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| Effect of Ubiquitin-conjugating Enzyme Ubc-9 on Intracellular Survival and Cell Apoptosis of Brucella ovis |
| SHAO Zhi-Ran*, LI Qi-Feng*, YI Ji-Hai, ZHANG Huan, CHEN Chuang-Fu**, WANG Zhen** |
| College of Animal Science and Technology, Shihezi University, Shihezi 832000, China |
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Abstract Only one ubiquitin-like protein SUMO (small ubiquitin-related modifier) coupling enzyme E2 has been found in organisms, that is ubiquitin-conjugating enzyme 9 (Ubc-9). In all known organisms, Ubc-9 is highly conserved. In this study, the transcription level of Ubc-9 in mouse (Mus musculus) macrophages infected with Brucella ovis 16M at different time points were detected by qRT-PCR. Then the mouse macrophage cell line model with Ubc-9 gene silencing was constructed by lentivirus packaging technique. Finally, the effect of Ubc-9 gene silencing Brucella on apoptosis of mouse macrophages was analyzed by flow cytometry, and the intracellular bacterial load was detected.The results showed that the intracellular Ubc-9 transcription level in mouse macrophages infected with Brucella for 8 h was significantly higher than that in the control group (P<0.05). In Ubc-9 gene silenced mouse macrophages constructed by lentivirus packaging system, the transcription of Ubc-9 gene was significantly inhibited, and the apoptosis rate induced by Brucella infection was significantly decreased, and the intracellular bacteria load was higher than that in the control group (P<0.05). This study provides basic data for further exploration of the survival mechanism of Brucella in macrophages.
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Received: 25 June 2019
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Corresponding Authors:
** Corresponding authors, ccf-xb@163.com; wzhen2018@shzu.edu.cn
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| About author:: * The authors who contribute equally |
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