三个绵羊品种BMPR-IB基因CDS区1113位点的多态性与产羔数的相关性分析

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Abstract The fecundity of sheep (Ovis aries) is controlled by the dominant gene, and the bonemorphogenetic protein type IB gene (BMPR-IB) has been identified as one of the dominant genes in multiparous ewes. To provide a theoretical basis for breeding for sheep fecundity and markers, the polymerase chain reaction single strand conformation polymorphism (PCR) was used to analysis the single nucleotide polymorphism (SNP) detection at 1113 loci in the bonemorphogenetic protein type IB (BMPR-IB) gene coding region of 3 breeds of sheep, including 188 Mongolian sheep, 112 Gansu Alpine Merino sheep and 48 Small Tailed Han sheep, then we also analyze the correlation among the polymorphism of 3 breeds of sheep BMPR-IB gene and litter size, in order to provide the material for studying the mechanism of prolificacy in sheep. The result showed that 3 different genotypes were found in Mongolian sheep and Gansu Alpine Merino sheep, including wild type called AA, homozygous mutant called BB and heterozygous mutant called AB, AB was the dominant genotype. 2 different genotypes were found in Small Tailed Han sheep, BB and AB, BB was the dominant genotype. With the analysis of polymorphism information content, it was shown that Small Tail Han sheep belonged to low polymorphism (PIC＜0.25), the other two breeds of sheep belonged to moderate polymorphism (0.25＜PIC＜0.5). There was a significant difference in the 1113 locus of BMPR-IB gene coding region among these 3 different breeds of sheep, this site may be related to the litter size of sheep so it can be used as a marker loci for reproductive traits of sheep and further theoretical basis for the production practice.

Key words： BMPR-IB gene Small Tail Han ewes Mongolia ewes Gansu alpine merino ewes Polymorphism

Received: 02 June 2017 Published: 10 December 2017

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	Articles by authors
	DU Li
	ZHANG Li-Beng
	ZHANG Xiao-Yan
	ZHU Shao-Hua
	MA Xiao-Meng

Cite this article:

DU Li,ZHANG Li-Beng,ZHANG Xiao-Yan, et al. The Correlation Analysis Between Polymorphism and Fecundity of 1113 Locus in BMPR-IB Gene CDS Area of Three Sheep (Ovis aries) Varieties[J]. , 2017, 25(12): 1989-1997.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2017/V25/I12/1989

[1] Galloway SM, McNatty KP, et al.Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovuLation rate and infertility in a dosage-sensitive manner[J]., 2000, 25(3):279-283 [2] Lan ZJ, Gu P, et al.GCNF-dependent repression of BMP-15 and GDF-9 mediates gamete regμLation of female fertility[J]., 2003, 22(16):4070-4081 [3] Fang X, Xu H, et al.Polymorphism in BMP4 gene and its association with growth traits in goats[J]., 2009, 36(6):1339-1344 [4] Monget P, Fabre S, et al.RegμLation of ovarian folliculogenesis by IGF and BMP system in domestic animals[J]., 2002, 23(1-2):139-154 [5] Murakami M, Matsuzaki F, et al.RegμLation of melanin synthesis by the TGF-beta family in B16 melanoma cells[J]., 2009, 36(6):1247-1250 [6]Shimasaki S, Moore R K, Erickson G F, et al.Ovarian bone morphogenetic proteins in female reproduction[J].International Congress, 2004, 1266(1266):241-247 [7] Yi SE, LaPolt PS, et al.The type I BMP receptor BmprIB is essential for female reproductive function[J]., 2001, 98(14):7994-7999 [8] MuLsant P, Lecerf F, et al.Mutation in bone morphogenetic protein receptor-IB is associated with increased ovuLation rate in Booroola Merino ewes[J]., 2001, 98(9):5104-5109[J]., 2001, 98(9):5104-5109 [9]Souza CJ, et al.The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1B (BMPR1B) gene[J].J Endocrinol, 2001, 169(2):R1-R1 [10]Wilson T, et al.Highly prolific Booroola sheep have a mutation in the intracellμLar kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granμLose cells[J].Biol Reprod, 2001, 64(4):1225-1225 [11]徐小波, 王公金, 窦德宇, 等.湖羊、小尾寒羊及其杂交后代-基因多态性[J].华北农学报, 2010, 25(3):52-54 [12]柳淑芳, 姜运良, 杜立新.和基因作为小尾寒羊多胎性能候选基因的研究[J].遗传学报, 2003, 30(8):755-760 [13]储明星, 桑林华, 王金玉, 等.小尾寒羊高繁殖力候选基因和的研究[J].遗传学报, 2005, 32(1):38-45 [14]王根林, 毛鑫智, , 等.分析发现我国湖羊和小尾寒羊存在多胎基因[J].南京农业大学学报, 2003, 26(1):104-106 [15] 王生宝, 张英杰, 刘月琴, 等.不同绵羊品种BMPR-IB基因多态性与产羔数的关系研究[J]., 2009, (12):8-10[J]., 2009, 0(12):8-10 [16] 闫亚东, 储明星, 等, .小尾寒羊和湖羊高繁殖力候选基因BMPR-IB的研究[J]., 2005, 13(1):66-71 [17]Asadpour R, Jafari-joozani R, et al.Detection of polymorphism in Booroola gene and its association with litter size in zel sheep breed in Iran[J].Journal of Animal Ecology, 2012, 45,(2):63-66 [18]柳淑芳, 闫艳春, 杜立新.基因及-基因的突变特性与生物学意义[J].遗传, 2003, 30(8):755-760 [19]Kimchi-Sarfaty C, Oh JM, Kim IW, et al.A “Silent” polymorphism in the MDR1 gene changes substrate specificity[J].Animal Science, 2007, 315(5811):525-528