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| Association Between FTO Gene Polymorphism and Serum Lipid Metabolism Indexes in Qianbei Ma Goat (Capra hircus) |
| WENG Ji-Mei, LONG An-Ju, AI Jin-Xin, LUO Zheng, CAI Hui-Fen, LUO Wei-Xing* |
| Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education/College of Animal Science, Guizhou University, Guiyang 550025, China |
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Abstract Fat mass and obesity associated gene (FTO) is an obesity susceptibility gene discovered by genome-wide scanning technology.FTO could regulate fatty acid transport and fat metabolism-related genes by transcription factors and transporters, participating in the development, maintenance and deposition of adipose tissue.Qianbei Ma goat is one of the three local varieties in Guizhou province.It is delicious and has good meat production.In present study, Qianbei Ma sheep (Capra hircus) was taken as the investigated subject, and the association between FTO gene exon polymorphism and serum physiological and biochemical indexes related to lipid metabolism was studied.SNPs were firstly screened by hybrid DNA pool combined with PCR direct sequencing technology.The genotypes of the mutation sites were directly sequenced by single sample.The different genotypes of FTO gene mutations and serum leptin, hormone sensitive lipase (HSL), adiponectin, total cholesterol, triglyceride, high density lipoprotein, low density lipoprotein, alanine aminotransferase (ALT), aspartate aminotransferase (AST) were determined by SPSS 17.0.A total of 9 SNPs were found in the tested goat population: g.C10943T, g.G109518T, g.T125300A, g.C125357T, g.G125405T, gT125417C, g.T426229C, g.C426244G, g.G426347A, where g.G109518T and g.G426347A site mutations were located in the second and ninth intron regions, respectively.Chi-square results showed that each mutation site belonged to moderate polymorphism (0.5<PIC<0.2) and did not deviate from the Hardy-Weinberg equilibrium state.There were 3 genotypes in each mutation site.Correlation analysis showed that the serum AST and ALT levels in g.T125300A and g.G426347A sites were significantly different among different genotypes (P<0.01); There were significant differences in serum AST and ALT levels between g.G125405T and g.T125417C sites (P<0.05); AST differences were significant at g.T426229C sites (P<0.05), while ALT levels were significantly different (P<0.01).Serum HSL levels were significantly different between genotypes at g.T426229C and g.G426347A (P<0.05).It is concluded that the FTO gene g.T125300A, g.G125405T, g.T125417C, g.T426229C and g.G426347A sites have a certain correlation with the lipid metabolism of Qianbei Ma goat which provide a theoretical reference for screening auxiliary selection markers of fat deposition and meat quality regulation in goats.
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Received: 24 October 2018
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Corresponding Authors:
liuqiuyue@caas.cn；mxchu@263.net
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