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| Analysis of MIKC-type MADS-box Gene Family in Wheat (Triticum aestivum) |
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2. National Center of Engineering and Technological Research for Wheat, Henan Agricultural University
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Abstract Homeotic gene family with MADS-box is widely involved in the growth and development of plant and plant morphology construction. MADS-box genes can be classified into types I and type Ⅱ, type Ⅱ genes are also named as MIKC type. In this study, the MIKC type genes, from wheat(Triticum aestivum), rice(Oryza sativa) and arabidopsis (Arabidopsis thaliana) from genome databases published in plant transcription factor family databases, were carried out with multialignment and phylogenetic analysis. Total 51 MIKC family genes were screened out from Chinese Spring genomic sequence data. These MIKC type genes were classified into agamous(AG), suppressor of overexpression of constans(SOC), agamous-like gene 6(AGL6), sepallata/agamous-like gene 2(SEP/AGL2), short vegetative phase(SVP), pistillata(PI), apetala3(AP3), gnetum gnemon mads13(GGM13), agamous-like gene 12(AGL12), agamous-like gene 17(AGL17) and squamosa/apetala 1(SQUA/AP1) subfamilies And the number of genes in these subfamilies were 8, 2, 3, 6, 2, 3, 4, 8, 5, 5 and 5 respectively. Moreover. total 105 MIKC type genes were identified from the wheat amino acid sequences in NCBI database. Among them, 12 genes belonged to classA (SQUA/AP1 subfamily), 10 genes belonged to classB (AP3, PI and GGM13 subfamily), 7 genes belonged to classC (AG subfamily), 7 genes belonged to classD (AG subfamily), 28 genes belonged to classE (SEP/AGL2subfamily) according to ABCDE homeotic gene model. The remaining 41 genes belong to SOC, AGL6, SVP, AGL12, AGL17, and OsMADS32 subfamilies, the number of genes in these subfamilies were 11, 5, 12, 2, 8, and 3 respectively. Local blast analysis was carried out using wheat MIKC type full length transcripts identified above, total 46 MIKC type unigenes were screened out from the transcriptome data of the dwarf, multi-pistil and male sterility (dms) mutant. Cluster, phylogenetic and expression pattern analysis were carried out for the MIKC type unigenes. Five genes in class A, TaAP1-1 (T1_60629), TaAP1-2 (T4_56463), TaAP1-3 (T1_42731), TaAP1-3 (T4_52821) and TaAP1-3 (T2_46748), expressed highly in wheat young spikes than that in the young stem tips. Compared with the normal plants, genes TaAP1-2 (T4_56463) and TaSEP-5A (T2_44069) were down-regulated, genes TaPI-1 (T4_15141), TaMADS82 (T3_871), TaAG-2B (T2_43881), TaSEP-2B (T4_38414), Tam7 (T2_37491), TaSEP-4 (T4_8204) and TaMADS12 (T4_56460) were up-regulated in dms mutant. These genes may be associated with abnormal differentiation of wheat flower organs. These results provide useful information for further studies on MIKC type genes in wheat development.
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Received: 18 April 2017
Published: 01 November 2017
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