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| Analysis of the Fertility Sensitivity to Photoperiods and Temperatures of Duration of Cultivation of CMS-WA in Rice (Oryza sativa) |
| LIN Qiang1,2, LUO Xi1,2, XIE Hong-Guang1,2, HE Wei1,2, WANG Ying-Heng1,2, CAI Qiu-Hua1,2, ZHANG Jian-Fu1,2,* |
1 Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350018, China;
2 State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Academy of Agricultural Sciences /Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs /Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology, China/South China Bases of National Key Laboratory of Hybrid Rice for China/National Rice Engineering Laboratory of China, Fuzhou 350003, China; |
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Abstract The various degrees sterility fluctuation of CMS (cytoplasmic male sterility) lines directly affects the seeds purity of hybrid rice (Oryza sativa). For analyzing the fertility sensitivity by photoperiods and temperatures of duration of cultivation to CMS-WA (cytoplamic male sterility of wild abortion), 4 temperatures of duration of cultivation and 4 photoperiods were selected to study male fertility changes of 3 CMS-WA lines ('Zhenshan 97A', 'Longtepu A' and 'Jinfu 1A') which had different numbers of minor effect restoring gene. The dark pollen rate by I2-IK (mixed solution of I2 and IK) dyed and rate of florets with fertile anthers were analyzed by linear regression model. For constructing predictive analysis models, and exploring the genetic basis of dark pollen rate by I2-IK dyed and rate of florets with fertile anthers caused by the interaction between temperatures of duration of cultivation and photoperiods and restoring gene with minor effect. The results showed that dark pollen rate by I2-IK dyed and rate of florets with fertile anthers of male sterile lines were importantly affected by the interaction between photoperiods and temperatures of duration of cultivation. Generally, with temperature rising, dark pollen rate by I2-IK dyed and rate of florets with fertile anthers of CMS lines were increasing. With photoperiod increasing, significant impact was observed in 'Longtepu A', but not in 'Jingfu 1A' and 'Zhenshan 97A'. So, 'Longtepu A' should be classified as photo-thermo-sensitive type, it meant that 'Longtepu A' had mutual promotion effect by photoperiods and temperatures of duration of cultivation, and early season propagation and early season production were priority adopted to ensure the purity of seeds. Breeding and purification of photo-thermo-sensitive type CMS lines, should be conducted at short photoperiods and high temperature of duration of cultivation. Thereby the unqualified individual plant or lines could be eliminated as soon as possible. 'Zhenshan 97A' and 'Jingfu 1A' belonged to thermo-sensitive type, and had no obvious photoperiod effect. Low temperature of duration of cultivation was large contribution to propagation and production seed, and high temperature was beneficial to breeding and purification of thermo-sensitive type CMS lines. The result could provide reference basis for breeding, purification, propagation and reproduction of male sterile lines.
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Received: 27 May 2019
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Corresponding Authors:
*jianfzhang@163.com
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