Identification of FLOWERING LOCUS C(FLC) Gene in 2# Chinese Cabbage(Brassica rapa ssp. pekinensis)-cabbage(B. oleracea var. capitata) Monosomic Alien Addition Line
Abstract Flower time is important trait in Brassica, FLCs are key genes to flowering. Forward primers were designed on polymorphic region of exon 4, reverse primers were designed on conserved region of exon 7. Four Specific primers were developed for Genotyping of BrFLC1, BrFLC2, BrFLC3, BrFLC5, BoFLC1, BoFLC2, BoFLC3 and BoFLC5. FLCs were identified by above mentioned specific primers in the Chinese cabbage monosomic alien addition line which introduced chromosome 2 from cabbage, the results showed that besides the 4 BrFLCs, BoFLC3 was existing in the monosomic alien addition line. Which provide new way to identification of BoFLCs in the background of Chinese cabbage, and supply generation of Chinese cabbage translocation lines added BoFLC3 with special materials.
