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| Transcriptome Sequencing of Leaves and Flowers and Screening and Expression of Differential Genes in Aroma Synthesis in Chimonanthus salicifolius |
| QIAN Zheng, ZHANG Si-Jie, JIN You-Quan, ZHANG Xin-Feng* |
| State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China |
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Abstract Chimonanthus salicifolius belongs to Chimonanthus of Calycanthaceae family, the leaves of Ch.salicifolius are shattered with a very fragrant smell, while its flowers smell rather lighter.In order to investigate the key regulation gene and molecular regulation pathway related aroma components in leaves and flowers of Ch.salicifolius, the transcriptome sequencing, sequencing data assembly, and functional annotation and classification of the sequences were conducted by using the leaves and flowers of Ch. salicifolius as materials in this study, and the aroma synthesis related differentially expressed genes were analyzed by qRT-PCR.By using transcriptome sequencing and bioinformatics analysis, 153 059 Unigenes were obtained by De novo assembly in leaves and flowers.Among them, 56 964, 40 746, 22 173, 47 858, 46 196 and 37 029 unigenes were assigned into NR (Non-Redundant Protein Sequence Database), Swiss-Prot (Manually Annotated and Reviewed Protein Sequence Database), KEGG (Kyoto Encyclopedia of Genes and Genomes), Pfam (Protein Family), KOG (euKaryotic Ortholog Groups) and GO (Gene Ontology), respectively.Though the differentially expressed analysis of 2 materials, 5 517 differentially expressed genes were annotated, of which 2 104 genes up-regulated and 3 413 genes down-regulated.After GO and KEGG enrichment analysis, 314 GO annotations and 33 significantly enrichment metabolic pathways were obtained, including 7 aroma synthesis related significant enrichment of metabolic pathways involved 33 different enzymes, seven different enzyme genes expression in leaves were higher than that in flowers, while the remaining 26 enzyme genes expression were lower than that in flowers.Differentially expressed genes and regulation pathways of leaves and flowers of Ch.salicifolius were screened by transcriptional analysis, which showed that the genes AOC (allene oxide cyclase), TAT (tyrosine aminotransferase), CHLP (geranylgeranyl reductase), DXS(1-deoxy-D-xylulose-5-phosphate synthase), TES ((-)-α-terpineol synthase), LMS ((R)-limonene synthase) and GA3OX (gibberellin 3-beta-dioxygenase) high expression in leaves of Ch.salicifolius, and they may have a greater contribution to the formation of aroma components in leaves, promoting the synthetic biomass of aroma components.This study results will provide the valuable genome data sources in molecular biology of Ch.salicifolius.
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Received: 16 November 2018
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Corresponding Authors:
zhangxf73@163.com
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