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| Isolation and Screening of High Virulent Bacillus thuringiensis Strains Against Lepidopteran Pests in Some Areas of Liaoning Province |
| NI He-Jia, CHENG Xue, WANG Jing, GAO Ze-Ping, WEI Pan-Pan, TAN Jia-Li, SUN Jing, LI Hai-Tao*, GAO Ji-Guo* |
| College of Life Science, Northeast Agricultural University, Harbin 150030, China |
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Abstract Lepidopteran pests cause huge losses to agricultural production every year, and long-term application of chemical pesticides cause serious damage to the ecological environment. Bacillus thuringiensis(Bt) is widely used as microbial insecticide because of its high insecticidal activity and environmental friendliness. The aim of this study was to isolate Bt strains from the soil of Liaoning province and to characterize them on the basis of the morphology of insecticidal crystal proteins (ICPs), the cry gene, the ICPs profiles and the insecticidal activities against lepidopteran pests. The temperature screening method was applied to isolate the wild Bt strains. The cry genotypes were analyzed by restriction fragment length polymorphism PCR (PCR-RFLP). The ICPs profiles were analyzed by SDS-PAGE, and the isolates were tested for their insecticidal activities against 5 species of Lepidopteran pests. A total of 524 soil samples were collected from different areas of Liaoning province, and 66 wild Bt strains were isolated, with a prevalence rate of 12.6%. The ICPs of the strains exhibited varied shapes such as rhombus, sphere, short biconical and irregular shape. The isolated strains expressed ICPs, including 130, 90 and 60 kD proteins. The insecticidal toxicity test showed that Bt strains DD229, DD214-2, YK524 and YK540 had high activities against the tested Lepidoptera pests. The results showed that Bt was abundant in the soils of Liaoning province. And 4 strains that showed high insecticidal activities against Lepidopteran pests which would have good research and application value.
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Received: 23 November 2018
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Corresponding Authors:
* , lihaitao@neau.edu.cn; gaojiguo1961@hotmail.com
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