|
|
Genetic Diversity and Origin of Guizhou Pig (Sus scrofa domesticus) Breeds Analyzed by mtDNA D-loop Region |
ZHAO Zhong-Hai1,2,3*, ZHOU Di1,2,5*, LI Hui1,2**, YI Heng-Jie1,2,4, LONG Qing-Meng5, SHI Xin-Cai1,2, BU Xiao-Yan1,2, Chen Lin1,2, YANG Hua-Ting1,2, LI Xing-Cai1,2 |
1 College of Animal Science/ Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China; 2 Key Laboratory of Animal Genetics, Breeding and Reproduction in Guizhou, Guiyang 550025, China; 3 Animal Husbandry Office of Zunyi City, Zunyi 563400, China; 4 Zhengan County Livestock Industry Development Office, Zunyi 563400, China; 5 Testing Center for Livestock and Poultry Germplasm, Guizhou Agricultural and Rural Affairs Office, Guiyang 550018, China |
|
|
Abstract Mitochondrial DNA (mtDNA), as an extranuclear genetic material, carries important genetic information, while its D-loop region sequence reflects the genetic diversity of the species.To assess the genetic diversity of Guizhou pig (Sus scrofa domesticus) species,10 local pig breeds in Guizhou Province and 3 foreign varietes were chosen as the researching object in this study, they were Gaopo pig, Guanling pig, Congjiang xiang pig, Qiannan black pig, Qianbei black pig, Kele pig, Jiangkou Luobo pig, Zongdi hua pig, Baixi pig, Nuogu pig and Meishan pig, Rongchang pig, Yorkshire.The full-length sequence of the D-loop region of mitochondrial DNA was amplified and analyzed by PCR amplification combined with direct sequencing.The research results showed that the full sequence of the D-loop region of the mtDNA of the experimental group was 1 117~1 148 bp, and there were 12 sequences of length. Except for the difference in the base composition of the Zongdi hua pig, there was no difference in the base content of other varieties, which showed the highest content of T, the lowest content of C, and the base A+T content was significantly higher than C+G. Then, 66 polymorphic loci were found in the 149 splicing sequences of the valid sequencing results, and 16 haplotypes were defined, with a haplotype diversity of 0.421 5. The genetic relationship between the Gaopo pig population and other pig populations was randomly selected, and it was found that there was less genetic communication among the populations, and the gene exchange value (Nm) was less than 1. The genetic differentiation index between Gaopo pig and Zongdi hua pig was the highest, and it was 0.412 78, and the genetic differentiation index of Gaopo pig and Qiannan black pig and Guanling pig were lower, indicating that the genetic relationship among the three pigs was relatively close; The genetic diversity analysis showed that the Gaopo pig were closely related to the Qianbei black pig, while the Zongdi hua pig was far related to other 12 varieties. Based on the results of this experiment, it could be inferred that the genetic diversity of 10 local pig breeds in Guizhou was threatened to a certain extent, and it was imperative to properly carry out the conservation of local pig breeds. This study provides theoretical support for further understanding of the origin differentiation and genetic diversity of Guizhou pig breeds, and provides a reference for local pig breed protection.
|
Received: 11 January 2019
|
|
Corresponding Authors:
ellenlihui@sina.cn
|
|
|
|
[1] 边琳鹤, 李勤慎, 冯志云, 等. 2014. 基于mtDNA D-loop进行四种鲤科鱼类种间鉴定研究[J]. 中国农学通报, 30(26): 33-39. (Bian L H, Li Q S, Feng Z Y, et al.2014. Species identification by mtDNA D-loop among four Cyprinid fishes[J]. Chinese Agricultural Science Bulletin, 30(26): 33-39.) [2] 陈祥, 丁玫, 孙娟, 等. 2015. 贵州3个地方猪种的mtDNA D-loop序列遗传多样性分析[J]. 畜牧与兽医, 47(5): 55-59. (Chen X, Ding M, Sun J, et al.2015. Genetic diversity analysis of 3 native pig breeds in Guizhou by mtDNA D-loop sequences[J]. Animal Husbandry and Veterinary Medicine, 47(5): 55-59.) [3] 丁玫, 陈祥, 许厚强, 等. 2014a. 贵州三个地方猪种的系统发育分析[J]. 生物技术通报, 4: 96-101. (Ding M, Chen X, Xu H Q, et al.2014, Phylogenetic analysis of three domestic pig breeds in Guizhou Province[J]. Biotechnology Bulletin, 4: 96-101.) [4] 丁玫, 韦雄, 密国辉, 等. 2014b. 白洗猪线粒体DNA控制区分子遗传变异分析[J]. 中国畜牧杂志, 50(05): 1-5. (Ding M, Wei X, Mi G H, et al.2014. Genetic variation analysis of mitochondrial DNA control region of Baixi pigs[J]. Chinese Journal of Animal Science, 50(05): 1-5.) [5] 郭健康. 2016. 大眼鳜3个野生群体的形态特征及遗传多样性研究[D]. 硕士学位论文, 贵州大学,导师: 安苗, pp. 6.(Guo J K. 2016. Morphologic characters and genentic diversity among three wild populations of Siniperca kneri[D]. Thesis for M.S., Guizhou University, Suppervisor: An M., pp. 6.) [6] 韩果方, 罗治华. 2007. 贵州优良地方猪种简介及保护建议[J]. 贵州畜牧兽医, 31(5): 28-19. (Han G F, Luo Z H.2007. Brief introduction and protection proposal of Guizhou fine local pig[J]. Guizhou Animal Science and Veterinary Medicine, 31(5): 28-19.) [7] 郝荣超, 王国华, 梁菲菲. 2017. 中国南部和西北地区山羊mtDNA D-loop区遗传多样性及群体分化[J]. 农业生物技术学报, 25(2): 258-266. (Hao R C, Wang G H, Liang F F.2017. The genetic diversity of mtDNA D-loop and the differentiation of goats (Capra hircus) in South and northwest of China[J]. Journal of Agricultural Biotechnology, 25(2): 258-266.) [8] 李秀丽. 2015. 马身猪和8个参照种群mtDNA D-loop序列多态性研究[J]. 畜牧与兽医, 47(2): 51-54. (Li X L.2015. Study on polymorphism of mtDNA D-loop sequence in Mashen pig and 8 reference populations[J]. Animal Husbandry and Veterinary Medicine, 47(2): 51-54.) [9] 陆超, 郝林琳, 程云云, 等. 2016. 东北民猪线粒体DNA D-loop遗传多样性与母系起源进行研究[J]. 中国兽医学报, 36(4): 675-678. (Lu C, Hao L L, Cheng Y Y, et al.2016. Genetic diversity of mtDNA D-loop and maternal origin of Min breeds[J]. Chinese Journal of Veterinary Science, 36(4): 675-678.) [10] 綦文晶, 何剑雄, 郭晓萍, 等. 2016. 广西3个猪种线粒体DNA D-loop序列遗传多样性及系统进化研究[J]. 基因组学与应用生物学, 35(9): 2367-2374. (Qi W J, He J X, Guo X P, et al.2016. The genetic diversity and phylogenetic relationships among three Guangxi pig breeds based on sequences of mtDNA D-loop[J]. Genomics and Applied Biology, 35(9): 2367-2374.) [11] 权金强, 苟潇, 赵生国. 2015. 云南地方猪种mtDNA D-loop区遗传多样性研究[J]. 四川农业大学学报, 33(4): 422-428. (Quan J Q, Gou X, Zhao S G, et al.2015. The gentic diversity of mitochondrial DNA D-loop in Yunnan native pigs[J]. Journal of Sichuan Agricultural University, 40(6): 792-799.) [12] 王继英, 郭建凤, 孙守礼, 等. 2009. 山东猪种mtDNA D-loop区部分序列遗传多样性及系统进化研究[J]. 畜牧兽医学报, 40(6): 792-799. (Wang J Y, Guo J F, Sun S L, et al.2009. The genetic diversity and phylogenetic relationships among pig breeds of Shandong province based on partial sequence of mtDNA D-loop region[J]. Acta Veterinariaet Zootechnica Sinica, 40(6): 792-799.) [13] 燕志宏, 田松军, 申红艳, 等. 2010. 宗地花猪1世代的生长发育研究[J]. 安徽农业科学, 38(24): 13107-13108. (Yan Z H, Tian S J, Shen H Y, et al.2010. Research of growth and development for Zongdi hua pig 1 generation[J]. Journal of Anhui Agricultural Sciences, 38(24): 13107-13108.) [14] 张冰, 廖海洪, 农素群, 等. 2015. 广西5个猪种和国外3个猪种mtDNA D-loop序列遗传多样性及进化分析[J]. 中国畜牧兽医, 42(07): 1807-1815. (Zhang B, Liao H H, Nong S Q, et al., 2015. The genetic diversity phylogenetic relationships of five Guangxi pig breeds and three exotic pig breeds by analysis of mtDNA D-loop sequences[J]. China Animal Husbandry and Veterinary Medicine, 42(07): 1807-1815.) [15] Ghivizzani S C, Mackay S L, Madsen C S, et a1.1993. Transcribed heteroplasmic repeated sequences in the porcine mitochondrial DNA D-loop region[J]. Journal of Molecular Evolution, 7(1): 36-37. [16] Kantanen J, Edwards C J, Bradley D G, et al.2009. Maternal and paternal genealogy of Eurasian taurine cattle (Bos taurus)[J]. Heredity, 103(5): 404-415. [17] Kim K I, Lee J H, Li K, et al.2002. Phylogentic relationships of Asian and European pig breeds determined by mitochondrial D-loop sequence polymorphism[J]. Animal Genetics, 33(1): 19-25. [18] Kristina G, Vladimir M, Polona M, et al.2008. Genetic diversity of autochthonous pig breeds analyzed by microsatellite markers and mitochondrial DNA D-loop sequence polymorphism[J]. Animal Biotechnology, 2018: 1-10. [19] Osei-Amponsah R, Skinner B M, Adjei D O, et al.2017. Origin and phylogenetic status of the local Ashanti Dwarf pig (ADP) of Ghana based on genetic analysis[J]. BMC Genomics, 18(1): 193. [20] Pfeiffer I, Voelkel I, Brenig B.2005. Phylogenetics of the European Dahomey miniature cattle based on mitochondrial D-loop region DNA sequence[J]. Animal Genetics, 36(2): 179-181. [21] Randi E, Lucchini V.1998. Organization and evolution of the mitochondrial DNA control region in the avian genus alectoris[J]. Journal of Molecular Evolution, 47(4): 449-462. [22] Yang J, Wang J, Kijas J, et al.2003. Gentic diversity present within the near-completemt DNA genome of 17 breeds of indigenous Chinese pigs[J]. Journal of Heredity, 94(5): 381-385. [23] Zouros E, Ball AO, Saavedra C, et al.1994. Mitochondrial DNA inheritance[J]. Nature, 368: 818 |
[1] |
ZHU Xiao-Feng, XU Hou-Qiang, CHEN Wei, CHEN Ying-Lian, NI Kai, WU Xiao-Min, NI Meng-Meng, LU Xian-Jun. Cloning, Expression and Bioinformatics Analysis of IGF-1 and IGF-2 Genes in Congjiangxiang Pigs (Sus scrofa)[J]. 农业生物技术学报, 2019, 27(8): 1382-1391. |
|
|
|
|