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| Establishment, Characterization of a New Cell Line From Heart of Kelp Bass (Epinephelus moara) |
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Abstract Cell lines provide an important in vitro system for investigations in virology and functional genomics in fish. A new heart cell line, epinephelus moara heart cell line (EMH), was established from Kelp Bass (Epinephelus moara). The cell line was maintained in Leibovitz's L15 medium supplemented with HEPES, antibiotics, 2-Mercaptoethanol (2-ME), fetal bovine serum (FBS) and basic fibroblast growth factor (bFGF). The cultured EMH cells, fibroblastic and epithelial in morphology, could be subcultured once every 4 or 5 days and had been subcultured for more than 20 passages. The suitable temperature for the cell growth was 18~30 ℃ with the optimum growth at 30 ℃ and an unfavorable growth at 36 ℃. The suitable FBS concentration for the cell growth was 10%~20% with the optimum concentration at 15%. Cytogenetic analysis revealed that 62% of the cells maintained a normal diploid chromosome number (2n=48) in the EMH cells at Passage 20. The fluorescent signals were observed in EMH cells after the cells were transfected with enhanced green fluorescent protein (EGFP) reporter plasmid. The EMH established here can be used as a useful tool for transgenic and genetic manipulation studies and may be potentially used for fish virus isolation, propagation and vaccine development.
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Received: 20 March 2015
Published: 24 July 2015
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