不同发育阶段小尾寒羊皮肤毛囊特异性miRNA的鉴定

doi:10.3969/j.issn.1674-7968.2019.01.010

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Abstract Small Tail Han sheep (Ovis aries) is a sheep breed with both excellent meat and fur in China, and famous for its fast growing speed and high lambing rate. microRNA (miRNA) is post-transcriptional regulator factor which is involved in the regulation of development of skin and hair follicles in various animals. In the present study, in order to illustrate this regulation in Small Tail Han sheep, the miRNA expression profiles of the skin and hair follicles during adult (XWHY_1), postnatal 30 d (XWHY_2) and newborn (XWHY_3) were established and analyzed by using high throughput sequencing technology. The results showed that total 1 062 miRNAs, including 160 known miRNAs and 902 new miRNAs, were identified in these 3 developmental periods. By statistical analysis of these miRNAs, it was found that there were 69, 127, and 73 specifically expressed miRNAs in XYHY_1, XYHY_2 and XYHY_3, respectively. Total 666 miRNAs were commonly expressed in the 3 periods. Statistical analysis of differential expression of miRNAs was conducted by Fisher exact test and Chi-square test, and meet the condition of |log₂(Fold_Change, FC)|≥1 and P≤0.05. By comparing XWHY_1, XWHY_2 and XWHY_3, 339 differentially expressed microRNAs were screened, 226 differentially expressed microRNAs were obtained after removing the repetition, and 2 645 target genes were predicted. Differentially expressed miRNAs were enriched by Kyoto encyclopedia of genes and genomes (KEGG) Pathway, results showed that 2 645 target genes were annotated to 181 signaling pathways. Differentially expressed of miRNAs was enriched by gene ontology (GO) and showed to be mainly participated in transcriptional regulation, formation of cell membrane and ion binding. The present study provides basic data to analyze the molecular mechanism of fur/velvet formation at miRNA level, and also provides a new thought to improve the yield and quality of wool/velvet of Small Tail Han sheep by means of manual regulation while maintaining its existing excellent quality.

Key words： miRNA High-throughput sequencing Skin Hair follicle Small Tailed Han sheep

Received: 07 May 2018

ZTFLH:	S826
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Corresponding Authors: *, wuyh_77@126.com

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	HE Yu-Long
	TAO Yu
	LI Yong
	YANG Yi
	ZHOU Xue-Zhang
	WU Yue-Hong

Cite this article:

HE Yu-Long,TAO Yu,LI Yong, et al. Identification of Specific miRNA in Skin Follicles of Small Tail Han Sheep (Ovis aries) at Different Developmental Stages[J]. 农业生物技术学报, 2019, 27(1): 89-96.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2019.01.010 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2019/V27/I1/89

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