热纤梭菌嗜热阿魏酸酯酶在拟南芥中的异源表达

doi:10.3969/j.issn.1674-7968.2019.10.004

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Abstract In plant cell wall, the ferulic acid can form covalent cross-linking in lignin-ferulic acid-arabia xylan complexes with hemicellulose and lignin, which is an important molecular basis for the formation of anti-degradation barrier in lignocellulose. Ferulic acid esterase (FAE) can break the ester bond between hemicelluloses as well as hemicellulose and lignin. In this study, the codon of a thermophilic ferulic acid esterase gene from Clostridium thermocellum was optimized and then constructed into the plant binary expression vector containing different signal peptide coding sequence which the thermophilic FAE protein expressed in Arabidopsis thaliana targeting to the cytoplasm, apoplast, endoplasmic reticulum, chloroplast or mitochondria, respectively. The results indicated that the thermophilic FAE was successfully expressed in different transgenic lines, and with the highest value when expressed in the cytoplasm, its enzyme activity at 70 °C was much higher than that at 25 °C (P<0.05). The plant height, fresh weight and 100-seed weight between different transgenic lines and wild type were not significantly different, but an early flowering was observed in the lines with FAE proteins expressed in chloroplasts or mitochondria. This study can provide new ideas and methods for efficient resource utilization of energy plants, pastures and crop straws.

Key words： Thermophilic ferulic acid esterase Arabidopsis thaliana Transgene Subcellular localization expression Enzyme activity

Received: 08 April 2019

ZTFLH:

S188

Corresponding Authors: jxjiang@ujs.edu.cn

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	Articles by authors
	NI Xing-Nan
	WANG Yong-Li
	WU Yan-Fang
	LI Xia
	GAO Lu
	JIANG Jian-Xiong

Cite this article:

NI Xing-Nan,WANG Yong-Li,WU Yan-Fang, et al. Heterologous Expression of Thermophilic Ferulic Acid Esterase from Clostridium thermocellum in Arabidopsis thaliana[J]. 农业生物技术学报, 2019, 27(10): 1742-1750.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2019.10.004 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2019/V27/I10/1742

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