Effect of Uniconazole on Photosynthetic Characteristics and Genes Expression of Related Transcription Factors for Coix (Coix lachryma-jobi) Seedlings Under Low Temperature Stress
HUANG Yu-Lan*, YUE Cai-Jun, HAN Yi-Qiang, WANG Jing-Wei, WANG Li-Yan, JIA Gui-Yan, SUN Li-Fang
College of Biological Life, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Abstract Coix (Coix lachryma-jobi) is a crop of food and medicine, and has very high nutritional and medicinal value. But its genetic resources are scarce. Uniconazole (S3307) is plant growth retardant belong to triazole category, which can inhibit the biosynthesis of endogenous gibberellin and has obvious effects on plant growth and stress resistance. In order to obtain and dig the genetic data and function of Coix lachryma-jobi and explore the alleviating effect of S3307 on low temperature stress of coix seedlings, 'Yiliao 5' was used as experimental material, Li-6400XTR photosynthetic apparatus and transcriptome sequencing technology were used to explore the effects of S3307 on photosynthetic characteristics and transcription factor gene expression of coix seedlings under low temperature stress. The results showed that addition of 5 mg/L S3307 could significantly increase the chlorophyll contents of coix leaves under low-temperature. Meanwhile, the net photosynthetic rate, transpiration rate and stomatal conductivity enhanced 138.1%, 59.59% and 50.15%, respectively. Through screening analysis and Swiss-Prot database annotation, 4 differentially expressed genes (DEGs) of WRKY transcription factor, 6 DEGs of bZIP (basic leucine zipper), 9 DEGs of bHLH (basic helix-loop-helix) and 6 DEGs of AP2/ERF (APETALA2/ethylene-responsive factor) were obtained. The qRT-PCR was used to analyze the expression of 10 DEGs, and the changing trend was consistent with the results of transcriptome sequencing. The results of this study will help to reveal the mechanism of S3307 on photosynthetic characteristics and transcription factor expression characteristics of coix under low temperature stress, and provide a reference for further research on transcription factor function in response to low temperature stress.
HUANG Yu-Lan,YUE Cai-Jun,HAN Yi-Qiang, et al. Effect of Uniconazole on Photosynthetic Characteristics and Genes Expression of Related Transcription Factors for Coix (Coix lachryma-jobi) Seedlings Under Low Temperature Stress[J]. 农业生物技术学报, 2019, 27(8): 1360-1368.
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