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| 90 Days Feeding Test of SD Rats (Rattus norvegicus) by Transgenic Antitrypsin Gene Rice (Oryza sativa) |
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Abstract g authors, xhmei0791@hotmail.com
Abstract Antitrypsin is a major trypsin inhibitor in the human body. The lack of antitrypsin can lead to various diseases such as emphysema and respiratory distress syndrome, and the demand quantity for this kind of drugs is large, so the recombinant anti-trypsin has become a hot spot, the recombinant anti-trypsin has been expressed from prokaryotes such as Escherichia coli, eukaryotes such as yeast, plants such as rice(Oryza sativa) and animals such as goats(Capra hircus). But the safety of the recombinant antitrypsin should be concerned. To evaluate the safety of transgenic antitrypsin rice, the transgenic rice and its non-transgenic counterpart were fed to Sprague-Dawley(SD) rats (Rattus norvegicus) during 90 d. This study added the genetically modified (GM) rice (JY150248) and its non-transgenic control rice (JY150250) in a mass ratio of 17.5%, 35.0% and 70.0% respectively to the basal feed. The influence and safety of GM rice were evaluated by monitoring SD rats' nutrition, physiological status and biochemical indicators. During the 90 d feeding for SD rats, the body weight and food intake were monitored. At the end of the study, we surveyed the effects of physiological indexes, such as body weight, hematology and serum biochemistry indexes, and dissected the animals for pathological observation and calculated viscera coefficient. After 90 days, animals of each group were in good condition. There were several significant differences between GM and its corresponding non-GM group in indexes of the serum biochemistry, hematology and relative organ weight (P＜0.05), but these differences were not dose-relative or gender-relative. For which it meant no biological significance. The study does not find genetically modified rice has subchronic toxicity in experimental animals. The results also lay a scientific basis for the development of antitrypsin drugs using transgenic means.
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Received: 27 March 2017
Published: 01 November 2017
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SHEN Xue-Jing, ZHANG Er-Qin, XU Qian-Ru, LIU Lin-Ke, WAN Bo, LIU Yun-Chao, SUN Ya-Ning, ZHAO Dong, NIU Xiang-Xiang, DENG Rui-Guang, ZHANG Gai-Ping. Expression of Rabies virus G Protein in Rice (Oryza sativa) and Identification of Its Genetic Stability[J]. 农业生物技术学报, 2019, 27(2): 204-211. |
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