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| Effect of Soybean (Glycine max) GmGBP1 Gene on Transgenic Tobacco (Nicotiana tabacum) Seedlings Resistance to Heat Stress |
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Abstract Soybean (Glycine max) gibberellin-regulated MYB-related transcription factor binding protein 1 (GmGBP1) gene is a homologous gene of Sloan-Kettering retrovirus interacting protein (SKIP) gene . Proteins encoded by GmGBP1 not only participate in the regulation of a variety of abiotic stress signal transduction pathways in plants, but also play a role in the signal transduction of plant hormones, such as abscisic acid and gibberellin. In this study, soybean GmGBP1 gene was transformed into transgenic tobacco (Nicotiana tabacum) and overexpressed. After being treated at a high temperature of 48℃ for 48 h, leaf wilting and necrosis, leaf chlorosis and leaf etiolation were detected in the wild-type (WT) tobacco seedlings; while no obvious yellowing was found in the leaves of transgenic tobacco of overexpression GmGBP1 gene (GBP1ox) seedlings at a high temperature. After being treated at a high temperature of 55 ℃ for 12 h, tobacco seedlings were placed at room temperature for recovery growth for 15 d, and the survival rate of over-expression of GmGBP1 gene in tobacco seedlings was 24.14%. The survival rate of GBP1ox tobacco seedling was higher than the WT tobacco seedlings. The malondialdehyde (MDA) content of WT leaf tobacco seedlings was 2.07-fold more than that in GBP1ox after 48 ℃ treatment for 48 h. The leaves of tobacco seedlings were stained by using diaminobenzidine (DAB) after being treated at a high temperature, and the staining results demonstrated that the ratio of stained area of WT tobacco seedling leaves to total leaf area was 43.66%. The percentage of the total leaf area of DAB staining in WT seedling tobacco was significantly greater than GBP1ox tobacco seedlings (P＜0.05) as a result. High temperature treatment was performed in tobacco seedlings to analyze the changes in the relative expression levels of heat-resistance related genes in tobaccos. Zinc finger protein of Arabidopsis thaliana 12 (ZAT12) gene expression was significantly higher in GBP1ox leaf tobacco seedlings than that in WT (P＜0.05) for 3, 6, 7 and 8 h treatment at 55 ℃ high temperature. On the other hand, there was no significant difference in the relative expression levels of heat shock protein 40 (HSP40) gene in WT and GBP1ox the leaves of tobacco seedlings after being treated at a high temperature. The results showed that overexpression of GmGBP1 gene could improve the heat resistance of transgenic tobacco seedlings to some extent. The present study provides references for the cultivation of new heat-resistant transgenic crops, and lays a solid foundation on further investigations about the functions of soybean GmGBP1 gene.
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Received: 08 October 2015
Published: 05 February 2016
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