Resistance Allelism Study of Chinese Cabbage (Brassica campestris spp. pekinensis) Turnip mosaic virus Disease
MAN Wei-Ping1,2,*, ZHANG Zhi-Gang2,*, LIU Shuan-Tao2, WANG Rong-Hua2, WANG Li-Hua2, XU Nian-Fang3, XU Wen-Ling2, LIU Xian-Xian2, LIU Chen2, LI Qiao-Yun1,2,**, ZHAO Zhi-Zhong2,**
1 College of Life Sciences, Shandong Normal University, Jinan 250014, China; 2 Vegetable and Flower Institute of Shandong Academy of Agricultural Sciences/Shandong Branch of National Center for Vegetables Improvement/Shandong Key Laboratory for Biology of Greenhouse Vegetables/Huang-Huai-Hai Region Scientific Observation and Experimental Station of Vegetables, Ministry of Agriculture and Rural Affairs, Jinan 250100, China; 3 Agricultural and Sideline Raw Materials Research Institute on Light Industry of Shandong, Gaomi 261500, China
Abstract Chinese cabbage (Brassica rapa spp. pekinensis) originated in China, and is an important vegetable in China and even in East Asia. Virus disease is one of the most important diseases for Chinese cabbage production, in which Turnip mosaic virus (TuMV) is the main pathogen. The inheritance pattern for TuMV resistance is very complicated in Chinese cabbage. In order to better understand the similarities and differences of resistance sites between different TuMV resistant materials, the high-generation inbred line TuMV resistant material '73' and National TuMV source resistant material '8407' were used as core parents to study the similarities and differences of TuMV resistant sites in 9 resistant materials ('73', '8407', 'Zao 219', '07-372', C07-14', 'Henan 304', '322', '826' and '07-55'). By crossing and inbreeding, F1 and F2 populations of 'Zao219'×'8407', '07-372'×'8407', '07-14'×'8407', 'Henan304'×'8407', '322'×'8407', '826'×'8407', '07-55'×'8407', '73'×'8407', 'Zao219'×'73', '07-372'×'73', 'Henan304'×'73', '322'×'73', '07-55'×'73', '07-372'×'322', 'Henan304'×'322', '07-372'×'Henan304', 'Zao219'×'322', 'Zao219'×'Henan304' and 'Zao219'×'07-372' were constructed. The C4 strains of TuMV was inoculated on the above F1, F2 and parent materials. The similarities and differences of resistance sites among different combinations were judged according to the resistance performance of F1 and the resistance isolation of F2. The results showed, in combinations '73'×'8407' and '322'×'8407', both F1 were resistant, individual susceptible plants were found in both F2 populations, indicating that the resistance sites in '73' and '322' were linked to that in '8407' but not allelic. In combination '07-55'×'8407', F1 was resistant, no susceptible plants were found in F2, but 3 grade 1 and 6 grade 3 plants, indicated that the resistance site in '07-55' and '8407' were incomplete allelic, should be linked. In combinations '322'×'73' and '07-55'×'73', F1 and all of F2 plants were resistant, no susceptible plants were found in F2, indicating that the resistance sites of '322', '07-55' and '8407' were allelic, and their resistance was controlled by the same gene. In combinations 'Zao219'×'8407', '07-372'×'8407', '07-14'×'8407', and '826'×'8407', all of the F1 and F2 plants were resistant, no susceptible plants were found in F2, indicating that the resistance sites of 'Zao219', '07-372', '07-14', '826' and '8407' were allelic, and their resistance were controlled by the same gene. In combination 'Henan304'×'8407', F1 was susceptible, both resistant and susceptible plants were found in F2, and the segregation ratio of resistant and susceptible plants was 9∶7, indicating that the resistance sites of 'Henan304' and '8407' were not allelic, TuMV resistant genes in 'Henan304' and '8407' were complementary. In combination 'Henan304'×'322', F1 was resistant, both resistant and susceptible plants were found in F2, and the segregation ratio of resistant and susceptible plants was 15∶1, indicated that the resistance sites of 'Henan304' and '322' were not allelic, TuMV resistant genes in 'Henan304' and '322' were inherited independently. This result provides a basis for the next step of targeted selection of disease resistant materials and research on the molecular markers of resistance to TuMV in Chinese cabbage. It also broadens the germplasm resources of resistance of Chinese cabbage for TuMV resistant breeding, and provides theoretical guidance for the selection of parents and offspring of resistance breeding of Chinese cabbage to TuMV.
MAN Wei-Ping,ZHANG Zhi-Gang,LIU Shuan-Tao, et al. Resistance Allelism Study of Chinese Cabbage (Brassica campestris spp. pekinensis) Turnip mosaic virus Disease[J]. 农业生物技术学报, 2019, 27(12): 2120-2129.
[1] 白云, 李欣, 张丛卓, 等. 2011.小麦新抗源CH7103抗条锈基因的遗传及其与已知基因的关系[J]. 麦类作物报, 31(02): 364-369. (Bai Y, Li X, Zhang C Z, et al.2011. Inheritance of resistance to stripe rust genes of wheat new resistance source CH7103 and its relationship with known genes[J]. Journal of Wheat Crops, 31(02): 364-369.) [2] 冯兰香, 徐玲, 刘佳, 等. 1988. 北京地区大白菜芜菁花叶病毒株系的鉴定[J]. 中国蔬菜, (04): 11-13.(Feng L X, Xu L, Liu J, et al. 1988. Identification of Turnip mosaic vi- rus strains in Chinese cabbage in Beijing[J]. China Vegetables, (04): 11-13.) [3] 高会超, 曾强, 张志刚, 等. 2016.与大白菜TuMV抗病基因TuRBCS01紧密连锁的分子标记开发[J].农业生物技术学报, 24(02): 196-205. (Gao H C, Zeng Q, Zhang Z G, et al.2016. Development of molecular markers closely linked to the TuMV disease resistance gene TuRBCS01 of Chinese cabbage[J]. Journal of Agricultural Biotechnology, 24(02): 196-205.) [4] 韩和平. 2003.大白菜抗芜菁花叶病毒病基因的AFLP分子标记的研究[D]. 硕士学位论文,中国农业科学院, 导师: 孙日飞. pp. 19-32. (Han H P.2003. AFLP markers linked to Turnip mosaic virus- resistance gene in Chinese cabbage (Brassica rapa L. ssp. pekinensis)[D]. Thesis for M.S., Chinese Academy of Agricultural Sciences, Supervisor: Sun R F. pp. 19-32.) [5] 李海朝, 智海剑, 白丽, 等. 2006. 大豆对SMV株系SC-11的抗性遗传及抗病基因的等位性研究[J]. 大豆科学, (04): 365-368. (Li H C, Zhi H J, Bai L, et al. 2006.The resistance inheritance of soybean to SMV strain SC-11 and the allelicity of disease resistance genes[J]. Soybean Science, (04): 365-368.) [6] 李巧云, 张志刚, 成文华, 等. 2009. 利用ELISA方法鉴定大白菜TuMV抗性[J]. 科技导报, 27(01): 42-45. (Li Q Y, Zhang Z G, Cheng W H, et al.2009. Determination of TuMV resistance of Chinese cabbage by ELISA method[J]. Science and Technology Review, 27(1): 42-45.) [7] 李巧云, 张志刚, 刘栓桃, 等. 2012. 大白菜芜菁花叶病毒病抗性遗传分析[J]. 华北农学报, 27(4): 135-139. (Li Q Y, Zhang Z G, Liu S T, et al.2012. Inheritance analysis of Turnip mosaic virus resistance in Chinese cabbage[J]. Acta Agriculturea Boreali Sinica, 27(4): 135-139.) [8] 刘文睿. 2009. 甜瓜抗蔓枯病基因分子标记及等位性测验研究[D]. 硕士学位论文,南京农业大学, 导师:陈劲枫. pp. 37-41. (Liu W R.2009. Molecular Markers And Allelic Tests of Muskmelon Resistance to Wheat Blight[D]. Thesis for M.S., Nanjing Agricultural University, Supervisor: Chen J F. pp. 37-41.) [9] 钮心恪. 1984. 大白菜抗霜霉病、病毒病原始材料的筛选及抗性遗传的研究[J]. 中国蔬菜, 18(04): 36-41. (Niu X K.1984.Screening of original material resistant to downy mildew and TuMV and its resistant inheritance research[J]. China Vegetables, 18(4): 28-32.) [10] 潘春清. 2007.大白菜AFLP遗传图谱构建及抗TuMV-C3的QTL分析[D]. 硕士学位论文, 东北农业大学, 导师: 文景芝. pp. 14-38. (Pan C Q.2007. Construction of AFLP genetic map and QTL analysis of TuMV- C3 resistance in Chinese cabbage (Brassiea campestris L. ssp. pekinensis)[D]. Thesis for M. S., Northeast Agricultural University, Supervisor: Wen J Z. pp. 14-38.) [11] 钱伟, 张淑江, 章时蕃, 等. 2012. 大白菜TuMV抗性的主基因+多基因混合遗传分析[J]. 中国蔬菜, (12): 16-21. (Qian W, Zhang S J, Zhang S F, et al.2012. Genetic analysis on mixed main gene plus polygenes for TuMV resistance in Chinese cabbage (Brassica campestris L. ssp. pekinensis)[J]. China Vegetables, (12): 16-21.) [12] 屈淑平, 张彤, 张俊华, 等. 2009.大白菜抗TuMV-C3的QTL分析[J]. 东北农业大学学报, 40(11): 33-37. (Qu S P, Zhang T, Zhang J H, et al.2009. Analysis of QTL mapping for TuMV- C3 resistance in Chinese cabbage[J]. Journal of Northeast Agricultural University, 40(11): 33-37.) [13] 孙蕾. 2007. 抗豆象基因等位性鉴定及栽培绿豆V2709抗豆象遗传与分子标记[D]. 硕士学位论文, 中国农业科学院, 导师: 程须珍. pp. 19-23. (Sun L.2007. Allelic identification of anti-bean-like genes and cultivation of mung bean V2709 anti-bean genetic and molecular markers[D]. Thesis for M. S., Chinese Academy of Agricultural Sciences, Supervisor: Cheng X Z. pp. 19-23.) [14] 王大刚. 2010. 大豆对大豆花叶病毒抗性遗传、抗性基因精细定位及表达分析[D]. 博士学位论文, 南京农业大学, 导师: 智海剑. pp. 37-46. (Wang D G.2010. Fine mapping and expression analysis of soybean resistance to Soybean mosaic virus inheritance and resistance genes[D]. Thesis for Ph. D., Nanjing Agricultural University, Supervisor: Zhi H J, pp. 37-46.) [15] 阎瑾琦. 2000.大白菜抗芜菁花叶病毒病基因的 RAPD分子标记[D]. 硕士学位论文,中国农业科学院, 导师: 孙日飞. pp. 24-32. (Yan J Q.2000. RAPD markers linked to Turnip mosaic virus-resistance gene in Chinese cabbage (Brassica rapa L. ssp. pekinensis)[D]. Thesis for M.S., Chinese Academy of Agricultural Sciences, Supervisor: Sun R F. pp. 24-32.) [16] 张晓亮. 2012. 大白菜抗芜菁花叶病毒基因分子标记筛选与定位[D]. 硕士学位论文, 青岛农业大学, 导师: 宋希云. pp. 15-16. (Zhang X L.2012. Identification of molecular markers and mapping for Turnip mosaic virus (TuMV) resistancegene in Chinese cabbage (Brassica rapa L. ssp. pekinensis)[D]. Thesis for M.S., Qingdao Agricultural University, Supervisor: Song X Y, pp. 15-16.) [17] 张晓伟, 原玉香, 王晓武, 等. 2009.大白菜DH群体TuMV抗性的QTL定位与分析[J]. 园艺学报, 36(05): 731-736. (Zhang X W, Yuan Y X, Wang X W.et al.2009. QTL mapping for TuMV resistance in Chinese cabbage (Brassica campestris L. ssp. pekinensis)[J]. Acta Horticulturae Sinica, 36(5): 731-736.) [18] 曾强, 张志刚, 赵智中, 等. 2014.大白菜芜菁花叶病毒抗性遗传及分子标记筛选[J]. 华北农学报, 29(02): 75-79. (Zeng Q, Zhang Z G, Zhao Z Z, et al.2014. Genetic and molecular marker screening of Chinese cabbage Turnip mosaic virus resistance[J]. Acta Agriculturae Boreali, 29(02):75-79.) [19] 周强, 李生荣, 杜小英, 等. 2009. 小麦新品种绵麦39成株期抗条锈性的遗传分析[J]. 中国农报, 25(01): 104-107. (Zhou Q, Li S R, Du X Y, et al.2009.Genetic analysis of resistance to stripe rust in a new wheat variety Mianmai 39[J]. China Agricultural News, 25(01): 104-107.) [20] Huang X Q, Hsam S L K, Mohler V, et al.2004. Genetic mapping of three alleles at the Pm3 locus conferring powdery mildew resistance in common wheat (Triticum aestivum L.)[J]. Genome, 47(6): 1130-1136. [21] Li Q Y, Tong H S, Zhang Z G, et al.2011. Inheritance and development of EST-SSR marker associated with Turnip mosaic virus resistance in Chinese cabbage[J]. Canadian Journal of Plant Science, 91(4): 707-715. [22] Li Q Y, Zhang X L, Zeng Q, et al.2015. Identification and mapping of a novel Turnip mosaic virus resistance gene TuRBCS01 in Chinese cabbage (Brassica rapa L.)[J]. Plant Breeding, 134(2): 221-225. [23] Provvidenti R.1980. Evaluation of Chinese cabbage cuhivars from Japan and the People's Republic of China for resistance to turnip mosaic virus and Caulifower mosaic virus[J]. Journal of the American Society for Horticultural Science, 105(04): 571-573. [24] Rusholme R L, Higgins E E, Walsh J A, et al.2007.Genetic control of broad-spectrum resistance to Turnip mosaic virus in Brassica rapa (Chinese cabbage)[J]. Journal of General Virology, 88(11): 3177-3186. [25] Suh S K, Green S K, Park H G.1995. Genetics of resistance to five strains of Turnip mosaic virus in Chinese cabbage[J]. Euphytica, 81(01): 71-77. [26] Yoon J Y, Green S K, Opena R T.1993. Inheritance of resistance to Turnip mosaic virus in Chinese cabbage[J]. Euphytica, 69(1-2): 103-108.
