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| The Genetic Difference Between Hubei Black Boned-goat (Capra hircus) and the Other Three Neighboring Goat Breeds |
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1. Huazhong Agricultural University
2.
3. Huazhong Agricultural University
4. Southwest University
5. Chongqing Academy of animal sciences
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Abstract In order to analyse the situation of gene flow(Nm) and the degree of genetic differentiation, Hubei black-boned goat(Capra hircus) and other 3 geographical closely breeds of Youzhou black goat, Macheng black goat and Nanjiang yellow goat, as well as Boer goat as out group breed, were chosen in the preset research. Allele heterozygosity(h), Shannon-Weiner index(S) and the efficient allelic number(Ne) which can reflect population genetic diversity were calculated using 14 single nucleotide polymorphism (SNP) markers of the 5 goat breeds. Meanwhile, the situation of gene flow and genetic differentiation between Hubei black-boned goat and the other 3 local goat breeds were also analyzed, with the linear model relationship establishment between geographical distance and genetic differentiation. Moreover, the clustering figure of the 5 populations was built using the genetic distance (Ds) data. The results showed that Hubei black-boned goat had the highest value of both allele heterozygosity (h), Shannon-Weiner index (S) and the efficient allelic number (Ne) which suggested Hubei black-boned goat had the most abundant genetic variation. The also finding was that the genetic differentiation(Fst) was medium which ranged from 0.072 9 to 0.137 2 between Hubei black-boned goat and other 4 goat populations, but the gene flow(Nm) was large of which ranged from 1.571 6 to 3.178 7. Unweighted pair-group method with arithmetic means(UPGMA) based clustering figure showed that Hubei black-boned goat was put the outermost of the graph for large genetic differentiation. Otherwise, there was positive linear relationship between genetic differentiation and geographical distance, genetic distance(Ds) and geographical distance(D) also had correlation relationship (r=0.934). There were significant genetic differentiation between Hubei black-boned goat and the other 3 goat populations and Hubei black-boned goat had the most abundant genetic variation, but the large gene flow was adverse for this germplasm resources. So it is necessary to take some measures to prevent germplasm genetic resources from loss by hybridization and dilution. The result provides some clues for conservation and breeding of Hubei black-boned goat.
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Received: 27 October 2014
Published: 01 March 2015
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