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Polymorphism of miR-486 and Its Expression Pattern in Bashby Sheep (Ovis aries) |
ZHANG Wei1,2, WANG Shi-Yin1,*, SHI Guo-Qing2,*, DENG Shuang-Yi1, LIU Xiao-Na1, YANG Li-Wei1 |
1 Xinjiang Agricultural Professional Technological College, Changji 831100, China; 2 Institute of Animal Husbandry and Veterinary Medicine, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China |
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Abstract miR-486 is expressed in animal muscle at a high level and plays a key role in growth and development of myoblast in different tissues. To illuminate the polymorphism and expression pattern of miR-486 in Bashby sheep (Ovis aries), the stem-loop qRT-PCR was used to detect the expression levels in different tissues, and in skeletal muscle tissues of 8 fetal and postnatal periods of Bashby sheep. Then the mutation of miR-486 sequence in Bashby sheep group and its effect on cutting process of precursor miR-486 (pre-miR-486) in skeletal muscle cell were also researched. The result showed that the miR-486 was expressed at a high level in myocardium and skeletal muscle of the 100 d of fetal period of Bashby sheep, moderately expressed in stomach and intestines, and lowly expressed in liver, spleen, lung, skin and brain tissues; The expression levels of miR-486 in skeletal muscle of the 40~100 d of fetal periods were increased gradually, to a peak level on the 100 d of fetal periods then decreased gradually, and to a minimum level on the 120 d of fetal periods then increased continually until the 90 d of postnatal period; A G>C mutation was detected in pre-miR-486 sequence, which located at the first base of upstream of miR-486 mature sequence, the GG, GC and CC genotype could be detected in Bashby sheep group, but 91% individual of them were GC genotype, the further investigation indicated that the CC genotype could significantly up regulate the level of miR-486 in skeletal muscle cell (P<0.05). The present research could provide basic data for further uncovering the regulation mechanism of miR-486 in development of Bashby sheep skeletal muscle and relationship between its expression and excellent meat quality traits of Bashby sheep.
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Received: 30 April 2019
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Corresponding Authors:
*wangshiyinxjnzy@163.com; nkkxyxms@163.com
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