|
|
|
| Cloning of ClHA2 Gene and Its Expression Analysis in Chinese Fir (Cunninghamia lanceolata) |
| , , |
|
|
|
|
Abstract Abstract Respond to low-phosphorus stress, the plasma membrane H+-ATPase plays an important role in plant. In this study, a PM H+-ATPase gene sequence was screened from the transcriptome of Chinese fir . The sequence of PM H+-ATPase were cloned from three Chinese fir clones(X6,3 and 39) respectively. The resulting sequence was 2898bp in length, containing a 2862bp complete open reading frame encoded 953 amino acids residues, named as ClHA2 (Accession No.). This amino acids sequence analysis showed that ClHA2 was highly conserved with PM H+-ATPase of other plants. The predicted relative molecular weight of the protein was about 105.05 kDa, the theoretical isoelectric point (PI) was 6.38.The protein contains ten transmembrane domains,and the secondary structure of ClHA2 was mainly consisted of α-helix. The results of quantitative real-time PCR showed that ClHA2 gene of three Chinese fir clones was expressed in roots ,stems and leaves under low-phosphorus stress,and its expression was inhibited to some extent.
|
|
Received: 03 April 2018
Published: 20 November 2018
|
|
|
|
|
|
|
