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| Cloning and Expression Analysis of MADS-box Gene HaTrans6 in Sunflower (Helianthus annuus) |
| WEI Xiao-Ying, HE Zhuo-Yuan, LEI Dou, SU Zhou, WU Yu, YANG Jun, ZOU Jian* |
| Key laboratory of Southwest Wildlife Resources Protection (Ministry of Education), China West Normal University, Nanchong 637009, China |
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Abstract Flower development has vital significance for the reproduction of plant offspring, and the genes of MADS-box family play an important role in this process. In order to explore the function of the MADS-box gene transcription factor 6 (Trans6) in the flower development of sunflower (Helianthus annuus), HaTrans6 gene (GenBank No. XM_022141206.1) was cloned from sunflower and was analyzed systematically. The information analysis of gene sequence showed that HaTrans6 had typical MADS-box and K-box conserved domains, and HaTrans6 was a candidate member gene of MADS-box. Phylogenetic tree analysis showed that HaTrans6 gene had the closest homologous relationship with Agamous-like 13 gene (AGL13) and AGL6 gene of Arabidopsis thaliana. The expression pattern analysis indicated that HaTrans6 gene was highly expressed in flower and immature seed. The high and stable expression level was observed from floral organ primordial stage to 5 d after flowering, and reached its top level at floral organ primordial stage. In addition, it was shown that HaTrans6 gene was expressed in bract, crown, petal, stamen, pistil and ovary on 5 d before and the very day of flowering, and the highest expression abundance was found in the ovary, followed by bracts. The above results suggested that Hatrans6 gene might play an important role in regulating floral development and early fruit development of the sunflower. The present study provides basic data for further investigation about the function of Hatrans6 gene.
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Received: 27 March 2019
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Corresponding Authors:
zoujian@cwnu.edu.cn
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