苏云金芽胞杆菌FH21生物学特性及其cry1类基因的鉴定

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Abstract This study was based on Bacillus thuringiensis(Bt) strain FH21 with high toxic of Plutella xylostella larvae. Using an improved polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method, cry1Bb3, cry1Da4, cry1Ja3 and cry1Id3 genes were cloned from strain FH21 with the GenBank No. KJ619659, KJ619660, JQ228425 and KJ619661, respectively. The 4 genes were inserted into expression vector pEB, and transformed into Escherichia coli strain Rosetta (DE3), which were induced by isopropyl β-D-1-thiogalactopyranoside (IPTG) at low temperature. The total protein of FH21 and the 4 proteins were used for bioassay on P. xylostella, Helicoverpa armigera and Ostrinia furnacalis. The total protein of FH21 had high insecticidal activity against 3 insects with lethal concentration 50 (LC50) of 0.801, 0.502 and 2.858 mg/L, respectively. Cry1Bb3, Cry1Ja3 and Cry1Id3 had high insecticidal activity against P. xylostella and O. furnacalis with LC50 of 1.860, 1.697, 1.538, 1.800, 2.270 and 0.840 mg/L, respectively. This study showed that Bt FH21strain against agricultural pests with a high insecticidal activity, insecticidal genes were assayed and insecticidal activity were determined simultaneously. The cry1 genes provide more choices for important agricultural pest.

Key words： Bacillus thuringiensis cry1 genes Cloning Expression Insecticidal activity identification

Received: 29 May 2015 Published: 05 October 2015

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	Articles by authors
	LI Hai-Shou
	HE Bao-Nan
	DIAO Shi-Yuan
	LIU Rong-Mei
	ZHANG Jie
	GAO Ji-Guo

Cite this article:

LI Hai-Shou,HE Bao-Nan,DIAO Shi-Yuan, et al. Biological Characteristics of Bacillus thuringiensis FH21 Strain and Identification of Its cry1 Genes[J]. , 2015, 23(11): 1445-1451.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2015/V23/I11/1445

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