processes. In this paper, a new gene TaHsfA2-12 (GenBank No. MK931301) was isolated from young leaves of wheat (Triticum aestivum) treated under heat shock (HS) at 37 ℃ for 1.5 h through homologous clone technique. The CDS length of gene TaHsfA2-12 was 1 134 bp encoding 377 amino acids residues. The amino acid sequence of TaHsfA2-12 contained a DNA-binding domain (DBD), a nuclear localization signal of RRKELAEALLSKKRGR peptide, a nuclear export signal of LLSLGLE peptide and an aromatic, large hydrophobic and acidic amino residues of ESFWKELLSL peptide. The TaHsfA2-12 protein shared 98% identities with the AtHsfA2 from Aegilops tauschii. TaHsfA2-12 protein was dyed with 4',6-diamidino-2-phenylindole (DAPI) and observed in tobacco (Nicotiana tabacum) epidermal cells under Confocal, the result revealed that the TaHsfA2-12 was localized in the nuclei under the normal conditions. qRT-PCR revealed that the gene TaHsfA2-12 constitutively expressed in different tissues of wheat under the normal conditions, such as young and mature root, shoot, leaf and pistil, stamen, sepal and embryos. The TaHsfA2-12 expression levels were lower in majority of tissues and higher in mature seed (P<0.05). TaHsfA2-12 was obviously up-regulated by heat shock (HS) and 20% PEG6000, but down-regulated by H2O2 and salicylic acid (SA), respectively. Phenotypes observation indicated that TaHsfA2-12 not only improved the basal thermotolerance and acquired thermotolerance of transgenic Arabidopsis seedlings, but also could rescue the thermotolerance phenotype defect of AtHsfA2 deletion mutant under HS. Chlorophyll content of different lines were consistent with phenotypes. TaHsfA2-12 could activate a suite of heat shock protein (Hsp) genes expression in transgenic Arabidopsis plants after different HS, suggesting molecular mechanism of regulating thermotolerances of TaHsfA2-12. These results will be benefited to deeply investigate the biological characteristics and functions of wheat Hsfs family members.
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Received: 24 December 2019
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Corresponding Authors:
** myhf2002@163.com; Guolianglili@163.com
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| About author:: * The authors who contribute equally |
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[1] 李春光, 陈其军, 高新起, 等. 2005. 拟南芥热激转录因子AtHsfA2调节胁迫反应基因的表达并提高热和氧化胁迫耐性[J]. 中国科学(生命科学), 035(005): 398-407.
[2] 李慧聪, 李国良, 郭秀林. 2015. 玉米热激转录因子基因(ZmHsf06)的克隆、表达和定位分析[J]. 农业生物技术学报, 23(1): 41-51.
(Li H C, Li G L, Guo X L.2015. Cloning, expression characteristics and subcellular-location of heat shock transcription factor ZmHsf06 in Zea mays[J]. Journal of Agricultural Biotechnology, 23(1): 41-51.)
[3] 赵立娜, 刘子会, 段硕楠, 等. 2018. 小麦热激转录因子基因TaHsfB2d的克隆和特性及其对耐热性调控[J]. 作物学报, 44(1): 53-62.
(Zhao L N, Liu Z H, Duan S N, et al.2018. Cloning and characterization of heat shock transcription factor gene TaHsfB2d and its regulating role in thermotolerance[J]. ActaAgronomica Sinica, 44(1): 53-62.)
[4] 张玉杰, 张园园, 张华宁, 等. 2018. 小麦热激转录因子基因TaHsfA2e特性及耐热性功能初探[J]. 作物学报, 44(12): 1818-1828.
(Zhang Y J, Zhang Y Y, Zhang H N, et al.2018. Characterization and regulatory roles in thermotolerance of wheat heat shock transcription factor gene TaHsfA2e[J]. Acta Agronomica Sinica, 44(12): 1818-1828.)
[5] 张园园, 赵慧, 张玉杰, 等. 2019.小麦热激转录因子基因TaHsfA2f生物学特性及耐热性调控作用[J]. 农业生物技术学报, 27(05): 69-79.
(Zhang Y Y, Zhao H, Zhang Y J, et al.2019. Biological characteristics and thermotolerance-regulating roles of wheat (Triticum aestivum) heat shock transcription factor gene TaHsfA2f[J]. Journal of Agricultural Biotechnology, 27(5): 825-835.)
[6] Al-Whaibi M H.2011. Plant heat-shock proteins: A mini review[J]. Journal of King Saud University Science, 23(2): 139-150.
[7] Agarwal P, Khurana P.2019. Functional characterization of HSFs from wheat in response to heat and other abiotic stress conditions[J]. Functional & Integrative Genomics, 19(3): 497-513.
[8] Asseng S, Ewert F, Martre, P, et al.2014. Rising temperatures reduce global wheat? production[J]. Nature Climate Change, 5(2): 143-147.
[9] Banti V, Mafessoni F, Loreti E, et al.2010. The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis[J]. Plant Physiology, 152(3): 1471-1483.
[10] Chan-Schaminet K Y, Baniwal S K, Bublak D, et al.2009. Specific interaction between tomato HsfA1 and HsfA2 creates hetero-oligomeric superactivator complexes for synergistic activation of heat stress gene expression[J]. Journal of Biological Chemistry, 284(31): 20848-20857.
[11] Charng Y Y, Liu H C, Liu N Y, et al.2007. A heat-Inducible transcription factor, HsfA2, is required for extension of acquired thermotolerance in Arabidopsis[J]. Plant Physiology, 143(1): 251-262.
[12] Duan S N, Liu B H, Zhang Y Y, et al.2019. Genome-wide identification and abiotic stress-responsive pattern of heat shock transcription factor family in Triticum aestivum L.[J]. BMC Genomics, 20: 257-276.
[13] Fragkostefanakis S, Mesihovic A, Simm S, et al.2016. HsfA2 controls the activity of developmentally and stress-regulated heat stress protection mechanisms in tomato male reproductive tissues[J]. Plant Physiology, 170(4): 2461-2477.
[14] Fragkostefanakis S, SASCHA RÖTH, Schleiff E, et al.2015. Prospects of engineering thermotolerance in crops through modulation of heat stress transcription factor and heat shock protein networks[J]. Plant, Cell & Environment, 38(9): 1881-1895.
[15] Gaffney T, Friedrich L, Vernooij B, et al.1993. Requirement of salicylic acid for the induction of systemic acquired resistance[J]. Science, 261: 6.
[16] Gong B, Yi J, Wu J, et al.2014. LlHSFA1, a novel heat stress transcription factor in lily (Lilium longiflorum), can interact with LlHSFA2 and enhance the thermotolerance of transgenic Arabidopsis thaliana[J]. Plant Cell Reports, 33(9): 1519-1533.
[17] Guo M, Liu J H, Ma X, et al.2016. The plant heat stress transcription factors (HSFs): Structure, regulation, and function in response to abiotic stresses[J]. Frontiers in Plant Science, 7: 114-126.
[18] Hasanuzzaman M, Nahar K, Alam M, et al.2013. Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants[J]. International Journal of Molecular Sciences, 14(5): 9643-9684.
[19] Heerklotz D, Doring P, Bonzelius F, et al.2001. The balance of nuclear import and export determines the intracellular distribution and function of tomato heat stress transcription factor HsfA2[J]. Molecular and Cellular Biology, 21(5): 1759-1768.
[20] Huang Y C, Niu C Y, Yang C R, et al.2016. The heat-stress factor HSFA6b connects ABA signaling and ABA-mediated heat responses[J]. Plant Physiology, 1182-1199.
[21] Hu X J, Chen D D, Mclntyre C L, et al.2017. Heat shock factor C2a serves as a proactive mechanism for heat protection in developing grains in wheat via an ABA‐mediated regulatory pathway[J]. Plant Cell & Environment, 41(1): 79-98.
[22] Jin G H, Gho H J, Jung K H.2013. A systematic view of rice heat shock transcription factor family using phylogenomic analysis[J]. Journal of Plant Physiology, 170(3): 321-329.
[23] Kumar R R, Goswami S, Singh K, et al.2018. Characterization of novel heat-responsive transcription factor (TaHSFA6e) gene involved in regulation of heat shock proteins (HSPs)-a key member of heat stress-tolerance network of wheat[J]. Journal of Biotechnology, 279: 1-12.
[24] Larkindale J, Hall J D, Knight M R, et al.2005. Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermos tolerance[J]. Plant Physiology, 138: 882-897.
[25] Li G L, Zhang H N, Shao H, et al.2019. ZmHsf05, a new heat shock transcription factor from Zea mays L. improves thermotolerance in Arabidopsis thaliana and rescues thermotolerance defects of the athsfa2 mutant[J]. Plant Science, 283: 375-384.
[26] Li Z, Zhang L, Wang A, et al.2013. Ectopic overexpression of SlHsfA3, a heat stress transcription factor from tomato, confers increased thermotolerance and salt hypersensitivity in germination in transgenic Arabidopsis[J]. PLOS ONE, 8(1): e54880.
[27] Lin K F, Tsai M Y, Lu C A, et al.2018. The roles of Arabidopsis HSFA2, HSFA4a, and HSFA7a in the heat shock response and cytosolic protein response[J]. Botanical Studies. 59: 15-23.
[28] Lin Y X, Jiang H Y, Chu Z X, et al.2011. Genome-wide identification, classification and analysis of heat shock transcription factor family in maize[J]. BMC Genomics, 12(1): 76-89.
[29] Liu A L, Zou J, Zhang X W, et al.2010. Expression profiles of class A rice heat shock transcription factor genes under abiotic stresses[J]. Journal of Plant Biology, 53(2):142-149.
[30] Liu H C, Charng Y Y.2013. Common and distinct functions of Arabidopsis class A1 and A2 heat shock factors in diverse abiotic stress responses and development[J]. Plant Physiology, 163(1): 276-290.
[31] Liu H C, Liao H T, Charng Y Y.2011. The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis[J]. Plant Cell & Environment, 34(5): 738-751.
[32] Mishra S K.2002. In the complex family of heat stress transcription factors, HsfA1 has a unique role as master regulator of thermotolerance in tomato[J]. Genes & Development, 16(12): 1555-1567.
[33] Nishizawa A, Yabuta Y, Yoshida E, et al.2006.Arabidopsis heat shock transcription factor A2 as a key regulator in response to several types of environmental stress[J]. Plant Journal, 48(4): 535-547.
[34] Nishizawa-Yokoi A, Nosaka R, Hayashi H, et al.2011.HsfA1d and HsfA1e involved in the transcriptional regulation of HsfA2 function as key regulators for the Hsf signaling network in response to environmental stress[J]. Plant & Cell Physiology, 52(5): 933-945.
[35] Nover N, Bharti K, Döring P, et al.2001. Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?[J]. Cell Stress & Chaperones, 6(3): 177-189.
[36] Ogawa D, Yamaguchi K, Nishiuchi T.2007. High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased themotolerance but also salt/osmotic stress tolerance and enhanced callus growth[J]. Journal of Experimental Botany, 58(12): 3373-3383.
[37] Ohama N, Sato H, Shinozaki K, et al.2016.Transcriptional regulatory network of plant heat stress response[J]. Trends in Plant Science, 22(1): 53-65.
[38] Qian J, Chen J, Liu Y F, et al.2014. Overexpression of Arabidopsis HsfA1a enhances diverse stress tolerance by promoting stress-induced Hsp expression[J]. Genetics and Molecular Research: GMR, 13(1): 1233-1243.
[39] Sahana H, Praveen P, Vikas S, et al.2016. Musashi-mediated expression of JMJD3, a H3K27me3 demethylase, is involved in foamy macrophage generation during mycobacterial infection[J]. PLOS Pathogens, 12(8): e1005814.
[40] Scharf K D, Berberich T, Ebersberger I, et al.2012. The plant heat stress transcription factor (Hsf) family: Structure, function and evolution[J]. Biochim Biophys Acta, 1819(2): 104-119.
[41] Scharf K D, Heider H, Hohfeld I, et al.1998. The tomato Hsf system: HsfA2 needs interaction with HsfA1 for efficient nuclear import and may be localized in cytoplasmic heat stress granules[J]. Molecular and Cellular Biology, 18(4): 2240-2251.
[42] Schramm F, Ganguli A, Kiehlmann E, et al.2006. The heat stress transcription factor HsfA2 serves as a regulatory amplifier of a subset of genes in the heat stress response in Arabidopsis[J]. Plant Molecular Biology, 60: 759-772.
[43] Snyman M, Cronje M J.Modulation of heat shock factors accompanies salicylic acid-mediated potentiation of Hsp70 in tomato seedlings[J]. Journal of Experimental Botany, 59(8): 2125-32.
[44] Sotirios F, Stefan S, Asmaa E S, et al.2018. The repressor and co-activator HsfB1 regulates the major heat stress transcription factors in tomato[J]. Plant, Cell & Environment, 874-890.
[45] Liao W Y, Lin L F, Jheng J L, et al.2016. Identification of heat shock transcription factor genes involved in thermotolerance of octoploid cultivated strawberry[J]. International Journal of Molecular Sciences, 17(12): 2130-2150.
[46] Wang X, Zhuang L, Shi Y, et al.2017. Up-regulation of HSFA2c and HSPs by ABA contributing to improved heat tolerance in tall fescue and Arabidopsis[J]. International Journal of Molecular Sciences, 18(9): 1981-1993.
[47] Xin H B, Zhang H, Chen L, et al.2010. Cloning and characterization of HsfA2 from lily (Lilium longiflorum)[J]. Plant Cell Reports, 29(8): 875-885.
[48] Xue G P, Drenth J, Mcintyre C L.2015. TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets[J]. Journal of Experimental Botany, 66(3): 1025-1039.
[49] Xue G P, Sadat S, Drenth J, et al.2014. The heat shock factor family from Triticum aestivum in response to heat and other major abiotic stresses and their role in regulation of heat shock protein genes[J]. Journal of Experimental Botany, 65(2): 539-557.
[50] Yabuta, Yukinori.2016. Functions of heat shock transcription factors involved in response to photooxidative stresses in\r, Arabidopsis[J]. Bioscience, Biotechnology and Biochemistry, 80(7): 1254-1263.
[51] Yu H D, Yang X F, Chen S T, et al.2012. Downregulation of chloroplast RPS1 negatively modulates nuclear heat-responsive expression of HsfA2 and Its target genes in Arabidopsis[J]. PLoS Genetics, 8(5): e1002669.
[52] Zhang S, Xu Z S, Li P, et al.2013. Overexpression of TaHSF3in transgenic Arabidopsis enhances tolerance to extreme temperatures[J]. Plant Molecular Biology Reporter, 31(3): 688-697.
[53] Zhu X Y, Huang C Q, Zhang L, et al.2017. Systematic analysis of Hsf family Genes in the brassica napus genome reveals novel responses to heat, drought and high CO2 stresses[J]. Frontiers in Plant Science, 8: 1174-1188. |
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