基于宏基因组茉莉花植株土壤细菌多样性研究

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (5728 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract The soil microbial diversity has been characterized as an important soil quality indicator. In this study, the soil bacterial community constitution of Jasminum sambac Ait was analyzed by the high throughput sequencing (Illumina Miseq) of the 16S rRNA V3-V4 hypervariable region, to investigate differences of the soil bacterial community structure among different months during the jasmine flower season and the relatedness between community structure of bacteria and soil physic-chemical properties. The results indicated that the soil bacterial diversity of J. sambac was very rich, including 41 phyla, 98 classes, 225 orders, 427 famlies and 799 genera. The 6 phyla with high abundance were Proteobacteria, Acidobacteria, Chloroflexi, Actinobacteria, Bacteroidetes and Firmicutes. The most abundant phylum in each month was Proteobacteria (22.0%~28.4%). The abundance of the phylum Acidobacteria was the lowest (11.7%) in June, suggesting the best soil quality in this month. The Shannon index and Simpson index were the maximum (6.9264) and the minimum (0.002901) in October, respectively, which reflecting the highest bacterial diversity. These results demonstrated that the low temperature was beneficial to increase the soil bacterial diversity. The results of clustering and principal component analysis showed that the bacterial community structure of the soil samples in July, August and September possessed the highest similarity, while those of the soil sample in June and October exhibited obviously difference. Heatmap and redundancy analysis indicated that soil bacterial diversity were significantly influenced by soil properties including the soil pH, organic matter, total and alkali hydrolyzable nitrogen, total and available phosphorus, total and available potassium. Among them, pH, total phosphorus and available potassium were the three most important environmental factors. Moreover, there were positive correlations between the three main bacterial phyla (Firmicutes, Actinobacteria and Chloroflexi) and the six soil environmental factors, including pH, organic matter, total nitrogen, available nitrogen, total phosphorus and total potassium. This work provides useful informations for field management of J. sambac during flower season and soil conservancy ecological balance.

Key words： Jasminum sambac Ait Soil bacteria Diversity Soil physic-chemical properties

Received: 19 January 2018 Published: 06 August 2018

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	CHEN Mei-Chun
	ZHU Yo-Jing
	LIU Bei
	YU Jie-Beng
	LIU Xiao-Hong
	YANG Wen-Jing

Cite this article:

CHEN Mei-Chun,ZHU Yo-Jing,LIU Bei, et al. Diversity Research of the Soil Bacteria of Jasminum sambac Ait Based on Metagenome[J]. , 2018, 26(9): 1480-1493.

URL:

http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2018/V26/I9/1480

陈承利，廖敏，曾路生. 2006. 污染土壤微生物群落结构多样性及功能多性测定方法[J]. 生态学报， 26(10): 3404?3412. (Chen CL, Liao M, Zeng LS. 2006. Methods to measure the microbial community structure and functional diversity in polluted soils [J]. Acta Ecologica Sinica, 26(10): 3404?3412.)
陈月星，温晓霞，孙瑜琳，等. 2015. 地表覆盖对渭北旱作苹果园土壤细菌群落结构及多样性的影响[J]. 微生物学报，55(7): 892-904. (Chen Y X, Wen XX, Sun YL, et al. 2015. Effect of ground mulch managements on soil bacterial community structure and diversity in the non-irrigated apple orchard in Weibei Loess Plateau [J]. Acta Microbiologica Sinica, 55(7): 892-904)
董志新. 2010. 气候条件变化对不同农田土壤微生物多样性的短期与长期影响[D]. (Dong Z X. 2010. Short and Long Term Impacts of Climate Change on Soil Microbial Diversity in Different Croplands [D])
何容，王国兵，汪家社，等. 2009. 武夷山不同海拔植被土壤微生物量的季节动态及主要影响因子[J]. 生态学杂志, 28(3): 394–399. (He R, Wang G B, Wang J S, et al. 2009. Seasonal dynamics and influencing factors of soil microbial biomass in different elevation vegetation in Wuyi Mountain [J]. Chinese Journal of Ecology, 28(3): 394–399)
李晨华, 张彩霞, 唐立松, 等.2014. 长期施肥土壤微生物群落的剖面变化及其与土壤性质的关系[J]. 微生物学报, 54(3): 319–329. (Li CH, Zhang CX, Tang L S, et al. 2014. Effect of long-term fertilizing regime on soil microbial diversity and soil property [J]. 54(3): 319–329)
李巍，刘洋，罗钦, 等. 2017. 武夷山常绿阔叶林土壤微生物多样性的季节动态[J]. 热带亚热带植物学报. 25(2): 115?126.(Li W, Liu Y, Luo Q, et al. 2017. Seasonal Dynamics in Soil Microorganisms Diversity of Evergreen Broadleaved Forest in Wuyi Mountains,Southeastern China [J]. Journal of Tropical and Subtropical Botany, 25(2): 115?126)
李小容，韦金玉，陈云等. 2014.海南岛不同林龄的木麻黄林地土壤微生物的功能多样性[J].植物生态学报，38(6): 608?618. (Li X R, Wei J Y, Chen Y, et al. 2014. Functional diversity of soil microorganisms in Casuarina equisetifolia woodlands of different stand ages in Hainan Island [J]. Chinese Journal of Plant Ecology, 38(6): 608-618)
刘兴, 王世杰, 刘秀明, 等. 2015. 贵州喀斯特地区土壤细菌群落结构特征及变化[J]. 地球与环境, 43(5): 490–497. (Liu X, Wang S J, Liu X M, et al. 2015. Structure and Variation of Soil Bacterial Community in Karst Region of Guizhou Province [J]. Earth and Environment, 43(5): 490–497)
孟祥静，何丽斯，夏冰，等. 2009. 我国茉莉的研究现状及展望[J]. 安徽农业科学, 37(21): 9946?9998.(Meng JX, He LS, Xia B, et al. 2009. Present situation and prospect of research on Jasminum sambac in China [J]. Journal of Anhui Agricultural Science, 37(21): 9946?9998)
汪峰. 2014. 典型农田土壤中微生物群落的长期和短期演变特征及其影响因素[D].(Wang F. 2014. Long - term and short - term evolutionary characteristics of microbial communities in typical farmland soils and their influencing factors [D])
汪旭明，曾冬萍，闵庆文，等. 2015. 福州茉莉花种植园土壤化学计量比及其对碳释放潜力的影响[J]. 中国水土保持科学, 13(1): 118?126. (Wang XM, Zeng DP, Min QW, et tal. 2015. Soil stoichiometric ratio and its effect on carbon release potential of Jasminum sambac plantations in Fuzhou[J]. Science of Soil and Water Conservation, 13(1): 118?126)
文倩, 林启美, 赵小蓉, 等. 2008. 北方农牧交错带林地、耕地和草地土壤微生物群落结构特征的 PLFAs 分析[J]. 土壤学报, 45(2): 321?327. (Wen Q, Lin QM, Zhao XR, et al. 2008. PLFAs Analysis of Soil Microbial Community Structure Characteristics in Forestland, Farmland and Grassland in the Farming-pastoral Interlocked Areas in North China [J]. Acta Pedologica Sinica, 45(2): 321?327)
夏北成, Zhou Jizhong, Tiedje J M. 1998.植被对土壤微生物群落结构的影响[J].应用生态学报， 9(3): 296?300. (Xia B C, Zhou J Z, Tiedje J M. 1998. Effect of vegetation on structure of soil microbial community [J]. Chinese Journal of Applied Ecology, 9(3): 296-300. )
夏围围, 贾仲君. 2014. 高通量测序和DGGE分析土壤微生物群落的技术评价[J]. 微生物学报, 54(12): 1489?1499. Xia WW, Jia ZJ. 2014. Comparative analysis of soil microbial communities by pyrosequencing and DGGE [J]. Acta Microbiologica Sinica, 54(12): 1489?1499.
徐华勤, 肖润林, 邹冬生, 等. 长期施肥对茶树根际土壤微生物群落功能多样性的影响[J]. 生态学报, 2007, 27(8): 3355?3361. (Xu HQ, Xiao RL, Zhou DS, et al. 2007. Effects of long-term fertilization on functional diversity of soil microbial community of the tea plantation [J]. Acta Ecologica Sinica, 2007, 27(8): 3355?3361.
张海涵, 唐明, 陈辉, 等. 2007. 不同生态条件下油松 (Pinus tabulaeformis) 菌根根际土壤微生物群落[J]. 生态学报, 27(12): 5463?5470.(Zhang HH, Tang M, Chen H, et al. 2007. Microbial communities in Pinus tabulaeformis mycorrhizosphere under different ecological conditions [J]. Acta Ecologica Sinica, 27(12): 5463?5470)
张俊华，张翼，李明. 2017. 药用植物宁夏枸杞 (Lycium barbarum L.) 土壤细菌群落演替特征. 植物营养与肥料学报, 23(3): 686–695. (Zhang J H, Zhang Y, Li M. 2017. Evolution characteristics of bacteria in soil of medical plant（Lycium barbarum L.）[J]. Plant Nutrition and Fertilizer Science, 23(3): 686–695)
张伟, 朱艳蕾, 朱晓莹, 等. 2013. 新疆贫营养环境微生物数量及多样性. 应用与环境生物学报, 19(2): 370?374. (Zhang W, Zhu Y L, Zhu XY, et al. 2013. Microbial Community Diversity and Quantity of Xinjiang Oligotrophic Environment [J]. Chinese Journal of Applied & Environmental Biology, 19(02):370-374)
郑雪芳，苏远科，刘波，等. 2010. 不同海拔茶树根系土壤微生物群落多样性分析[J]. 中国生态农业学报, 18(4): 866?871.(Zheng XF, Su YK, Liu B, et al. 2010. Microbial community diversity in tea root zone soils at different elevations [J]. Chinese Journal of Eco-Agriculture, 18(4): 866?871)
庄伟伟，郑李娟，曾献春. 2017. 骆驼瘤胃内降解含氮杂环化合物细菌的多样性[J]. 微生物学报, 57(5): 748?757. (Zhuang W W, Zheng LJ, Zeng X C. 2017. Diversity of camel rumen bacteria degrading nitrogen heterocyclic compounds [J]. Acta Microbiologica Sinica, 57(5): 748?757)
Amann R L, Ludwig W, Schleiffer K H. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation[J]. Microbiol Rew, 59: 143?146.
Arab H G D E, Vlich V, Sikora R A. 2001. The use of phospho-lipids fatty acids(PLFA)in the determination of rhizosphere specific microbial communities of two wheat cultivars. Plant & Soil, 228: 291?297.
Gomez E, Ferreras L, Toresani S. 2006. Soil bacterial functional diversity as influenced by organic amendment application [J]. Bioresource Technology, 97(13): 1484?1489.
Han X M,Wang RQ, Liu J,et al. 2007. Effects of vegetation types on soil microbial community composition and catabolic diversity assessed by polyphasic methods in North China [J]. J Environ Sci, 19: 1228?1234.
Jenkinson DS, Ladd J N. 1981. Microbial biomass in soil: measurement and turnover [J]. Soil Biochemistry, 5: 415?471.
Kimura M, Asakawa S. 2006. Comparison of community structures of microbiota at main habitatsin rice field ecosystems based on phospholipid fatty acid analysis[J]. Biol Fertil Soils, 43: 20?29.
Maguran A E. 1998. Ecological diversity and its measurement[M]. Princeton: Princeton University Press, 141?162.
Nacke H, Thurmer A, Wollherr A, et al. 2011. Pyrosequencing- based assessment of bacterial community structure along different management types in German forest and grassland soils [J]. PLo S One, 6(2): e17000.
Singh D, Shi J L, Adams JM. 2013. Bacterial diversity in the mountains of south-west China: climate dominates over soil parameters [J]. J Microbiol, 51(4): 439–471.
Susanne K, Veronica A M, Husein A. 2006. Microbial community composition and enzyme activities in a sandy loam soil after fumigation with methyl bromide or alternative biocides[J]. Soil Biology & Biochemistry, 38: 1243?1254.
Torsvik V, ?vreas I. 2002. Microbiol diversity and function in soil: From genes to ecosystems[J]. Current Opinion Microbiol, 5: 240?245.
Xia LC, Steele JA, Cram JA, et al. 2011. Extended local similarity analysis (eLSA) of microbial community and other time series data with replicates. BMC Systems Biology, 5(S2): S15.
Zhang Y G, Gong J, Lu H, et al. 2014. An integrated study to analyze soil microbial community structure and metabolic potential in two forest types [J]. PLo S One, 9(4): e93773.