大肠杆菌与金黄色葡萄球菌感染对BEECs细胞凋亡相关因子表达的影响

doi:10.3969/j.issn.1674-7968.2020.06.006

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Abstract Endometritis of cows (Bos taurus) has a significant impact on the fertility of dairy cows and restricts the development of dairy industry. Postpartum bacterial infection is the leading cause of endometritis. The main pathogenic bacteria isolated clinically are Escherichia coli and Staphylococcus aureus. In this study, bovine endometrial epithelial cells (BEECs) were cultured in vitro by two-step enzymatic digestion, and were infected with E.coli and S.aureus at different concentrations with corresponding multiple of infection (MOI). The TUNEL kit was used to detect apoptosis induced by the infection, and the mRNA levels of apoptosis-related genes Bax, Caspase-3 and Bcl-2 were detected by qRT-PCR after 12 h of the infection. The immunocytochemical identification showed that marker proteins (vimentin and keratin 18) of endometrial epithelial cells were positively stained. In E.coli and S.aureus infection groups, the apoptosis rate was the highest at 10⁷ CFU/mL; E.coli and S.aureus infection induced increased expression of Bax and Caspase-3 (highest at 10⁶ and 10⁷ CFU/mL, respectively), and Bcl-2 expression decreased (lowest at 10⁷ CFU/mL). This study indicated that E. coli and S. aureus could cause endometrial epithelial cell apoptosis, which might provide a reference for related mechanism researches on cow endometritis.

Key words： Escherichia coli Staphylococcus aureus Cell apoptosis Bovine endometrial epithelial cells (BEECs)

Received: 16 October 2019

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S823.8+9

Corresponding Authors: *, sjyu@163.com

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	Articles by authors
	ZHU Chen-Fei
	PAN Yang-Yang
	HE Hong-Hong
	ZHAO Ling
	WANG Wei
	WANG Yan-Xiong
	CUI Yan
	YU Si-Jiu

Cite this article:

ZHU Chen-Fei,PAN Yang-Yang,HE Hong-Hong, et al. Effects of Escherichia coli and Staphylococcus aureus Infection on the Expression of Apoptosis-related Factors in BEECs[J]. 农业生物技术学报, 2020, 28(6): 1004-1011.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2020.06.006 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I6/1004

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