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| Isolation and Function Analysis of Heat Stress Related Transcription Factor Gene TabZIP28 in Wheat (Triticum aestivum) |
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Abstract Basic leucine zipper (bZIP) transcription factor family widely distributes in plant and involves in multiple stress response and developmental stages. In this study, we cloned a heat induced transcription factor gene TabZIP28 (GenBank accession No. KT753298.1) from wheat (Triticum aestivum), the length of ORF was 1 713 bp, encoding a protein of 570 amino acid residues. Sequence analysis indicated that TabZIP28 possessed a conserved bZIP domain, a transmembrane domain (TMD) and a canonical site 1 protease (S1P) cleavage site. The promoter region of TabZIP28 contained several stress related cis-element. The expression patterns of TabZIP28 were analyzed by qRT-PCR under stress conditions. The results showed that TabZIP28 was induced by heat and peaked at 1 h; the transcript level of TabZIP28 was increased to the highest level by 20% PEG treatment for 6 h and reduced rapidly after 12 h; TabZIP28 was found to be slightly up-regulated by treatment of 5 mmol/L H2O2 for 12 h and TabZIP28 could not be induced by DTT treatment. To investigate the function of TabZIP28, we overexpressed TabZIP28 in Arabidopsis thaliana and assessed the thermotolerance of the transgenic lines. The results showed that the survival rate and germination rate of transgenic lines after heat stress were statistically higher than the wild type. This indicated that TabZIP28 could contribute to heat stress tolerance and could be used as a candidate gene for breeding of thermotolerant cultivars.
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Received: 14 July 2015
Published: 29 December 2015
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