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| Effect of PirAB Toxin from Xenorhabdus nematophila on the Activities of Related Enzymes in Helicoverpa armigera |
| GUO Xiao-Xiao1, YANG Qing2, LIU Shu-Qin1, WANG Qin-Ying1, NANGONG Zi-Yan1* |
1 College of Plant Protection, Hebei Agricultural University, Baoding 071000, China;
2 College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China |
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Abstract Entomopathogenic nematode symbiotic bacteria and insecticidal toxins have been a research hotspot in the field of pest control. PirAB (photorhabdus insect related toxin AB) toxin is a special binary toxin from symbiotic bacteria of entomopathogenic nematodes with high insecticidal activity, and the insecticidal characteristics of the toxin and its effect on the enzyme activity of host insects were carried out in present study. Firstly, the pirAB gene of Xenorhabdus nematophila HB310 was expressed in prokaryotic expression system, and the biological activity of the recombinant PirAB protein was determinated on the 4th instar larvae of Helicoverpa armigera by intracavity injection method, and the influence of PirAB against related enzymes (acetylcholinesterase, carboxylesterase, polyphenol oxidase, tyrosinase and peroxidase) was further detected. The results showed that recombinant PirAB protein demonstrated a high intravenous injection activity (LD50=2.003 μg/larva). After injection of PirAB toxin with LD50 dose, there was no significant difference in acetylcholinesterase activity between the treatment and the control group; Carboxylesterase activity showed a dramatic change of activation, inhibition and re-activation, and tended to be stable after 36 h treatment; Polyphenol oxidase activity also showed a trend of first activation and then inhibition, and the change of activity tended to be stable after 24 h, and was significantly lower than that of the control group (P<0.05). Tyrosinase activity was basically inhibited after toxin treatment, and there was a significant change between 12 and 36 h which decreased sharply and increased again, and the tyrosinase activity was the same as that of the control; The overall change trend of peroxidase activity was not obvious and continued to be lower than that of the control. This study provides basic information for revealing the immune mechanism of host insects against PirAB toxin.
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Received: 28 November 2018
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Corresponding Authors:
ngzyheb@163.com
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