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Expression and Cellular Localization of CDK1 and CyclinB1 in the Sheep(Ovis aires)Testes |
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Abstract Abstract The meiotic maturation of spermatocyte in mammal is controlled by the maturation promotion factor (maturation promoting factor, MPF), a complex of CDK1(cyclin-dependent kinase, CDK1) and CyclinB1 proteins. In the present study, we selected 0,2,6,12,24-month-old sheep testis to investigate the expression and cellular localization of CDK1 and CyclinB1 in the sheep testes on different months . CDK1 and CyclinB1 genes expression levels in different months testis were analyzed by qRT-PCR(fluorescence quantitative Realtime PCR,qRT-PCR) and CDK1 and CyclinB1 localization in testis were detected by immunohistochemistry. The results showed that with month increasing, the expression of CDK1 and CyclinB1 mRNA were firstly decreased and then increased. CDK1 and CyclinB1 mainly localized in the spermatogonia, primary spermatocytes and secondary spermatocytes. CDK1 protein always expressed at an extremely low level throughout all stage of development. However, its expression quantity was relatively stable. On the other side, the monthly expression difference of CyclinB1 protein in sheep testis of were remarkable, the expression level was always higer than that of CDK1 protein. These results indicated that the expression of CDK1 and CyclinB1 mRNA and protein in sheep testes at different months were significant. And it may play an important role in sheep testis development.
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Received: 24 July 2015
Published: 05 February 2016
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[1]范丽.2010. 猪CyclinA基因的克隆及其功能的初步研究[D]. 硕士学位论文,西北农林科技大学, 导师:张彦明.pp.14-44.(Fan l.2010. Cloning and Preliminary Functional Research of CyclinA Gene in Pig[D]. Thesis for M.S., North West Agriculture and Forestry University, Suppervisor:Zhang Y M, pp.14-44.)[2]梁卫红. 2012. 细胞生物学[M]. 科学出版社, 北京. pp.235-270.(Liang W H.2012. Cell Biology[M]. Science Press, Beijing, China. pp. 235-270.)[3]姚晓磊, 宋瑞高, 吕丽华, 等.2015. 不同日龄犊牛睾丸中细胞周期调控基因 CyclinB1 和p34 cdc2 表达的差异[J]. 畜牧兽医学报, 46(2): 245-250.(Yao X L, Song R G, Lv L H, et al. 2015. Expressions of Cell Cycle Regulation Genes CyclinB1 and p34cdc2 in the Testes of Calves Aged on Different Days[J]. Acta Veterinaria et Zootechnica Sinica, 46(2): 245-250.)[4]曾琛.2006. 不同倍性鱼cdc2基因cDNA部分序列的克隆及其在卵巢中的表达分析[D]. 硕士学位论文, 湖南师范大学, 导师:刘少军.pp.16-36.(Zen C.2006. Partial cDNA Cloning, Comparative Analysis of Transcriptional Level of cdc2 Gene in Ovaries with Different Ploidy Level Cyprinid Fish[D]. Thesis for M.S., (Hunan Normal University, Suppervisor:Liu S J, pp.16-36.)[5]翟中和, 王喜忠, 丁孝明. 2007. 细胞生物学[M].高等教育出版社, 北京. pp.385-437.(Zhai Z H, Wang X Z, Ding X M. 2007. Cell Biology[M]. Higher Education Press, Beijing, China. pp.385-437.)[6]Ambros V.2009. pRB/CKI pathways at the interface of cell cycle and development.[J]. Cell Cycle, 8(21):3433-3434.[7]Bloom J, Cross F R. 2007. Multiple levels of cyclin specificity in cell-cycle control[J]. Nature Reviews Molecular Cell Biology, 8(2): 149-160.[8]Chiu A. 2010. Review of chromium (VI) apoptosis, cell-cycle-arrest, and carcinogenesis.[J]. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev, 28(3):188-230.[9]Han K, Dai Y, Zou Z, et al.2012. Molecular characterization and expression profiles of cdc2 and cyclin B during oogenesis and spermatogenesis in green mud crab (Scylla paramamosain).[J]. Comp Biochem Physiol B Biochem Mol Biol, 163:292–302.[10]Johnson R T, Rao P N, Hughes H D, et al. 1970. Mammalian cell fusion III. A HeLa cell inducer of premature chromosome condensation active in cells from a variety of animal species[J]. Journal of cellular physiology, 76(2): 151-157.[11]Kim W Y, Sharpless N E.2006. The regulation of INK4/ARF in cancer and aging.[J]. Cell, 127(2):265-275.[12]Masui Y, Markert C L. 1971. Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes[J]. Journal of Experimental Zoology, 177(2): 129-145.[13]Qiu G F, Ramachandra R K, Yao J, et al. 2008. Molecular characterization and expression profiles of cyclin B1, B2 and Cdc2 kinase during oogenesis and spermatogenesis in rainbow trout (Oncorhynchus mykiss).[J]. Animal Reproduction Science, 105(3-4):209-225.[14]Ren Y, Wang Q, Shi L, et al.2011. Effects of maternal and dietary selenium (Se-enriched yeast) on the expression of p34(cdc2) and CyclinB1 of germ cells of their offspring in goats.[J]. Animal Reproduction Science, 123:187-191.[15]Ubersax, Woodbury, Quang, et al. 2003. Targets of the cyclin-dependent kinase Cdk1.[J]. Nature, 425.[16]Yang J, Kornbluth S. 1999. All aboard the cyclin train: subcellular trafficking of cyclins and their CDK partners[J]. Trends in cell biology, 9(6): 207-210.[17]Yu B, Song Y, Yu D, et al.2006. Expression and immunohistochemical localization of Cdc2 and P70S6K in different stages of mouse germ cells.[J]. Cell Biochemistry & Function, 24(2):113–117.[18]Zeng Huawei.2002. Selenite and selenomethionine promote HL-60 cell cycle progression.[J]. Journal of Nutrition, 132(4):674-679. |
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