Abstract The COR413 (cold-regulated) gene, which encodes a plant-specific cold responsive protein, plays a critical role in cold acclimation. To determine the functional characterization of COR413 genes in response to cold stress, integrated grapevine (Vitis vinifera) genomic approaches of data mining and homologous cloning were used to obtain a total of 4 COR413 gene family members from cold-tolerant Vitis amurensis, designated as VaCOR413-PM2A、-PM1X1、-IM1 and -PM2B, respectively. Sequence analysis revealed that the 4 COR413 family members from V. amurensis showed significant nucleotide (97.2%~100%) and amino acid (99.5%~100%) similarity with those from V. vinifera, and the nucleotide differences were several single nucleotide substitutions. All COR413 proteins from V. amurensis contained the conserved domain WCOR413, several transmembrane motifs and Serine/Threonine/Tyrosine phosphorylation sties, where a predicted N-terminal signal peptide was only found in VaCOR413-PM1X1 and a glycosylphosphatidylinositol (GPI) anchor was only observed in VaCOR413-PM2B. The 4 members of COR413 from 2 grapevine species can be grouped into 2 distinct clusters, where COR413-PM2A, -PM1X1 and -PM2B were grouped together and -IM1 fall into another cluster. Moreover, tissue-specific expression profile of COR413 gene family members and their responses to cold stress were analyzed in both V. amurensis and V. vinifera cv. Cabernet Sauvignon. The results showed that VaCOR413-PM2A, -PM1X1, -IM1 and -PM2B displayed different tissue-specific features that were also not similar to those from V. vinifera. Compared to the corresponding gene expression profiles in V. vinifera, VaCOR413-PM2A, -PM1X1, -IM1 and -PM2B were detected in all tissues of V. amurensis with different expression intensities, among which VaCOR413-PM2A and -PM1X1 showed a relatively high expression in shoots, tendrils, flowers and young leaves, whereas no signals of VvCOR413-PM1X1 and -IM1 were detected in V. vinifera. Analysis of the gene expression profiles during the time-course of cold stress demonstrated the existence of 2 groups with different expression profiles in V. amurensis and 3 groups in V. vinifera. In one group of V. amurensis containing VaCOR413-PM2A, -PM2B and -PM1X1, a similar expression pattern was observed in with a decreased tendency following the time-course of cold stress, while VaCOR413-IM1 displayed a distinct expression profiles from those in group Ⅰ, and a high level of VaCOR413-IM1 expression was detected 24 h after cold stress with 9.57-fold than that of unstressed control. In V. vinifera, the expression profile of VvCOR413-PM2A was similar to VvCOR413-PM2B and their gene induction reached a maximum at 3 h in response to cold stress. VvCOR413-PM1X1 was rapid and transient in response to cold treatment, reached a maximum at 1 h, and then decreased. VvCOR413-IM1 displayed a high level of expression at 3 h of cold stress with 3.65-fold than that of control, quickly decreased at 6 h and maintained a relatively low level to the cold stress. Data from cold stress treatment suggested that VaCOR413-PM2A, -PM1X1 and -PM2B which were grouped together, displayed down-regulated expression in response to cold stress and the degree of expression level of these genes were generally lower than those from V. vinifera. Interestingly, VaCOR413-IM1 categorized into another group, showed a significant induction feature 24 h after cold stress. The results provide reference basis for exploring the possible function and mechanism of the members of the family in response to cold stress, which lays the theoretical for cold-(frozen-) tolerant molecular improvement in grapevine.
DING Xiao-Ling,ZHANG Ning-Bei,QIAO Shu-Zhen, et al. Cloning and Expression Analysis of COR413 Family Genes from Vitis amurensis in Response to Cold Stress[J]. , 2017, 25(3): 366-377.
[1]陈卫平, 尚红莺，刘效义，等.2007[J].宁夏贺兰山东麓酿酒葡萄冻害原因分析及防治措施北方园艺, 2007, 0(6):109-110 [2]邓江明，简令成.2001. 植物抗冻机理研究新进展: 抗冻基因表达及其功能[J]. 植物学通报 18(5): 521-530. (Deng J M, Jian L C, et al.2001.Advances of Studies on Plant Freezing:tolerance Mechanism:Freezing Tolerance Gene Expression and Its Function[J].Chinese Bulletin of Botany, 18(5): 521-530.)[J].植物学通报, 2001, 18(5):521-530 [3]贺善超, 罗国光.1999. 葡萄学[M].北京: 中国农业出版社.(He S L, Luo G G.1999.Enology[M].Beijing: China Agriculture Press.) [4]刘崇怀, 姜建福, 樊秀彩, et al.2014[J].中国野生葡萄资源在生产和育种中利用的概况植物遗传资源学报, 2014, 0(4):720-727 [5]Breton, G., J. Danyluk, et al. 2003. Expression profiling and bioinformatic analyses of a novel stress-regulated multispanning transmembrane protein family from cereals and Arabidopsis[J]. Plant physiology, 132(1): 64-74.[J].Plant physiology, 2003, 132(1):64-74 [6]Dombrowski, J.E. and R. C. Martin .2009. Evaluation of reference genes for quantitative RT-PCR in Lolium temulentum under abiotic stress[J]. Plant science, 176(3): 390-396.[J].Plant science, 2009, 176(3):390-396 [7]Frenette Charron, J.-B., G. Breton, et al. 2002. Molecular and structural analyses of a novel temperature stress‐induced lipocalin from wheat and Arabidopsis[J]. FEBS letters, 517(1-3): 129-132.[J].FEBS letters, 2002, 517(1-3):129-132 [8]Gordon-Kamm, W.and P. Steponkus .1984. The influence of cold acclimation on the behavior of the plasma membrane following osmotic contraction of isolated protoplasts[J].Protoplasma, 123(3): 161-173.[J].Protoplasma, 1984, 123(3):161-173 [9]Guo, X.-H., J. Jiang, et al. 2009. A ThCAP gene from Tamarix hispida confers cold tolerance in transgenic Populus (P. davidiana× P. bolleana)[J].Biotechnology letters, 31(7): 1079-1087.[J].Biotechnology letters, 2009, 31(7):1079-1087 [10]Hannah, M.A., D. Wiese, et al. 2006.Natural genetic variation of freezing tolerance in Arabidopsis[J].Plant physiology, 142(1): 98-112.[J].Plant physiology, 2006, 142(1):98-112 [11]Li, H., Y. Wang, et al. 2009. Identification of genes responsive to salt stress on Tamarix hispida roots[J].Gene, 433(1): 65-71.[J].Gene, 2009, 433(1):65-71 [12]Machuka, J., S. Bashiardes, et al. 1999.Sequence analysis of expressed sequence tags from an ABA-treated cDNA library identifies stress response genes in the moss Physcomitrella patens[J].Plant and Cell Physiology, 40(4): 378-387.[J].Plant and Cell Physiology, 1999, 40(4):378-387 [13]Mayer, B.F., M. A. Ali‐Benali, et al. 2015. Cold acclimation induces distinctive changes in the chromatin state and transcript levels of COR genes in Cannabis sativa varieties with contrasting cold acclimation capacities[J].Physiologia plantarum, 155(3): 281-295.[J].Physiologia plantarum, 2015, 155(3):281-295 [14]Steponkus, P.L. 1984. Role of the plasma membrane in freezing injury and cold acclimation[J]. Annual Review of Plant Physiology, 35(1): 543-584.[J].Annual Review of Plant Physiology, 1984, 35(1):543-584 [15]Tremblay, K., F. Ouellet, et al. 2005. Molecular characterization and origin of novel bipartite cold-regulated ice recrystallization inhibition proteins from cereals[J]. Plant and Cell Physiology, 46(6): 884-891.[J].Plant and Cell Physiology, 2005, 46(6):884-891 [16]Wang, J., K.-J. Zuo, et al. 2007. Isolation and bioinformatics analyses of a COR413-like gene from Gossypium barbadense[J].Acta Physiologiae Plantarum, 29(1): 1-9.[J].Acta Physiologiae Plantarum, 2007, 29(1):1-9 [17]Xu, W., R. Li, et al. 2014.Transcriptome profiling of Vitis amurensis, an extremely cold-tolerant Chinese wild Vitis species, reveals candidate genes and events that potentially connected to cold stress[J].Plant molecular biology, 86(4-5): 527-541.[J].Plant molecular biology, 2014, 86(4-5):527-541
