地方黄牛MyoD1基因启动子多态性分析及其相关转录因子SF1验证

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Abstract Abstract The formation of skeletal muscle affects the growth and development of animals. Through screening SNPs of MyoD1 gene promoter in different breeds of cattle (Bos taurus), and studying its effect on growth body size traits and promoter function, to further provide research basis for the molecular genetic markers of growth related traits and MyoD1 gene promoter revealed promoter functional components. 200 Guizhou native cattle (including Guanling cattle, Sinan cattle, Liping cattle, Weining cattle, 50 heads , respectively) as the research material, the experiment amplified MyoD1 promoter and used direct sequencing method to detect SNPs. The experiment found 5 SNPs in four groups, g.-1141T>C、g.-871C>T in Guanling cattle, Sinan cattle, Liping cattle and g.-1778T>C、g.-871C>T、g.-797T>C、g.-761T>C in Weining cattle; Correlation analysis results showed that in Guanling cattle -1141 T>C, g.-871C>T sites had significant influence on abdominal extension girth, body length and chest girth(P＜0.05), respectively; in Liping cattle -1141T>C, g.-871C>T sites had significant influence on cannon circumference(P＜0.05); in Sinan cattle g.-1141T>C site had significant influence on hucklebone width, while g.-871C>T had no significant influence on growth traits; in Weining cattle g.-1778T>C、g.-871C>T、g.-797T>C、g.-761T>C sites had significant influence on body length, hucklebone width and wasitband height(P＜0.05). Using the yeast one-hybrid (Y1H) system, the results found the SF1 transcription factor could be combined with the MyoD1 promoter; using dual luciferase report system, SF1 transcription factor increased the MyoD1 promoter activity. When the SF1 transcription site produced g.-1141T>C mutation, it had significant effect on abdominal girth, cannon circumference and hucklebone width and so on growth traits in three cattle(Guanling cattle, Liping cattle, Sinan cattle). SF1 has important influence for the activation and regulation of the MyoD1 gene.

Key words： MyoD1gene Promoter SF1 transcription factor Yeast single-hybridization experiment

Received: 05 September 2017 Published: 02 May 2018

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	ZHOU Di
	XU Hou-Jiang
	CHEN Wei
	DIAO Jia-Fu
	DUAN Zhi-Jiang

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ZHOU Di,XU Hou-Jiang,CHEN Wei, et al. Polymorphism Analysis of MyoD1 Gene Promoter and Related SF1 Transcription Factors Validation in Local Cattle (Bos taurus)[J]. , 2018, 26(5): 743-755.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2018/V26/I5/743

[1]Collins C A,Olsen I,Zammit P S,Heslop L,Petrie A,Partridge T A,Morgan J E.Stem cell function,self-renewal,and behavioral heterogeneity of cells from the adult muscle satellite cell niche[J].Cell.200,122(2):289－301.
[2]Fan C M,Li L,Rozo M E.Making skeletal muscle from progenitor and stem cells:Development versus regeneration[J].Wiley Interdiscip Review Developmental Biology,2012,1(3):315－327.
[3]Kim T H,Barrera L O,Qu C,Van Calcar S,Trinklein N D,CooPer S J,Luna R M,Glass C K,Rosenfeld M G,Myers R M,RenB.Direet isolation and identification of Promoters in the human genome.GenomeRe,2005,15:830－839.
[4]Bowlin KM, Embree LJ, Garry MG, et al. Kbtbd5 is regulated by MyoD and restricted to the myogenic lineage[J]. Differentiation, 2013, 86(4-5): 184-191.
[5]李永平，梁炳生．MyoD肌形成作用机制研究进展[J]．国际骨科学杂志，2007，28 (1)：37－40．
LI Y P,LIANG B S.Progress of muscle formation mechanism of MyoD. International Journal of Orthopaedics,2007，28 (1)：37－40.(in Chinese)
[6]Melguizo C, Prados J, Sarrat R, et al. Myogenic differentiation induced by actinomycin D in human rhabdomyosarcoma cells: modification in the expression of MyoD and Id[J]. European Journal of Anatomy, 2017.
[7]吴淑疆,晁哲.海南五指山猪Myf5与MyoD 基因多态性分析[J].中国畜牧兽医,2015,42(6):1524-1529.
WU S J,CHAO Z.Polymorphism Analysis of Myf5 and MyoD Genes in Hainan Wuzhishan Pig[J]. China Animal Husbandry and Veterinary Medicine,2015,42(6):1524-1529.(in Chinese)
[8]李万贵,张依裕,王单单. 樱桃谷鸭MyoD1基因外显子4多态与体尺的关联效应研究[J]. 基因组学与应用生物学,2015,(05):950-954.
LI W G,ZHANG Y Y,WANG D D.The Polymorphism of MYOD1 Gene Exon 4 and Its Association with Body Size in Cherry Valley Duck[J].Genomics and Applied Biology, 2015, Vol.34, No.5, 950-954(in Chinese)
[9]G?Blancolizana,C?Garcíafernández,JA?Sánchez?.Searching?SNPs?Variants?in?Myod-1?and?Myod-2?Genes?Linked?to?Body?Weight?in?Gilthead?Seabream,?Sparus?aurata?L[J].?World?Academy?of?Science,?Engineering?and?Technology?International?Journal?of?Animal?and?Veterinary?Sciences?Vol:4,?No:5,?2017.
[10]李飞,许厚强,陈伟,等.关岭牛MyoD1基因启动子报告质粒的构建及活性检验[J].基因组学与应用生物学报,2013,32(4):459-466.
LI F,XU H Q,CHEN W et al.Verification of Activity and Construction of the Report Plasmid of MyoD1 Gene Promoter in Guanling Cattle.Genomics and Applied Biology, 2013, Vol.32, No.4, 459-466.(in Chinese)
[11]张雄, 张勇, 陈祥,等. 猪肌分化因子1基因启动子区多态性及生物信息学研究[J]. 中国畜牧兽医, 2016, 43(5):1308-1315.
ZHANG X,ZHANG Y,CHEN,X,et al.Study on the polymorphisms and bioinformatics promoter region of MyoD1 gene in pig[J].China Animal Husbandry and Veterinary Medicine,2016, 43(5):1308-1315.(in Chinese)
[12]张雯, 许厚强, 陈伟,等. 关岭牛MyoD基因家族对MyoD1启动子活性的影响[J]. 中国农业科学, 2016, 49(6):1196-1206.
ZHANG W,XU H Q,CHEN W,et al.Influence of MyoD1 Promoter Activity by MyoD Family of Guanling Cattle[J].Scientia Agricultura Sinica,2016, 49(6):1196-1206.(in Chinese)
[13]吴俊静, 梅书棋, 彭先文,等. 肉质相关基因TCAP启动子与转录因子MyoD结合的ChIP分析[J]. 湖北农业科学, 2013, 52(22):5612-5614.
WU J J,MEI S Q,PENG X W,et al.Detection of MyoD Transcription Factor Binding with TCAP Gene Promoter by Chromatin Immunoprecipitation[J].Hubei Agricultural Sciences,2013, 52(22):5612-5614.(in Chinese)
[14] Deng B, Zhang F, Chen K, et al. MyoD promotes porcine PPARγ gene expression through an E-box and a MyoD-binding site in the PPARγ promoter region[J]. Cell and tissue research, 2016: 1-11.
[15]赵丹丹,刘聪聪,贾明玉,等.肌肉特异性基因启动子的上游转录调控元件研究进展[J]..中国细胞生物学学报,2012(5):500-505.
ZHAO D D,LIU C C,JIA M Y,et al.Progress of Upstream Transcriptional Control Elements in Promoters of Skeletal Specific Genes[J].
Chinese Journal of Cell Biology ,2012, 34(5): 500–505.(in Chinese)
[16]Borensztein M，Monnier P，Court F．MyoD and H19-Igf2 locus interactions are required for diaphragm formation in the mouse[J].Development,2013,140(6):1231－1239.
[17]王秋岩, 何淑雅, 马云,等. 启动子分析方法的研究进展[J]. 现代生物医学进展, 2015, 15(14):2794-2800.
WANG Q Y, HE S Y,MA Y,et al.Advances in Analytical Methods of Promoter[J].Progress in Modern Biomedicine,2015, 15(14):2794-2800.(in Chinese)
[8] Luo D, De M A, Boutet S, et al. Deltex2 represses MyoD expression and inhibits myogenic differentiation by acting as a negative regulator of Jmjd1c.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2017, 114(15):E3071.
[19]Blum R, Dynlacht B D. The role of MyoD1 and histone modifications in the activation of muscle enhancers[J]. Epigenetics Official Journal of the Dna Methylation Society, 2013, 8(8):778.
[20] Knoll A, Nebola M, Dvorka J, et al. Detection of a DdeⅠPCR-RFLP within intron1 of the porcine (MYF3) locus[J]. Animal Genetics, 1997,28(4):308-322.
[21] 朱励,李学伟. MyoD基因在不同猪种中的PCR-RFLP遗传多态性及其遗传效应研究[J].畜牧兽医学报,2005,35(8):761-766.
ZHU L, L2005,35(8):761-766I X W.The Genetic Diversity and Genetic Effects of MyoD Gene in Different Pig Breeds[J].Chinese Journal of Animal and Veterinary Sciences,2005,35(8):761-766.(in Chinese)
[22]Samarut E, Rochetteegly C. Nuclear retinoic acid receptors: conductors of the retinoic acid symphony during development.[J]. Molecular & Cellular Endocrinology, 2012, 348(2):348-360.
[23]Fong Y W, Cattoglio C, Yamaguchi T, et al. Transcriptional Regulation by Coactivators in Embryonic Stem Cells[J]. Trends in Cell Biology, 2012, 22(6):292-8.
[24]Lala D S, Rice D A, Parker K L. Steroidogenic factor I, a key regulator of Steroidogenic enzyme expression, is the mouse homolog of fushi tarazu-faclor I[J]. Molecular Endocrinology, 1992, 6(8):1249.
[25]Wang L,?Cao C,?Zheng S et al.Transcriptomic?analysis?of?short-fruit?1?(sf1)?reveals?new?insights?intothe?variation?of?fruit-relatedtraits?in?Cucumis?sativus.Sci Rep.?2017 ,Jun 7,7(1):2950
[26]张雯,许厚强,陈伟,等.关岭牛MyoD1基因启动子上转录因子结合位点的筛选[J].畜牧兽医学报,2015,46(7):1259-1267.
ZHANG W,XU H Q,CHEN W,et al.The Screening of Transcription Factor Binding Sites of MyoD1 Promoter in Guanling Cattle[J].
Chinese Journal of Animal and Veterinary Sciences,2015,46(7):1259-1267.(in Chinese)
[27]Xu P, Tan X, Zhang Y, et al. Cloning and expression analysis of myogenin from flounder (Paralichthys olivaceus) and promoter analysis of muscle-specific expression. Comp Biochem Physiol B Biochem Mol Biol, 2007; 147(1): 135-45。
[28]Buckingham M, Relaix F. The role of Pax genes in the develop-ment of tissues and organs: Pax3 and Pax7 regulate muscle progenitor cell functions. Annu Rev Cell Dev Biol 2007; 23: 645-73
[29]Peng XL, So KK, He L, et al. MyoD and FoxO3-mediated hotspot interaction orchestrates super-enhancer activity during myogenic differentiation[J]. Nucleic acids research, 2017.
[30]Davegrdh C, Broholm C, Perfilyev A, et al. Abnormal epigenetic changes during differentiation of human skeletal muscle stem cells from obese subjects[J]. Bmc Medicine, 2017, 15(1):39.