Maternal Genetic Structure Analysis of Experimental SPF Yorkshire (Sus scrofa) and Landrace Population
XIN Chang1, 2, GAO Cai-Xia2, QUAN Jin-Qiang2, MA Lu-Lu2, CHEN Hong-Yan1, 2, *
1 College of Life Science, Northeast Agricultural University, Harbin 150030, China; 2 State Key Laboratory of Veterinary Biotechnology, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
Abstract The genetic quality control research is very necessary for specific pathogen free (SPF) Yorkshire (Sus scrofa) and Landrace pig populations used as the important experimental animals. To analyze the genetic diversity of the mitochondrial DNA (mtDNA) displacement-loop region (D-loop region) and reveal the maternal genetic structure of experimental specific pathogen free (SPF) Yorkshire and Landrace population, genomic DNA of SPF Yorkshire and Landrace pigs were extracted, the mtDNA D-loop region was amplified by PCR, and the sequences were aligned. The local Bama miniature pigs and Rongshui miniature pigs were used as control. A total of 40 variable sites were identified in 107 samples, including 13 haplotypes. Compared with the local breeds, the fewer haplotypes were found in the Yorkshire and Landrace, which contained 3 and 2 specific haplotypes, respectively, and one shared haplotype H4. There was one dominant haplotype in each of the two breeds. Analysis result of the haplotype diversity (Hd) showed that the Rongshui miniature pigs was the highest (0.767) and Landrace was the lowest (0.429). Yorkshire and Bama miniature pigs were 0.566 and 0.503, respectively. In addition, the results of network and phylogenetic tree showed that the genetic relationship between Yorkshire and Landrace populations was closely related to European pig breeds. The study indicated that there was less haplotypes and lower genetic diversity in SPF Yorkshire and Landrace populations. Only one dominant haplotype for each breed was observed. This study will be beneficial for the further research of experimental animal standardization and genetic quality control.
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