大口黑鲈PACAP基因SNPs的筛选及其与生长性状的关联分析

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Abstract Pituitary adenylate cyclase-activating polypeptide (PACAP), a member of glucagon/secretes peptide family, is a pleiotropic hormone that enhances and stimulates the secretion of growth hormone, gonadotropin, prolactin and somatolactin. The single nucleotide polymorphism (SNP) in PACAP gene can affect its function or translation and transcription. Studying the association between the SNP and growth traits can provide candidate markers for marker-assisted selection. We detected the polymorphism of PACAP gene by DNA sequencing to explore the association between the polymorphism of PACAP and growth traits of largemouth bass (Micropterus salmoides). Five pairs of primers were designed to amplify the PACAP gene sequence, and a SNP A-2282C site was found in the 4th intron, and had 3 genotypes named as AA, AC and CC, respectively. The genotype and gene frequency of this SNP in 327 largemouth bass were further assayed with the method of Snapshot, the result showed that gene frequency of genotypes AA, AC and CC were 0.034, 0.645 and 0.321, respectively, and the frequency of A allele and C allele were 0.355 8 and 0.644 2. Chi-square test showed that the locus was in Hardy-Weinberg equilibrium in the largemouth bass population，the effective number(Ne) of the locus was 1.846 5, expected heterozygosity (Ho) and observed heterozygosity (He) were 0.540 9 and 0.644 2. The least squire method (LSM) was used to analyze the association of genotype with growth traits, and variance analysis indicated the mean values of AC genotype group was higher than that of AA and CC groups in body weight, body length, head length, total length, body depth and caudal peduncle length. And the body width and caudal peduncle length with AC genotype were significantly higher than that of AA (P＜0.05). The depth with AC genotype was extremely significantly higher than group with CC (P＜0.01), and those with AC genotype were significantly higher than those with AA genotype (P＜0.05). The locus can be a candidate molecular marker for largemouth bass breeding.

Key words： Largemouth bass Pituitary adenylate cyclase-activating polypeptide (PACAP) SNP Growth

Received: 10 September 2015 Published: 05 February 2016

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	Articles by authors
	LIU Gao
	BO Jun-Jie
	LI Qing-Jie
	MA Dong-Mei
	QUAN Ying-Chun

Cite this article:

LIU Gao,BO Jun-Jie,LI Qing-Jie, et al. SNPs Detection of PACAP Gene and Its Association with Growth Traits in Largemouth Bass (Micropterus salmoides)[J]. , 2016, 24(3): 390-396.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2016/V24/I3/390

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