不同外源诱导子对三角褐指藻lcyb基因转录及其岩藻黄素含量的影响

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Abstract Lycopene beta-cyclase (LCYB), which catalyzes the formation of β-carotene and its oxides, is an enzyme act as branching point of the plant carotenoid synthesis pathway. This study investigated the effect of elicitors on the expression of lcyb gene and content of fucoidin in Phaeodactylum tricornutum. Firstly, the full-length cDNA of lcyb had been obtained from P. tricornutum, and bioinformatics analysis was carried out. Then, aethyl jasmonate (MeJA), aacetylsalicylic acid (ASA)，arachidonic acid (AA) and ammonium cerous sulfate (ACS) were used to induce the expression of Ptlcyb gene and the content of fucoxanthin in P. tricornutum. The results showed that the lcyb gene of Phaeodactylum tricornutum obtained by de novo sequencing was same as the sequence on NCBI(GenBank No. XM002176576). The full length of lcyb gene was comprised by 2 060 bp, contanting a 1 980 bp open reading fragment(ORF), which encoded a polypeptide of 659 amio acids. Sequence analysis showed that P. tricornutum LCYB had a dinucleotides-biding site at the N-terminus and a Predicted TM helix at the C-terminus. In addition, signal peptide and the presence of chloroplast peptide han been found, which further proved that LCYB was located in the thylakoid membrane. Phylogenetic tree demostrated that diatom such as P. tricornutum coexisted in an evolutionary branch, which was closely related. Upon the observation of Ptlcyb regulation expression, the expression level of Ptlcyb had significant rise when the concentration was 50 μmol/L MeJA, 10 mg/L ASA, 0.1 mg /L AA and 0. 4 mg/L ACS. At the same time, the content of fucoxanthin in P. tricornutum was significantly higher than that in the control group (P ＜0.01). The variation trend of fucoxanthin content and Ptlcyb expression were basically the same, which showed that Ptlcyb played an important role in the synthesis of fucoxanthin. This study provide theoretical guidance for the regulation of fucoxanthin synthesis and also provide a reference for further improving the content of fucoxanthin by means of metabolic engineering in P. tricornutum.

Key words： Phaeodactylum tricornutum Lycopene β-cyclase (LCYB) fucoxanthin Bioinformatics

Received: 24 March 2017 Published: 10 December 2017

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	YU Kai
	GONG Yi-Fu
	ZHU Shuai-Qi
	LIU Gao
	YU He-Yu

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YU Kai,GONG Yi-Fu,ZHU Shuai-Qi, et al. Effects of Different Exogenous Elicitors on lcyb Gene Transcription and Fucoxanthin Content in Phaeodactylum tricornutum[J]. , 2017, 25(12): 2009-2017.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2017/V25/I12/2009

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