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| Effect of SAHA on Development Potency of Oocytes and Embryos of Buffalo (Bubalus bubalus) |
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Abstract There is dramatic changing of histone modifications during in vitro maturating of oocytes, the changing of its expression pattern plays an important role on oocyte maturation and subsequently embryonic development. Suberoyl anilide hydroxamic acid (SAHA) acting as a anticancer drug is the potential inhibitor of histone deacetyltransferase, inhibiting the growth, differentiation and apoptosis of various types of tumor cell in vivo and vitro. To explore the effect of inhibitor of histone deacetyltransferase SAHA in vitro maturation and embryonic development potential of buffalo (Bubalus bubalus) oocytes, effects of maturing rate (first polar body discharge rate), cleavage rate of fertilized eggs, blastocyst rate and total cell number of blastocyst of buffalo oocytes were compared by different concentrations of (0, 2, 5, 10, 20 and 40 nmol/L) SAHA treatment. Results showed that maturing rate, cleavage rate, and blastocyst rate of group 10 nmol/LSAHA was significantly higher than control group ((77.07±1.79)% and (62.3±2.08)%, (86.81±1.93)% and (74.86±2.07)%, (27.56±2.86)% and (24.23±1.74)%, P＜0.05). As the concentration increasing, total cell number of blastocyst in each treatment group had a tendency to increase, but had no significantly difference with each other (141±10, 151±13, 165±17, 170±14, 147±20 and 185±22, P＞0.05). qRT-PCR tests revealed that the expression of (cAMP responsive element binding protein, CREB) binding protein gene (CBP), histone acetyltransferases 1(HAT1) gene and histonedeacetylase 1(HDAC1) gene in MⅡ stage oocytes of treatment groups were significantly reduced, though p300 was increased. In conclusion, histone deacetylase inhibitors SAHA was one of the most effective way to promote maturation of buffalo oocytes and embryonic development in vitro, this result will provid a theoretical basis for future study about gene regulatory mechanism of transgenic cloned embryonic-developmental.
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Received: 02 February 2016
Published: 22 July 2016
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| [1] MEMILI E, . and FIRST N L. Zygotic and embryonic gene expression in cow: A review of timing and mechanism of early gene expression as compared with other species[J]. Zygote, 2000. 8(1): 87-96.[2] 刘丽梅, 猪卵母细胞体外成熟及早期胚胎发育过程中组蛋白乙酰化修饰作用的研究[D], 2012, 吉林大学.[3] SENGUPTA N and SETO E. Regulation of histone deacetylase activities[J]. Journal Of Cellular Biochemistry, 2004. 93(1): 57–67.[4] CARROZZA M J, UTLEY R T, WORKMAN J L, et al. The diverse functions of histone acetyltransferase complexes[J]. Trends in Genetics, 2003. 19(19): 321-9.[5] YANG X J. The diverse superfamily of lysine acetyltransferases and their roles in leukemia and other diseases[J]. Nucleic Acids Research, 2004. 32(3): págs. 959-976.[6] TIM T and VOSS A K. The diverse biological roles of MYST histone acetyltransferase family proteins[J]. Cell Cycle, 2007. 6(6): 696-704.[7] ENDO T, KANO K and NAITO K. Nuclear Histone Deacetylases Are Not Required for Global Histone Deacetylation During Meiotic Maturation in Porcine Oocytes[J]. Biology of reproduction, 2008. 78(6): 1073-1080.[8] CHRIVIA J C, KWOK R P, LAMB N, et al. Phosphorylated CREB binds specifically to the nuclear protein CBP[J]. Nature, 1993. 365(6449): 855-859.[9] GIORDANO A and AVANTAGGIATI M L. p300 and CBP: partners for life and death[J]. Journal of cellular physiology, 1999. 181(2): 218-30.[10] GARCEA R L and ALBERTS B. Comparative studies of histone acetylation in nucleosomes, nuclei, and intact cells. Evidence for special factors which modify acetylase action[J]. Journal of Biological Chemistry, 1980. 255(23): 11454-11463.[11] SURES I and GALLWITZ D. Histone-specific acetyltransferases from calf thymus. Isolation, properties, and substrate specificity of three different enzymes[J]. Biochemistry, 1980. 19(5): 943-951.[12] PARK I-K, HE Y, LIN F, et al. Differential gene expression profiling of adult murine hematopoietic stem cells[J]. Blood, 2002. 99(2): 488-498.[13] 庄涵虚, 马旭东, 赖亚栋等. RNA 干扰沉默 HDAC1 基因对胃癌细胞增殖, 凋亡, 组蛋白乙酰化和甲基化的影响[J]. 南方医科大学学报, 2014. 34(2): 246-250.[14] WANG Q, YIN S, AI J-S, et al. Histone deacetylation is required for orderly meiosis[J]. Cell Cycle, 2006. 5(7): 766-774.[15] LI Y, BUTENKO Y and GRAFI G. Histone deacetylation is required for progression through mitosis in tobacco cells[J]. The Plant Journal, 2005. 41(3): 346-352.[16] 刘峰, HDACl 基因, PITl 基因与猪部分经济性状相关性研究 [D] 2005, 湖南农业大学.[17] 柳小春, 肖调义, 何伟光等. 猪生长及胴体性状 3 个相关基因座遗传效应[J]. 中国农业科学, 2009. 42(2): 742-747.[18] 杨颖, HDAC1 基因多态位点与肉牛部分经济性状的相关性分析[D], 2012, 广西大学. |
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